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Article

Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

1
Department of Biomedical and Clinical Sciences “L. Sacco”, University of Milan, Via Grassi 74, 20157 Milan, Italy
2
Pediatric Research Center “Romeo ed Enrica Invernizzi”, University of Milano, Via G.B. Grassi 74, 20157 Milano, Italy
3
Genomic and Post-Genomic Center, IRCCS Mondino Foundation, 27100 Pavia, Italy
4
Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, Italy
5
Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, 20133 Milano, Italy
6
Department of Biosciences, University of Milan, Via Celoria 26, 20133 Milano, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
These authors contributed equally to the work.
Int. J. Mol. Sci. 2020, 21(18), 6775; https://doi.org/10.3390/ijms21186775
Received: 30 July 2020 / Revised: 5 September 2020 / Accepted: 14 September 2020 / Published: 15 September 2020
(This article belongs to the Special Issue Mechanobiology in Cells and Tissues)
3D cell cultures are becoming more and more important in the field of regenerative medicine due to their ability to mimic the cellular physiological microenvironment. Among the different types of 3D scaffolds, we focus on the Nichoid, a miniaturized scaffold with a structure inspired by the natural staminal niche. The Nichoid can activate cellular responses simply by subjecting the cells to mechanical stimuli. This kind of influence results in different cellular morphology and organization, but the molecular bases of these changes remain largely unknown. Through RNA-Seq approach on murine neural precursors stem cells expanded inside the Nichoid, we investigated the deregulated genes and pathways showing that the Nichoid causes alteration in genes strongly connected to mechanobiological functions. Moreover, we fully dissected this mechanism highlighting how the changes start at a membrane level, with subsequent alterations in the cytoskeleton, signaling pathways, and metabolism, all leading to a final alteration in gene expression. The results shown here demonstrate that the Nichoid influences the biological and genetic response of stem cells thorough specific alterations of cellular signaling. The characterization of these pathways elucidates the role of mechanical manipulation on stem cells, with possible implications in regenerative medicine applications. View Full-Text
Keywords: mechanotransduction; scaffolds; neural stem cells; RNA-Seq; computational genomics; signal transduction; niche; mechanobiology; regenerative medicine mechanotransduction; scaffolds; neural stem cells; RNA-Seq; computational genomics; signal transduction; niche; mechanobiology; regenerative medicine
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MDPI and ACS Style

Rey, F.; Pandini, C.; Barzaghini, B.; Messa, L.; Giallongo, T.; Pansarasa, O.; Gagliardi, S.; Brilli, M.; Zuccotti, G.V.; Cereda, C.; Raimondi, M.T.; Carelli, S. Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction. Int. J. Mol. Sci. 2020, 21, 6775. https://doi.org/10.3390/ijms21186775

AMA Style

Rey F, Pandini C, Barzaghini B, Messa L, Giallongo T, Pansarasa O, Gagliardi S, Brilli M, Zuccotti GV, Cereda C, Raimondi MT, Carelli S. Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction. International Journal of Molecular Sciences. 2020; 21(18):6775. https://doi.org/10.3390/ijms21186775

Chicago/Turabian Style

Rey, Federica, Cecilia Pandini, Bianca Barzaghini, Letizia Messa, Toniella Giallongo, Orietta Pansarasa, Stella Gagliardi, Matteo Brilli, Gian V. Zuccotti, Cristina Cereda, Manuela T. Raimondi, and Stephana Carelli. 2020. "Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction" International Journal of Molecular Sciences 21, no. 18: 6775. https://doi.org/10.3390/ijms21186775

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