Next Article in Journal
Non-Coding RNAs as Cancer Hallmarks in Chronic Lymphocytic Leukemia
Previous Article in Journal
Recent Advances in Cell-Based Therapies for Ischemic Stroke
Article

Impact of KLF4 on Cell Proliferation and Epithelial Differentiation in the Context of Cystic Fibrosis

1
BioISI—Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, 1749-016 Lisbon, Portugal
2
Departments of Pediatrics, Gynecology & Obstetrics and of Cell Physiology & Metabolism, Geneva University Hospitals and Medical School of the University of Geneva, 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(18), 6717; https://doi.org/10.3390/ijms21186717
Received: 28 August 2020 / Revised: 10 September 2020 / Accepted: 11 September 2020 / Published: 14 September 2020
(This article belongs to the Section Molecular Biology)
Cystic fibrosis (CF) cells display a more cancer-like phenotype vs. non-CF cells. KLF4 overexpression has been described in CF and this transcriptional factor acts as a negative regulator of wt-CFTR. KLF4 is described as exerting its effects in a cell-context-dependent fashion, but it is generally considered a major regulator of proliferation, differentiation, and wound healing, all the processes that are also altered in CF. Therefore, it is relevant to characterize the differential role of KLF4 in these processes in CF vs. non-CF cells. To this end, we used wt- and F508del-CFTR CFBE cells and their respective KLF4 knockout (KO) counterparts to evaluate processes like cell proliferation, polarization, and wound healing, as well as to compare the expression of several epithelial differentiation markers. Our data indicate no major impact of KLF4 KO in proliferation and a differential impact of KLF4 KO in transepithelial electrical resistance (TEER) acquisition and wound healing in wt- vs. F508del-CFTR cells. In parallel, we also observed a differential impact on the levels of some differentiation markers and epithelial-mesencymal transition (EMT)-associated transcription factors. In conclusion, KLF4 impacts TEER acquisition, wound healing, and the expression of differentiation markers in a way that is partially dependent on the CFTR-status of the cell. View Full-Text
Keywords: KLF4; wound healing; epithelial differentiation; proliferation; cystic fibrosis KLF4; wound healing; epithelial differentiation; proliferation; cystic fibrosis
Show Figures

Figure 1

MDPI and ACS Style

Sousa, L.; Pankonien, I.; Simões, F.B.; Chanson, M.; Amaral, M.D. Impact of KLF4 on Cell Proliferation and Epithelial Differentiation in the Context of Cystic Fibrosis. Int. J. Mol. Sci. 2020, 21, 6717. https://doi.org/10.3390/ijms21186717

AMA Style

Sousa L, Pankonien I, Simões FB, Chanson M, Amaral MD. Impact of KLF4 on Cell Proliferation and Epithelial Differentiation in the Context of Cystic Fibrosis. International Journal of Molecular Sciences. 2020; 21(18):6717. https://doi.org/10.3390/ijms21186717

Chicago/Turabian Style

Sousa, Luís; Pankonien, Ines; Simões, Filipa B.; Chanson, Marc; Amaral, Margarida D. 2020. "Impact of KLF4 on Cell Proliferation and Epithelial Differentiation in the Context of Cystic Fibrosis" Int. J. Mol. Sci. 21, no. 18: 6717. https://doi.org/10.3390/ijms21186717

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop