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Open AccessArticle

Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces

1
Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS, 75005 Paris, France
2
Physico-chimie des Électrolytes et Nanosystèmes Interfaciaux, PHENIX, Sorbonne Université, CNRS, F-75005 Paris, France
3
Laboratoire Physico Chimie Curie, Institut Pierre Gilles de Gène, Institut Curie, PSL Research University, Sorbonne Université, CNRS, 75005 Paris, France
4
Guy Hilton Research Centre, School of Pharmacy and Bioengineering, Keele University, Stoke-on-Trent, Staffordshire ST4 7QB, UK
5
Department of Biochemistry II – Molecular Neurobiochemistry, Faculty of Chemistry and Biochemistry, Ruhr-Universität Bochum, 44801 Bochum, Germany
6
Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan
7
Healthcare Technology Institute, Institute of Translational Medicine, University of Birmingham, Birmingham B15 2TT, UK
8
Department of Biology/Chemistry, University of Osnabrück, Barbarastr. 11, 49076 Osnabrück, Germany
9
Institute of Pharmacy and Bioengineering, School of Medicine, Keele University, Keele ST5 5BG, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(18), 6560; https://doi.org/10.3390/ijms21186560
Received: 20 July 2020 / Revised: 1 September 2020 / Accepted: 4 September 2020 / Published: 8 September 2020
The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can act at a distance without interactions with the surrounding biological system. To control biological processes at a subcellular spatial resolution, magnetic nanoparticles can be used either to induce biochemical reactions locally or to apply forces on different elements of the cell. Here, we show that cell migration and neurite outgrowth can be directed by the forces produced by a switchable parallelized array of micro-magnetic pillars, following the passive uptake of nanoparticles. Using live cell imaging, we first demonstrate that adherent cell migration can be biased toward magnetic pillars and that cells can be reversibly trapped onto these pillars. Second, using differentiated neuronal cells we were able to induce events of neurite outgrowth in the direction of the pillars without impending cell viability. Our results show that the range of forces applied needs to be adapted precisely to the cellular process under consideration. We propose that cellular actuation is the result of the force on the plasma membrane caused by magnetically filled endo-compartments, which exert a pulling force on the cell periphery. View Full-Text
Keywords: nanoparticles; magnetic; cell migration; axonal outgrowth; endosomes; cell polarity; live cell imaging nanoparticles; magnetic; cell migration; axonal outgrowth; endosomes; cell polarity; live cell imaging
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    Doi: 10.5281/zenodo.3952663
    Link: https://zenodo.org/record/3952663#.XxW8cSgzZPY
    Description: Video S6: Parallelized magnetic control of Hela cells Video S7: Parallelized magnetic control of SHSY-5Y cells Video S8: Parallelized magnetic control of cortical neurons Video S9: Parallelized magnetic control of cortical neurons Video S10&S11: Parallelized magnetic control of PC12 cells Video S12: Lysosome labeling of Hela cells Video S13: Late endosome labeling of Hela cells
MDPI and ACS Style

Bongaerts, M.; Aizel, K.; Secret, E.; Jan, A.; Nahar, T.; Raudzus, F.; Neumann, S.; Telling, N.; Heumann, R.; Siaugue, J.-M.; Ménager, C.; Fresnais, J.; Villard, C.; El Haj, A.; Piehler, J.; Gates, M.A.; Coppey, M. Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces. Int. J. Mol. Sci. 2020, 21, 6560.

AMA Style

Bongaerts M, Aizel K, Secret E, Jan A, Nahar T, Raudzus F, Neumann S, Telling N, Heumann R, Siaugue J-M, Ménager C, Fresnais J, Villard C, El Haj A, Piehler J, Gates MA, Coppey M. Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces. International Journal of Molecular Sciences. 2020; 21(18):6560.

Chicago/Turabian Style

Bongaerts, Maud; Aizel, Koceila; Secret, Emilie; Jan, Audric; Nahar, Tasmin; Raudzus, Fabian; Neumann, Sebastian; Telling, Neil; Heumann, Rolf; Siaugue, Jean-Michel; Ménager, Christine; Fresnais, Jérôme; Villard, Catherine; El Haj, Alicia; Piehler, Jacob; Gates, Monte A.; Coppey, Mathieu. 2020. "Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces" Int. J. Mol. Sci. 21, no. 18: 6560.

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