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Interplay between Carbonic Anhydrases and Metallothioneins: Structural Control of Metalation

Department of Chemistry, The University of Western Ontario, 1151 Richmond St., London, ON N6A5B7, Canada
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(16), 5697; https://doi.org/10.3390/ijms21165697
Received: 3 July 2020 / Revised: 31 July 2020 / Accepted: 5 August 2020 / Published: 9 August 2020
(This article belongs to the Special Issue Carbonic Anhydrase and Biomarker Research 2020)
Carbonic anhydrases (CAs) and metallothioneins (MTs) are both families of zinc metalloproteins central to life, however, they coordinate and interact with their Zn2+ ion cofactors in completely different ways. CAs and MTs are highly sensitive to the cellular environment and play key roles in maintaining cellular homeostasis. In addition, CAs and MTs have multiple isoforms with differentiated regulation. This review discusses current literature regarding these two families of metalloproteins in carcinogenesis, with a dialogue on the association of these two ubiquitous proteins in vitro in the context of metalation. Metalation of CA by Zn-MT and Cd-MT is described. Evidence for protein–protein interactions is introduced from changes in metalation profiles of MT from electrospray ionization mass spectrometry and the metalation rate from stopped-flow kinetics. The implications on cellular control of pH and metal donation is also discussed in the context of diseased states. View Full-Text
Keywords: metallothioneins; carbonic anhydrase; protein–protein interactions; electrospray ionization mass spectrometry; metalation kinetics; metal donation; pH dependence metallothioneins; carbonic anhydrase; protein–protein interactions; electrospray ionization mass spectrometry; metalation kinetics; metal donation; pH dependence
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MDPI and ACS Style

Wong, D.L.; Yuan, A.T.; Korkola, N.C.; Stillman, M.J. Interplay between Carbonic Anhydrases and Metallothioneins: Structural Control of Metalation. Int. J. Mol. Sci. 2020, 21, 5697. https://doi.org/10.3390/ijms21165697

AMA Style

Wong DL, Yuan AT, Korkola NC, Stillman MJ. Interplay between Carbonic Anhydrases and Metallothioneins: Structural Control of Metalation. International Journal of Molecular Sciences. 2020; 21(16):5697. https://doi.org/10.3390/ijms21165697

Chicago/Turabian Style

Wong, Daisy L., Amelia T. Yuan, Natalie C. Korkola, and Martin J. Stillman 2020. "Interplay between Carbonic Anhydrases and Metallothioneins: Structural Control of Metalation" International Journal of Molecular Sciences 21, no. 16: 5697. https://doi.org/10.3390/ijms21165697

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