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Article

Characterization and Comparison of Two Complete Plastomes of Rosaceae Species (Potentilla dickinsii var. glabrata and Spiraea insularis) Endemic to Ulleung Island, Korea

by
JiYoung Yang
1,
Gi-Ho Kang
2,
Jae-Hong Pak
1,* and
Seung-Chul Kim
3,*
1
Research Institute for Dok-do and Ulleung-do Island, Department of Biology, School of Life Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, Gyeongsangbuk-do 41566, Korea
2
Baekdudaegan National Arboretum, 1501 Chunyang-ro, Chungyang-myeon, Bonghwa-gun, Gyeongsangbuk-do 36209, Korea
3
Department of Biological Sciences, Sungkyunkwan University, 2066 Seobu-ro, Suwon, Gyeonggi-do 16419, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(14), 4933; https://doi.org/10.3390/ijms21144933
Submission received: 2 June 2020 / Revised: 2 July 2020 / Accepted: 10 July 2020 / Published: 13 July 2020
(This article belongs to the Section Molecular Genetics and Genomics)
Browse Figures
Versions Notes

Abstract

:
Potentilla dickinsii var. glabrata and Spiraea insularis in the family Rosaceae are species endemic to Ulleung Island, Korea, the latter of which is listed as endangered. In this study, we characterized the complete plastomes of these two species and compared these with previously reported plastomes of other Ulleung Island endemic species of Rosaceae (Cotoneaster wilsonii, Prunus takesimensis, Rubus takesimensis, and Sorbus ulleungensis). The highly conserved complete plastomes of P. dickinsii var. glabrata and S. insularis are 158,637 and 155,524 base pairs with GC contents of 37% and 36.9%, respectively. Comparative phylogenomic analysis identified three highly variable intergenic regions (trnT-UGU/trnL-UAA, rpl32/trnL-UAG, and ndhF/rpl32) and one variable genic region (ycf1). Only 6 of the 75 protein-coding genes have been subject to strong positive selection. Phylogenetic analysis of 23 representative plastomes within the Rosaceae supported the monophyly of Potentilla and the sister relationship between Potentilla and Fragaria and indicated that S. insularis is sister to a clade containing Cotoneaster, Malus, Pyrus, and Sorbus. The plastome resources generated in this study will contribute to elucidating the plastome evolution of insular endemic Rosaceae on Ulleung Island and also in assessing the genetic consequences of anagenetic speciation for various endemic lineages on the island.

1. Introduction

The family Rosaceae (Rosid I, Fabidae) comprises approximately 3000 species in 91 genera within three subfamilies (Rosoideae, Amygdaloideae, and Dryadoideae) and includes numerous economically important commercial fruit species, such as Malus (apples), Prunus (almonds, plums, peaches, cherries, etc.), Pyrus (pears), Rubus (raspberries, blackberries), and Fragaria (strawberries) [1,2,3]. Numerous ornamental woody species, such as crabapples (Malus), hawthorns (Crataegus), roses (Rosa), and rowans (Sorbus), are also valued members of this family. In addition to being a source of important commercial fruit and ornamental/landscaping trees, woody species of Rosaceae provide important habitats and food resources for innumerable forest animals, including birds and mammals [2]. The family Rosaceae is particularly diverse in temperate regions of the Northern Hemisphere and in this regard, it has been suggested that several closely related factors, including hybridization, pseudogamous gametophytic apomixes, polyploidy, and self-compatibility, have been driving forces that have contributed to promoting increases in the number of species in certain groups [1,4,5,6]. Given its considerable economic and taxonomic significance, the family Rosaceae has been subject to numerous genomic and phylogenetic studies [1,6,7,8,9,10]. Among the numerous important contributions to the phylogenetic characterization of Rosaceae, the whole plastid phylogenomic approach, based on 142 accessions, representing 87 genera, reported by Zhang et al., is of particular interest because they attempted to reconstruct deep relationships and reveal temporal diversification of the family [10]. In addition, on the basis of 125 new transcriptomic and genomic datasets, Xiang et al. fully resolved the phylogeny of Rosaceae, revealing numerous whole-genome duplications and providing a foundation for understanding the evolution of fruit-bearing plants in the Rosaceae [6].
Ulleung Island is located approximately 137 km off the east coast of the Korean Peninsula and approximately 300 km west of the Japanese archipelago. The island is estimated to be approximately 1.8 million years old and has a total area of 73 km2 [11]. Ulleung Island is home to some 500 native vascular plants species, of which approximately 40 (nearly 8%) are endemic, and is renowned for the exceptionally high number of plant species that evolved via anagenetic speciation [12,13]. Thus, the endemic plants of Ulleung Island represent an ideal model system for investigating the mechanism of anagenetic speciation and evaluating its genetic consequences [14,15,16,17,18,19,20]. Of the nearly 40 endemic species inhabiting Ulleung Island, Rosaceae includes the largest number of diverse endemics. In addition to having autapomorphies for each species, these Ulleung endemics show a general trend toward increasing leaf and flower size and loss of spines, which may be related to an absence of herbivores on the island [12,15].
Spiraea insularis (=Physocarpus insularis), belongs to the subfamily Amygdaloideae and is found rarely in the southeastern part of Ulleung Island [1,21,22,23,24] (Figure 1). Given its extreme rarity and highly restricted distribution, S. insularis is categorized and protected as a critically endangered species [CRB2ab(ii)] by the Korean government [25]. The classification and taxonomic position of this species has continued to prove problematic. For example, Kim et al. [26] treated it as a synonym of Spiraea chamaedryfolia var. ulmifolia, but various floristic studies have all identified P. insularis as a distinct species [27,28,29]. Given the established intercontinental disjunct distribution of Physocarpus [30] and that P. insularis is the only Physocarpus species native to Korea, its taxonomic status had remained uncertain until Oh et al. [31] confirmed its phylogenetic position within the genus Spiraea. Furthermore, on the basis of a few autapomorphic morphological characteristics, P. insularis was subsequently transferred and recognized as the new combination S. insularis (Nakai) H. Shin, Y.D. Kim and S.H. Oh [32]. The other Rosaceous endemic Potentilla dickinsii var. glabrata, a perennial herb, belongs to the subfamily Rosoideae sensu [1] (Figure 2). It was originally described as a new taxon by Nakai and is closely related to P. dickinsii var. dickinsii, which is distributed on the mainland of Korea and Japan [21]. However, Naruhashi [33] treated this taxon as a synonym of P. dickinsii var. dickinsii based on the wide infraspecific variation of P. dickinsii. Nevertheless, it has been suggested that P. dickinsii var. glabrata should be recognized as a distinct taxon based on its well-developed thick rhizomes and trifoliate basal leaves that differ from the typically pinnately compound leaves found in var. dickinsii [34]. It is widely acknowledged that species within the genus Potentilla are taxonomically a very challenging group, owing to hybridization, allopolyploidy, and apomixes [35,36,37].
The chloroplast genome can provide ample and vital information regarding genetic variation at the different taxonomic levels, particularly with respect maternal genetic characteristics, given its typical uniparental mode of inheritance in angiosperms [38]. Plastomes have accordingly been efficiently utilized to infer the phylogenetic relationships and evolutionary histories of numerous plant groups [10,39,40,41]. Since the first report of the complete plastome sequences of strawberry (Fragaria vesca) and apple (Malus × domestica), the plastome sequences of numerous species in the Rosaceae have been characterized over the past decade, which has been markedly facilitated by the advent of next-generation sequencing (NGS) technology [42,43,44,45,46,47,48,49]. Recently, complete plastome sequences have also been reported for several plant species among the diverse groups on Ulleung Island, including Campanula takesimana (Campanulaceae) [50], Chrysanthemum lucidum (Asteraceae) [51], Fagus multinervis (Fagaceae) [52], Lilium hansonii (Liliaceae) [53], Tilia insularis (Malvaceae) [54], Epilobium ulleungensis (Onagraceae) [55], Phytolacca insularis (Phytolaccaceae) [56], and Acer takesimense (Sapindaceae) [57]. Moreover, complete plastome sequences have been obtained for four species of Rosaceae endemic to Ulleung, namely, Rubus takesimensis, Prunus takesimensis, C. wilsonii, and Sorbus ulleungensis, which have provided important information regarding plastome organization and evolution [47,48,58,59].
In this study, we characterized the complete chloroplast genome sequences of Potentilla dickinsii var. glabrata (subfamily Rosoideae) and Spiraea insularis (subfamily Amygdaloideae), which are the only Rosaceae species endemic to Ulleung for which plastomes are yet to be sequenced, and compared these with the plastomes of the aforementioned four Ulleung Rosaceae species. The comparative analysis of these six plastomes will shed light on the plastome structure and evolution of endemic insular species in the family Rosaceae, which have evolved through the speciation mechanism of anagenesis. We anticipate that further analyses of these plastome sequences will enable us to identify hotspot regions that contribute to determining population genetic diversity and structure, thereby allowing us to assess genetic differences between pairs of continental progenitor and insular derivative species.

2. Results and Discussion

2.1. Genome Size and Features

The plastome of P. dickinsii var. glabrata has 155,524 bp and comprises a large single-copy (LSC) region of 85,213 bp, a small single-copy (SSC) region of 18,657 bp, and two inverted repeat (IR) regions of 25,827 bp. The complete plastome sequence of S. insularis is slightly larger at 158,637 bp and comprises an LSC region of 86,997 bp, an SSC region of 18,910 bp, and two IR regions of 26,365 bp (Figure 1 and Figure 2, and Table 1). The plastomes of P. dickinsii var. glabrata and S. insularis contain 131 and 132 genes, respectively, with the difference in gene number being attributable to the presence of an rps19 pseudogene in S. insularis. Both plastomes contain 84 protein-coding, eight ribosomal RNA, and 37 transfer RNA genes. The overall guanine–cytosine (GC) content of the P. dickinsii var. glabrata and S. insularis plastomes are 37.0% and 36.91%, respectively (Table 1). Of the six Rosaceae endemic to Ulleung Island, C. wilsonii is characterized by the longest plastome (159,997 bp), whereas that of P. dickinsii var. glabrata is the shortest. The plastomes of R. takesimensis and Sorbus ulleungensis were found to have the highest and lowest GC content of 37.1% and 36.5%, respectively. The plastome sequences of both P. dickinsii var. glabrata and S. insularis were found to contain a total of 17 duplicated genes in the IR regions (seven tRNA, four rRNA, and six protein-coding genes). Fifteen genes (ndhA, ndhB, petB, petD, rpl2, rpl16, rpoC1, rps12, rps16, trnA-UGC, trnG-UCC, trnI-GAU, trnK-UUU, trnL-UAA, and trnV-UAC) contain a single intron, whereas clpP and ycf3 each contain two introns.
In both P. dickinsii var. glabrata and S. insularis, the plastome contains a partial ycf1 gene of 1227 and 1301 bp, respectively, located in the IRb/SSC junction region, whereas a complete ycf1 gene of 5808 and 5613 bp, respectively, is located in the IR region at the SSC/IRa junction. The infA gene located in the LSC region of the P. dickinsii var. glabrata and S. insularis has become a pseudogene. Interestingly, the highly conserved group II intron of atpF has been lost in S. insularis, as has previously been observed in the Rubus species R. boninensis, R. crataegifolius, R. takesimensis, and R. trifidus [47,48,60]. When compared with representative plastomes of species in the Rosaceae, those of Potentilla, Fragaria, Rosa, and Rubus in the subfamily Rosoideae lineage all show atpF intron loss, whereas in contrast, the plastomes of Cotoneaster, Malus, Prunus, Pyrus, and Sorbus in the subfamily Amygdaloideae retain intron-containing atpF genes (Table 1). On the basis of the current phylogenetic framework, it appears that loss of the atpF intron has occurred only once in the subfamily Rosoideae; however, it remains to be determined whether this loss has also occurred in other lineages of Rosoideae, as well as in the broader phylogenetic framework, including within the Rosacea and Rosid families.
The frequency of codon usage in the P. dickinsii var. glabrata and S. insularis plastomes was calculated for the chloroplast genome based on the sequences of protein-coding and tRNA genes (Figure 3), which revealed that the average codon usage in these two species was nearly identical, i.e., 26,008 for P. dickinsii var. glabrata and 26,015 for S. insularis. Moreover, we found the distribution of codon types to be consistent. The relative synonymous codon usage (RSCU) value was also similar to that in R. takesimensis. Consistent with the patterns detected in Rubus [60] and other angiosperms [61] and algal lineages [62], we found that codon usage in the P. dickinsii var. glabrata and S. insularis plastomes is biased toward a high RSCU value of U and A at the third codon position.
The predicted number of RNA editing sites in the plastomes of P. dickinsii var. glabrata and S. insularis is 42 and 51, respectively, with the same cut-off value, and 19 and 15 of 35 protein-coding genes are predicted to undergo RNA editing, respectively (Table S1). These genes include photosynthesis-related genes (atpF, atpI, ndA, ndhB, ndhD, ndhF, ndhG, petB, petG, psbE, and psbF), self-replication genes (rpl23, rpoA, rpoB, rpoC2, rps2, rps14, and rps16), and others (accD, clpP, and matK). We detected no RNA editing sites in the accD, atpF, atpI, psbE, psbF, and rps16 genes of the P. dickinsii var. glabrata plastome, whereas no RNA editing sites were found at petG and rpl23 in the S. insularis plastome. Compared with other species, the ndhF gene of S. insularis showed an exceptionally high frequency (i.e., three-fold higher) of RNA editing sites. The ndhB gene is also characterized by the highest number of potential editing sites (11 sites), followed by the ndhD gene (6 sites), which is consistent with the findings of previous studies [63,64,65].

2.2. Comparative Analysis of Genome Structure

The plastomes of the six Rosaceae species endemic to Ulleung Island (i.e., C. wilsonii, S. insularis, P. dickinsii var. glabrata, Prunus takesimensis, R. takesimensis, and Sorbus ulleungensis) were plotted with mVISTA, using the annotated R. takesimensis plastome as a reference (Figure 4). The results indicated that the LSC region is the most divergent, whereas the two IR regions are highly conserved. In addition, the non-coding regions were found to be more divergent and variable than the coding regions. As expected, these findings are consistent with the patterns observed in common angiosperms [44,47,49,60,61]. These six plastomes are highly conserved despite differences in estimated divergence times in the Late Cretaceous period, with the crown ages of Rosoideae and Amygdaloideae being estimated to be 75.78 million and 90.18 million years, respectively [10].
Sliding window analysis performed using the DnaSP program revealed highly variable regions in the plastomes of the six endemic Rosaceae taxa (Figure 5). Comparison of the six plastomes revealed that the average value of nucleotide diversity (Pi) over the entire chloroplast genome was 0.042, with the most variable region (a Pi value of 0.14508) being the trnT-UGU/trnL-UAA intergenic region. We also detected high variability in two other intergenic regions (rpl32/trnL-UAG (Pi = 0.14342) and ndhF/rpl32 (Pi = 0.13267)) and one genic region (ycf1 (Pi = 0.1285)). In addition, we detected several variable regions with Pi values greater than 0.1, namely, rps16/trnQ-UUG, trnR-UCU/atpA, rpoB/trnC-GCA/petN, trnT-GGU/psbD, trnP-UGG/psaJ/rpl33, and ycf3/trnS-GGA/rps4. Among these highly variable regions, those with Pi values greater than 0.12 can be used to generate chlorotype diversity data to infer the origin and evolution of endemic species on Ulleung Island. Although the two newly sequenced species are only rarely found on Ulleung Island, R takesimensis is among the more commonly occurring species on the island, and we similarly detected highly variable regions, including rpl32/trnL, rps4/trnT, trnT/trnL, and psbZ/trnG, in this species [48]. Furthermore, we also found that the ycf1 gene shows the highest sequence divergence and, thus, would appear to have potential value for the phylogenetic analysis of Rosaceae and angiosperms in general [46].
Positive selection analysis, performed using the EasyCodeML [66] program with the site-specific model based on CodeML algorithms [67], enabled us to identify positively selected genes among endemic Rosaceae on Ulleung Island (Table 2). Among the conserved genes, six genes with positively selected sites within the endemic Rosaceae plastomes on Ulleung Island were identified with effectively significant LRT p values (Table 2). These six genes include one subunit of acetyl-CoA carboxylase (accD), one Rubisco gene (rbcL), one ribosome small subunit gene (rps3) of self-replication, and three NADH-dehydrogenase subunit genes (ndhB, nhdD, and ndhF) of photosynthesis. Based on the M8 model, the rbcL gene had five positive sites, followed by ndhF (three sites), and rps3 (two sites). The other three genes each had only one positive site. However, most of the genes, 69 of the 75 genes, had an average Ka/Ks ratio of below 1, indicating that these genes have been subjected to strong purifying selection in the Rosaceae chloroplast. In general, previous studies showed that Ka/Ks values are usually less than one [68], because synonymous nucleotide substitutions occur more frequently than nonsynonymous substitutions. Additionally, most genes of the chloroplast genome evolved under purifying selection due to functional limitation during chloroplast genome evolution [69,70,71,72]. Furthermore, both the positive selection of the rbcL gene and the NADH dehydrogenase subunit genes were previously reported in several studies, which is related to temperature, drought, carbon dioxide concentration, and photosynthetic rate [71,72,73,74]. Positive selection is considered to be indicative of an adaptation to environmental change, ecological niche, or coevolutionary processes [73,75], and we can, thus, speculate that the selection patterns detected for the Rosaceae endemic taxa on Ulleung Island may be associated with adaptation to an oceanic climate in the insular setting. However, any correlation between insular environment and positive selection pressures on genes will require further study.

2.3. Phylogenetic Analysis

Maximum likelihood analysis conducted on the best-fit model of “K3Pu + F + G4” enabled us to reveal phylogenetic positions among the endemic Rosaceae taxa on Ulleung Island (Figure 6). However, given that the phylogenetic tree was constructed based on only a partial representation of the entire Rosaceae family, the positions determined should be considered provisional and interpreted with caution. Nevertheless, our phylogenetic analysis of 29 representative plastomes within the rose family strongly supports the monophyly of Potentilla (100% bootstrap support) and the sister relationship between Potentilla and Fragaria (100% bootstrap support). We found that P. dickinsii var. glabrata was sister to a clade containing P. freyniana, P. freyniana var. chejuensis, P. stolonifera, and P. stolonifera var. quelpaertensis, whereas the clade of genus Spiraea (S. insularis and S. martini) is sister to a clade containing Cotoneaster, Malus, Pyrus, and Sorbus (Amygdaloideae; 100% bootstrap support). In addition, the monophyly of Cotoneaster, Spiraea and Sorbus was strongly supported, with a 100% bootstrap support value of each genus. Cotoneaster wilsonii showed sister relationships with two congeneric species, C. horizontalis and C. franchetii, while Sorbus ulleungensis showed sister relationships with congeneric S. helenae and S. rufopilosa. Lastly, we found that the genus Prunus represented the earliest diverged lineage within the subfamily Amygdaloideae and the monophyly of Amygdaloideae and Rosoideae was strongly supported with 100% bootstrap support.

3. Materials and Methods

3.1. Plant Sampling, DNA Isolation, and Plastome Sequencing/Annotation

To characterize plastome sequences among endemic species of the family Rosaceae on Ulleung Island, we collected samples from Potentilla dickinsii var. glabrata and Spiraea insularis, the two Ulleung endemic species in this family for which the plastomes have yet to be sequenced. Fresh leaves were collected from the Key-Chungsan Botanical Garden, which was specifically designated by the Ministry of Environment, Korea for the ex situ conservation of numerous native and endemic plant species from Ulleung Island. Voucher specimens were collected and deposited in the Ha Eun Herbarium of Sungkyunkwan University, Korea. Total DNA was isolated using a DNeasy Plant Mini Kit (Qiagen, Carlsbad, CA, USA) and sequenced using an Illumina HiSeq 4000 sequencer (Illumina, Inc., San Diego, CA, USA) at Macrogen Corporation (Seoul, Korea). A total of 33,832,832 and 29,396,622 paired-end reads (150 bp) were generated for P. dickinsii var. glabrata and S. insularis, respectively, and these were subsequently assembled de novo using Velvet v. 1.2.10 (EMBL-EBI, Cambridge, UK) with multiple k-mers [76]. tRNAs in the sequences were confirmed using tRNAscan-SE 2.0 (The Lowe lab, Santa Cruz, CA, USA) [77]. Annotation was conducted using Geneious R10 (Biomatters, Auckland, NewZealand) [78] and the annotated plastome sequences were deposited in the GenBank databank (with accession numbers of MT412406 and MT412405 for P. dickinsii var. glabrata and S. insularis, respectively). The annotated GenBank (NCBI, Bethesda, MD, USA) format sequence file was used to draw a circular map with the OGDRAW program v1.2 (CHLOROBOX, Postdam-Golm, Germany) [79].

3.2. Comparative Plastome Analysis

The complete plastomes of P. dickinsii var. glabrata and S. insularis were compared with those previously obtained for four Rosaceae species endemic to Ulleung, namely, C. wilsonii (NC046834), Prunus takesimensis (NC039379), R. takesimensis (NC037991), and Sorbus ulleungensis (NC03702). The six Rosaceae plastomes were aligned using MAFFT v. 7 [80] and adjusted manually using Geneious [78]. Using DnaSP v. 6.10 software [81], we performed a sliding window analysis, with a step size of 200 bp and window length of 800 bp, to determine plastome nucleotide diversity (Pi). The codon usage frequency was calculated using MEGA7 [82], yielding RSCU values [83], which are a simple measure of the non-uniform usage of synonymous codons in a coding sequence. For this purpose, we employed the DNA code used by bacteria, archaea, prokaryotic viruses, and in plant chloroplasts [84]. To predict putative RNA editing sites in the six plastomes, protein-coding genes were identified using the online program predictive RNA editor for plants (PREP) suite [85], with 22 genes used as references, based on a cut-off value of 0.8. Analyses based on the complete chloroplast genomes and the concatenated sequences of 75 common protein-coding genes among the studied species were conducted with MAFFT v. 7 [80], using Geneious R10 [78], and the Maximum likelihood phylogenetic tree was constructed with IQ-TREE ver. 1.4.2 [86]. To evaluate for natural selection pressure in the protein coding genes of the six plastomes, the site-specific model was performed using EasyCodeML [66] with CODEML algorithms [67]. Seven codon substitution models were investigated and compared to detect positively selected sites based on likelihood ratio tests (M0, M1a, M2A, M3, M7, M8, and M8a).

3.3. Phylogenetic Analysis

For the purposes of phylogenetic analysis, we analyzed the complete plastome sequences of 23 representative species from the family Rosaceae: seven species of Potentilla, including P. centigrana (NC041209), P. freyniana (NC041210), and P. stolonifera (NC044418); two species each from the genera Fragaria, Malus, Prunus, Pyrus, Rosa, and Rubus; one species each from the genera Cotoneaster, Physocarpus, and Sorbus. Dryas drummondii of the subfamily Dryadoideae was included as an outgroup species. The sequences of all species were aligned using MAFFT v. 7 [80] in Geneious [78]. Maximum likelihood analysis based on the best-fit model of K3Pu + F + G4 was conducted using IQ-TREE v. 1.4.2 [86], and non-parametric bootstrap analysis was performed with 1000 replicates.

4. Conclusions

In this study, we determined the complete plastome sequences of two species in the family Rosaceae (Potentilla dickinsii var. glabrata and Spiraea insularis) that are endemic to Ulleung Island, Korea. We found very little structural or organizational differences among the plastomes of six Rosaceae species endemic to this island. The frequency of codon usage was biased toward high RSCU values of U and A at the third codon position, and we found that the ndhB and ndhD genes are characterized by a high number of potential RNA editing sites. Comparative analysis among the six endemic Rosaceae species revealed three highly variable intergenic regions (trnT-UGU/trnL-UAA, rpl32/trnL-UAG, and ndhF/rpl32) and a single highly variable genic region (ycf1). These hotspot regions could be used to assess the genetic consequences of anagenetic speciation for endemic Rosaceae taxa on Ulleung Island. We also confirmed that a majority of the protein-coding genes (61 of 75) common to the chloroplast genomes of the endemic Rosaceae have been subjected to positive selection. Phylogenomic analysis based on selected Rosaceae plastomes supported the monophyly of the genus Potentilla and the sister relationship between S. insularis and a clade containing Cotoneaster, Malus, Pyrus, and Sorbus. The plastome resources reported in this study will enable us to gain a better understanding of the plastome evolution of insular endemics among the Rosaceae on Ulleung Island, as well as that of other members within the family Rosaceae.

Supplementary Materials

Supplementary materials can be found at https://www.mdpi.com/1422-0067/21/14/4933/s1. Table S1. Predicted RNA editing sites in the complete chloroplast genomes of two Rosaceae species endemic to Ulleung Island.

Author Contributions

S.-C.K. and J.Y. conceived and designed the experiments. J.Y. and G.-H.K. collected the samples and J.Y. generated and analyzed the data. J.Y. wrote the draft of the manuscript and S.-C.K. and J.-H.P. revised it. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), which is funded by the Ministry of Education (2016R1A6A1A0511910 and 2019R1A2C1011221), to J.-H.P. and J.Y., respectively.

Acknowledgments

We thank the officials of the Key-Chungsan Botanical garden (the director, Lee Sam-Woo and team manager, Kim Yoon-Young) for providing plant materials, and Yoon J.-S. (KNU) and Kim S.-H. (SKK) for field assistance. We also thank Jin-Oh Hyun for providing a photograph of Potentilla dickinsii var. glabrata taken on Ulleung Island.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Potter, D.; Eriksson, T.; Evans, R.C.; Oh, S.; Smedmark, J.; Morgan, D.R.; Kerr, M.; Robertson, K.R.; Arsenaut, M.; Dickinson, T.A.; et al. Phylogeney and classification of Rosaceae. Plant Syst. Evol. 2007, 266, 5–43. [Google Scholar] [CrossRef]
  2. Hummer, K.E.; Janick, J. Rosaceae: Taxonomy, economic importance, genomics. In Genetics and Genomics of Rosaceae; Folta, K.M., Gardiner, S.E., Eds.; Springer: New York, NY, USA, 2009; pp. 1–17. [Google Scholar]
  3. Phipps, J.B. Magnoliophyta: Picramniaceae to Rosaceae. In Flora of North America North of Mexico; Oxford University Press: New York, NY, USA; Oxford, UK, 2014; Volume 9. [Google Scholar]
  4. Vamosi, J.C.; Dickinson, T.A. Polyploidy and diversification: A phylogenetic investigation in Rosaceae. Int. J. Plant Sci. 2006, 167, 349–358. [Google Scholar] [CrossRef] [Green Version]
  5. Dickinson, T.A.; Lo, E.; Talent, N. Polyploidy, reproductive biology, and Rosaceae: Understanding evolution and making classifications. Plant Syst. Evol. 2007, 266, 59–78. [Google Scholar] [CrossRef]
  6. Xiang, Y.; Huang, C.-H.; Hu, Y.; Wen, J.; Li, S.; Yi, T.; Chen, H.; Xiang, J.; Ma, H. Evolution of Rosaceae fruit types based on nuclear phylogeny in the context of geological times and genome duplication. Mol. Biol. Evol. 2016, 34, 262–281. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  7. Nikiforova, S.V.; Cavalieri, D.; Velasco, R.; Goremykin, V. Phylogenetic Analysis of 47 Chloroplast Genomes Clarifies the Contribution of Wild Species to the Domesticated Apple Maternal Line. Mol. Biol. Evol. 2013, 30, 1751–1760. [Google Scholar] [CrossRef] [Green Version]
  8. Wu, J.; Wang, Z.; Shi, Z.; Zhang, S.; Ming, R.; Zhu, S.; Khan, M.A.; Tao, S.; Korban, S.S.; Wang, H.; et al. The genome of the pear (Pyrus bretschneideri Rehd.). Genome Res. 2013, 23, 396–408. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  9. Chin, S.W.; Shaw, J.; Haberle, R.; Wen, J.; Potter, D. Diversification of almonds, peaches, plums and cherries-molecular systematics and biogeographic history of Prunus (Rosaceae). Mol. Phylogentics Evol. 2014, 76, 34–48. [Google Scholar] [CrossRef]
  10. Zhang, S.-D.; Jin, J.J.; Chen, S.Y.; Chase, M.W.; Soltis, D.E.; Li, H.T.; Yang, J.B.; Li, D.Z.; Yi, T.S. Diversification of Rosaceae since the late Craetaceous based on plastid phylogenomics. New Phytol. 2017, 214, 1355–1367. [Google Scholar] [CrossRef] [Green Version]
  11. Kim, Y.K. Petrology of Ulreung volcanic island, Korea–part 1. Geology. J. Japan. AssocC Mineral Petrol Econ. Geol. 1985, 80, 128–135. [Google Scholar] [CrossRef]
  12. Sun, B.Y.; Stuessy, T.F. Preliminary observations on the evolution of endemic angiosperms of Ullung Island, Korea. In Evolution and Speciation of Island Plants; Stuessy, T.F., Ono, M., Eds.; Cambridge University Press: New York, NY, USA, 1998; pp. 181–202. [Google Scholar]
  13. Sun, B.-Y.; Shin, H.; Hyun, J.-O.; Kim, Y.-D.; Oh, S.-H. Vascular Plants of Dokdo and Ulleungdo Islands in Korea; The National Institute of Biological Resources, GeoBook Publishing Co.: Seoul, Korea, 2014. [Google Scholar]
  14. Stuessy, T.F.; Jakubowsky, G.; Gómez, R.S.; Pfosser, M.; Schlüter, P.M.; Fer, T.; Sun, B.-Y.; Kato, H. Anagenetic evolution in island plants. J. Biogeogr. 2006, 33, 1259–1265. [Google Scholar] [CrossRef]
  15. Givnish, T.J.; Sytsma, K.J.; Smith, J.F.; Hahn, W.J. Thorn-like prickles and heterophylly in Cyanea: Adaptations to extinct avian browsers on Hawaii? Proc. Natl. Acad. Sci. USA 1994, 91, 2810–2814. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Pfosser, M.; Jakubowsky, G.; Schluter, P.M.; Fer, T.; Kato, H.; Stuessy, T.F.; Sun, B.Y. Evolution of Dystaenia takesimana (Apiaceae), endemic to Ullung Island, Korea. Plant Syst. Evol. 2006, 256, 159–170. [Google Scholar] [CrossRef]
  17. Pfosser, M.; Guzy-Wrobelska, J.; Sun, B.Y.; Stuessy, T.F.; Sugawara, T.; Fujii, N. The origin of species of Acer (Sapindaceae) endemic to Ullung Island, Korea. Syst. Bot. 2002, 27, 351–367. [Google Scholar]
  18. Takayama, K.; Sun, B.Y.; Stuessy, T.F. Genetic consequences of anagenetic speciation in Acer okamotoanum (Sapindaceae) on Ullung Island, Korea. Ann. Bot. 2012, 109, 321–330. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Takayama, K.; Sun, B.Y.; Stuessy, T.F. Anagenetic speciation in Ullung Island, Korea: Genetic diversity and structure in the island endemic species, Acer takesimense (Sapindaceae). J. Plant Res. 2013, 126, 323–333. [Google Scholar] [CrossRef] [Green Version]
  20. Yang, J.Y.; Pak, J.-H.; Maki, M.; Kim, S.-C. Multiple origins and the population genetic structure of Rubus takesimensis (Rosaceae) on Ulleung Island: Implications for the genetic consequences of anagenetic speciation. PLoS ONE 2019, 14, e0222707. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  21. Nakai, T. Notulae ad plantas Japoniae et Koreae XVII. Bot. Mag. Tokyo 1918, 32, 103–110. [Google Scholar] [CrossRef] [Green Version]
  22. Nakai, T. Report on the Vegetation of the Island Ooryongto or Dagelet Island; The Government of Chosen: Seoul, Korea, 1919.
  23. Oh, S.Y. Floral studies on the vascular plants of the Dagelet Island. Res. Rev. Kyungpook Natl. Univ. 1978, 25, 131–201. [Google Scholar]
  24. Lee, W.T.; Yang, I.S. The flora of Ulreung Is. and Dogdo Island. Rep. KACN 1981, 19, 61–95. [Google Scholar]
  25. National Institute of Biological Resources. Spiraea insularis. In Korean Red List of Threatened Species, 2nd ed.; Suh, M.-H., Lee, B.-Y., Kim, S.T., Park, C.-H., Oh, H.-K., Kim, H.-Y., Lee, J.-H., Lee, S.Y., Eds.; Ministry of environment: Incheon, Korea, 2014; p. 141. [Google Scholar]
  26. Kim, C.H.; Kim, T.J.; Sun, B. Taxonomic identities of some endemic Korean vascular plants. Korean J. Plant Taxon. 2000, 30, 355–361. [Google Scholar] [CrossRef]
  27. Chung, T.H. Korean Flora; Shinjisa: Seoul, Korea, 1957. [Google Scholar]
  28. Lee, S. Physocarpus. In Genera of Vascular Plants of Korea; Park, C.W., Ed.; Academy: Seoul, Korea, 2007; pp. 538–539. [Google Scholar]
  29. Lee, B.C. Rare Plants Data Book in Korea; Korea National Arboretum: Pocheon, Korea, 2008. [Google Scholar]
  30. Oh, S.H.; Potter, D. Molecular phylogenetic systematics and biogeography of tribe Neillieae (Rosaceae) using DNA sequences of cpDNA, rDNA, and LEAFY. Am. J. Bot. 2005, 92, 179–192. [Google Scholar] [CrossRef]
  31. Oh, S.-H.; Chen, L.; Kim, S.-H.; Kim, Y.-D.; Shin, H.C. Phylogenetic Relationship of Physocarpus insularis (Rosaceae) Endemic on Ulleung Island: Implications for Conservation Biology. J. Plant Biol. 2010, 53, 94–105. [Google Scholar] [CrossRef]
  32. Shin, H.C.; Kim, Y.-D.; Oh, S.-H. A New Combination in Spiraea (Rosaceae) from Ulleung Island, Korea. Novon 2011, 21, 373–374. [Google Scholar] [CrossRef]
  33. Naruhashi, N. Rosoideae. In Flora of Japan, Vol. IIb; Iwatsuki, K., Boufford, D.E., Ohba, H., Eds.; Kodansha: Tokyo, Japan, 2003; pp. 145–212. [Google Scholar]
  34. Heo, K.-I.; Lee, S.R.; Kim, Y.S.; Park, J.S.; Lee, S.T. Taxonomic studies of the tribe Potentilleae (Rosaceae) in Korea. Korean J. Plant Taxon. 2019, 49, 28–69. [Google Scholar] [CrossRef] [Green Version]
  35. Asker, S. Studies in Apomictic and Sexual Potentilla L.; Berlingska Boktrycheriet: Lund, Denmark, 1971; pp. 2–7. [Google Scholar]
  36. Soják, J. Notes on Potentilla I. Hybridogenous species derived from intersectional hybrids of sect. Niveae× sect. Multifidae. Bot. Jahrb. Syst. 1986, 106, 145–210. [Google Scholar]
  37. Eriksen, B. Mating systems in two species of Potentilla from Alaska. Folia Geobot. Phytotax. 1996, 31, 333–344. [Google Scholar] [CrossRef]
  38. Bock, R. Structure, function, and inheritance of plastid genomes. In Cell and Molecular Biology of Plastids; Bock, R., Ed.; Topics in Current Genetics; Springer: Berlin, Germany, 2007; Volume 19, pp. 29–63. [Google Scholar]
  39. Jansen, R.K.; Cai, Z.; Raubeson, L.A.; Daniell, H.; dePamphilis, C.W.; Leebens-Mack, J.; Müller, K.F.; Guisinger-Bellian, M.; Haberle, R.C.; Hansen, A.K.; et al. Analysis of 81 genes from 64 plastid genomes resolves relationships in angiosperms and identifies genome-scale evolutionary patterns. Proc. Natl. Acad. Sci. USA 2007, 104, 19369–19374. [Google Scholar] [CrossRef] [Green Version]
  40. Li, X.Q.; Zuo, Y.J.; Zhu, X.X.; Liao, S.; Ma, J.S. Complete chloroplast genomes and comparative analysis of sequences evolution among seven Aristolochia (Aristolochiaceae) medicinal species. Int. J. Mol. Sci. 2019, 20, 1045. [Google Scholar] [CrossRef] [Green Version]
  41. Cho, M.-S.; Yang, J.Y.; Yang, T.-J.; Kim, S.-C. Evolutionary comparison of the chloroplast genome in the woody Sonchus alliance (Asteraceae) on the Canary Islands. Genes 2019, 10, 217. [Google Scholar] [CrossRef] [Green Version]
  42. Shulaev, V.; Sargent, D.J.; Crowhurst, R.N.; Mockler, T.C.; Folkerts, O.; Delcher, A.L.; Jaiswal, P.; Mockaitis, K.; Liston, A.; Mane, S.P.; et al. The genome of woodland strawberry (Fragaria vesca). Nat. Genet. 2010, 43, 109–116. [Google Scholar] [CrossRef]
  43. Velasco, R.; Zharkikh, A.; Affourtit, J.; Dhingra, A.; Cestaro, A.; Kalyanraman, A.; Fontana, P.; Bhatnagar, S.K.; Troggio, M.; Pruss, D.; et al. The genome of the domesticated apple (Malus × domestica Borkh.). Nat. Genet. 2010, 42, 833–839. [Google Scholar] [CrossRef] [PubMed]
  44. Jansen, R.K.; Saski, C.; Lee, S.B.; Hansen, A.K.; Daniell, H. Complete plastid genome sequences of three rosids (Castanea, Prunus, Theobroma): Evidence for at least two independent transfers of rpl22 to the nucleus. Mol. Biol. Evol. 2011, 28, 835–847. [Google Scholar] [CrossRef] [Green Version]
  45. Terakami, S.; Matsumura, Y.; Kurita, K.; Kanamori, H.; Katayose, Y.; Yamamoto, T.; Katayama, H. Complete sequence of the chloroplast genome from pear (Pyrus pyrifoliae): Genome structure and comparative analysis. Tree Genet. Genomes 2012, 8, 841–854. [Google Scholar] [CrossRef]
  46. Yang, J.Y.; Pak, J.-H.; Kim, S.-C. The complete chloroplast genome sequence of Korean raspberry Rubus crataegifolius (Rosaceae). Mitochondrial DNA B 2017, 2, 793–794. [Google Scholar] [CrossRef] [Green Version]
  47. Yang, J.Y.; Pak, J.-H.; Kim, S.-C. The complete plastome sequence of Rubus takesimensis endemic to Ulleung Island, Korea: Insights into molecular evolution of anagenetically derived species in Rubus (Rosaceae). Gene 2018, 668, 221–228. [Google Scholar] [CrossRef] [PubMed]
  48. Yang, J.Y.; Pak, J.-H.; Kim, S.-C. Chloroplast genome of critically endangered Cotoneaster wilsonii (Rosaceae) endemic to Ulleung Island, Korea. Mitochondrial DNA B 2019, 4, 3892–3893. [Google Scholar] [CrossRef] [Green Version]
  49. Jeon, J.H.; Kim, S.-C. Comparative analysis of the complete chloroplast genome sequences of three closely related East-Asian wild Roses (Rosa sect. Synstylae; Rosaceae). Genes 2019, 10, 23. [Google Scholar] [CrossRef] [Green Version]
  50. Cheon, K.S.; Kim, K.A.; Jang, S.K.; Yoo, K.O. Complete chloroplast genome sequence of Campanula takesimana (Campanulaceae), an endemic to Korea. Mitochondrial DNA B 2014, 1, 184–185. [Google Scholar] [CrossRef]
  51. Kim, J.S.; Lee, W.; Pak, J.-H. The complete plastid genome sequence of Chrysanthemum lucidum (Asteraceae): An endemic species of Ulleung Island of Korea. Mitochondrial DNA B 2018, 3, 476–477. [Google Scholar] [CrossRef] [Green Version]
  52. Park, J.-S.; Jin, D.-P.; Park, J.-W.; Choi, B.-H. Complete chloroplast genome of Fagus multinervis, a beech species endemic to Ulleung Island in South Korea. Mitochondrial DNA B 2019, 4, 1698–1699. [Google Scholar] [CrossRef] [Green Version]
  53. Kim, K.H.; Hwang, Y.-J.; Lee, S.-C.; Yang, T.-J.; Lim, K.-B. The complete chloroplast genome sequence of Lilium hansonii Leichtlin ex D. D. T. Moore. Mitochondrial DNA A 2016, 27, 3678–3679. [Google Scholar] [CrossRef] [PubMed]
  54. Yang, J.Y.; Lee, W.; Pak, J.-H.; Kim, S.-C. Complete chloroplast genome of Ulleung Island endemic basswood, Tilia insularis (Malvaceae) in Korea. Mitochondrial DNA B 2018, 3, 605–606. [Google Scholar] [CrossRef] [Green Version]
  55. Yang, J.Y.; Chung, J.-M.; Kim, S.-C. Complete chloroplast genome of Ulleung Island endemic, Epilobium ulleungensis (Onagraceae), in Korea. Mitochondrial DNA B 2018, 3, 703–704. [Google Scholar] [CrossRef] [Green Version]
  56. Yang, J.Y.; Lee, W.; Pak, J.-H.; Kim, S.-C. Complete chloroplast genome of Ulleung Island endemic pokeweed, Phytolacca insularis (Phytolaccaceae), in Korea. Mitochondrial DNA B 2019, 4, 8–9. [Google Scholar] [CrossRef]
  57. Kim, H.T.; Pak, J.-H.; Kim, J.S. The complete chloroplast genome sequence of Acer takesimense (Sapindaceae), an endemic to Ullenung Island of Korea. Mitochondrial DNA B 2019, 4, 1531–1532. [Google Scholar] [CrossRef] [Green Version]
  58. Cho, M.-S.; Yang, J.Y.; Kim, S.-C. Complete chloroplast genome of Ulleung Island endemic flowering cherry, Prunus takesimensis (Rosaceae), in Korea. Mitochondrial DNA B 2018, 3, 274–275. [Google Scholar] [CrossRef]
  59. Gil, H.-Y.; Kim, S.-C. The plastome sequence of Ulleung Rowan, Sorbus ulleungensis (Rosaceae), a new endemic species on Ulleung Island, Korea. Mitochondrial DNA B 2018, 3, 284–285. [Google Scholar] [CrossRef]
  60. Yang, J.Y.; Takayama, K.; Pak, J.-H.; Kim, S.-C. Comparison of the Whole-Plastome Sequence between the Bonin Islands Endemic Rubus boninensis and Its Close Relative, Rubus trifidus (Rosaceae), in the Southern Korean Peninsula. Genes 2019, 10, 774. [Google Scholar] [CrossRef] [Green Version]
  61. Ravi, V.; Khurana, J.P.; Tyagi, A.K.; Khurana, P. An update on chloroplast genomes. Plant Syst. Evol. 2008, 271, 101–122. [Google Scholar] [CrossRef]
  62. Morton, B.R. Selection on the codon bias of chloroplast and cyanelle genes in different plant and algal lineages. J. Mol. Evol. 1998, 46, 449–459. [Google Scholar] [CrossRef]
  63. Rabah, S.O.; Lee, C.; Hajrah, N.H.; Makki, R.M.; Alharby, H.F.; Alhebshi, A.M.; Sabir, J.; Jansen, R.K.; Ruhlman, T.A. Plastome sequencing of ten nonmodel crop species uncovers a large insertion of mitochondrial DNA in cashew. Plant Genome 2017, 10, 1–14. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Pinard, D.; Myburg, A.A.; Mizrachi, E. The plastid and mitochondrial genomes of Eucalyptus grandis. BMC Genom. 2019, 20, 132. [Google Scholar] [CrossRef] [PubMed]
  65. Kim, S.-H.; Yang, J.Y.; Park, J.S.; Yamada, T.; Maki, M.; Kim, S.-C. Comparison of Whole Plastome Sequences between Thermogenic Skunk Cabbage Symplocarpus renifolius and Nonthermogenic S. nipponicus (Orontioideae; Araceae) in East Asia. Int. J. Mol. Sci. 2019, 20, 4678. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  66. Gao, F.; Chen, C.; Arab, D.A.; Du, Z.; He, Y.; Ho, S.Y.W. EasyCodeML: A visual tool for analysis of selection using CodeML. Ecol. Evolut. 2019, 9, 3891–3898. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  67. Yang, Z. PAML: A program package for phylogenetic analysis by maximum likelihood. Bioinformatics 1997, 13, 555–556. [Google Scholar] [CrossRef] [PubMed]
  68. Makałowski, W.; Boguski, M.S.; Hughes, A.L.; Yeager, M. Synonymous and nonsynonymous substitution distances are correlated in mouse and rat genes. J. Mol. Evol. 1998, 47, 119–121. [Google Scholar] [CrossRef]
  69. Sloan, D.B.; Triant, D.A.; Forrester, N.J.; Bergner, L.M.; Wu, M.; Taylor, D.R. A recurring syndrome of accelerated plastid genome evolution in the angiosperm tribe Sileneae (Caryophyllaceae). Mol. Phylogenet. Evol. 2014, 72, 82–89. [Google Scholar] [CrossRef]
  70. Cheng, H.; Li, J.; Zhang, H.; Cai, B.; Gao, Z.; Qiao, Y.; Mi, L. The complete chloroplast genome sequence of strawberry (Fragaria×ananassa Duch.) and comparison with related species of Rosaceae. PeerJ 2017, 5, e3919. [Google Scholar] [CrossRef] [Green Version]
  71. Jiang, P.; Shi, F.X.; Li, M.R.; Liu, B.; Wen, J.; Xiao, H.X.; Li, L.F. Positive selection driving cytoplasmic genome evolution of the medicinally important ginseng plant genus Panax. Front. Plant Sci. 2018, 9, 359. [Google Scholar] [CrossRef]
  72. Li, P.; Lou, G.; Cai, X.; Zhang, B.; Cheng, Y.; Wang, H. Comparison of the complete plastomes and the phylogenetic analysis of Paulownia species. Sci. Rep. 2020, 10, 2225. [Google Scholar] [CrossRef] [Green Version]
  73. Piot, A.; Hackel, J.; Christin, P.A.; Besnard, G. One-third of the plastid genes evolved under positive selection in PACMAD grasses. Planta 2018, 247, 255–266. [Google Scholar] [CrossRef] [PubMed]
  74. Wang, L.; Zhang, H.; Jiang, M.; Chen, H.; Huang, L.; Liu, C. Complete plastome sequence of Iodes cirrhosa Turcz., the first in the Icacinaceae, comparative genomic analyses and possible split of Idoes species in response to climate changes. PeerJ 2019, 7, e6663. [Google Scholar] [CrossRef] [PubMed]
  75. Burri, R.; Salamin, N.; Studer, R.A.; Roulin, A.; Fumagalli, L. Adaptive divergence of ancient gene duplicates in the avian MHC class II beta. Mol. Biol. Evol. 2010, 27, 2360–2374. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  76. Zerbino, D.R.; Birney, E. Velvet: Algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008, 18, 821–829. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  77. Lowe, T.M.; Eddy, S.R. tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997, 25, 955–964. [Google Scholar] [CrossRef]
  78. Kearse, M.; Moir, R.; Wilson, A.; Stones-Havas, S.; Cheung, M.; Sturrock, S.; Buxton, S.; Cooper, A.; Markowitz, S.; Duran, C.; et al. Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 2012, 28, 1647–1649. [Google Scholar] [CrossRef]
  79. Lohse, M.; Drechsel, O.; Bock, R. Organellar genome DRAW (OGDRAW): A tool for the easy generation of high-quality custom graphical maps of plastid and mitochondrial genomes. Curr. Genet. 2009, 25, 1451–1452. [Google Scholar]
  80. Katoh, K.; Standley, D.M. MAFFT multiple sequence alignment software v7: Improvements in performance and usability. Mol. Biol. Evol. 2013, 30, 772–780. [Google Scholar] [CrossRef] [Green Version]
  81. Rozas, J.; Ferrer-Mata, A.; Sánchez-DelBarrio, J.C.; Guirao-Rico, S.; Librado, P.; Ramos-Onsins, S.E.; Sánchez-Gracia, A. DnaSP v6: DNA sequence polymorphismanalysis of large datasets. Mol. Biol. Evol. 2017, 34, 3299–3302. [Google Scholar] [CrossRef]
  82. Kumar, S.; Stecher, G.; Tamura, K. MEGA7: Molecular evolutionary genetics analysis v7.0 for bigger datasets. Mol. Biol. Evol. 2016, 33, 1870–1874. [Google Scholar] [CrossRef] [Green Version]
  83. Sharp, P.M.; Li, W.H. An evolutionary perspective on synonymous codon usage in unicellular organisms. J. Mol. Evol. 1986, 24, 28–38. [Google Scholar] [CrossRef] [PubMed]
  84. Kozak, M. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol. Rev. 1983, 47, 1–45. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  85. Mower, J.P. The PREP suite: Predictive RNA editors for plant mitochondrial genes, chloroplast genes and user-defined alignments. Nucleic Acids Res. 2009, 37, W253–W259. [Google Scholar] [CrossRef] [PubMed]
  86. Nguyen, L.-T.; Schmidt, H.A.; von Haeseler, A.; Minh, B.Q. IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol. Biol. Evol. 2015, 32, 268–274. [Google Scholar] [CrossRef] [PubMed]
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Figure 1. The complete plastome map (a) and plant of Potentilla dickinsii var. glabrata (b). (Photograph credit: Jin-Oh Hyun, Northeastern Asia Biodiversity Institute, Korea).
Figure 1. The complete plastome map (a) and plant of Potentilla dickinsii var. glabrata (b). (Photograph credit: Jin-Oh Hyun, Northeastern Asia Biodiversity Institute, Korea).
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Figure 2. The complete plastome map (a) and plant of Spiraea insularis (b). (Photograph credit: Seung-Chul Kim, Sungkyunkwan University, Korea).
Figure 2. The complete plastome map (a) and plant of Spiraea insularis (b). (Photograph credit: Seung-Chul Kim, Sungkyunkwan University, Korea).
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Figure 3. Codon distribution and relative synonymous codon usage in the plastomes of two Rosaceae species endemic to Ulleung Island.
Figure 3. Codon distribution and relative synonymous codon usage in the plastomes of two Rosaceae species endemic to Ulleung Island.
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Figure 4. Visualization of alignment of the six plastome sequences of Rosaceae species endemic to Ulleung Island.
Figure 4. Visualization of alignment of the six plastome sequences of Rosaceae species endemic to Ulleung Island.
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Figure 5. Sliding window analysis of the six whole-chloroplast genomes of Rosaceae species endemic to Ulleung Island: 1—rps16/trnQ-UUG; 2—trnR-UCU/atpA; 3—rpoB/trnC-GCA/petN; 4—trnT-GGU/psbD; 5—ycf3/trn-GGA/rps4; and 6—trnP-UGG/psaJ/rpl33.
Figure 5. Sliding window analysis of the six whole-chloroplast genomes of Rosaceae species endemic to Ulleung Island: 1—rps16/trnQ-UUG; 2—trnR-UCU/atpA; 3—rpoB/trnC-GCA/petN; 4—trnT-GGU/psbD; 5—ycf3/trn-GGA/rps4; and 6—trnP-UGG/psaJ/rpl33.
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Figure 6. Maximum likelihood tree inferred from 29 representative taxa of Rosaceae. Bootstrap values based on 1000 replicates are shown on each node. Species indicated by red font are the six Rosaceae species endemic to Ulleung Island. * represent the newly assembled endemic plastomes of Rosaceae on Ulleung Island in this study.
Figure 6. Maximum likelihood tree inferred from 29 representative taxa of Rosaceae. Bootstrap values based on 1000 replicates are shown on each node. Species indicated by red font are the six Rosaceae species endemic to Ulleung Island. * represent the newly assembled endemic plastomes of Rosaceae on Ulleung Island in this study.
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Table
Table 1. Summary of the characteristics of the six endemic Rosaceae chloroplast genomes in Ulleung Island.
Table 1. Summary of the characteristics of the six endemic Rosaceae chloroplast genomes in Ulleung Island.
TaxaPotentilla dickinsii var.
glabrata		Spiraea
insularis		Cotoneaster
wilsonii		Prunus
takesimensis		Rubus
takesimensis		Sorbus
ulleungensis
Total cpDNA size (bp)155,524158,637159,999157,948155,760159,632
GC content (%)37.0%36.9%36.6%36.7%37.1%36.5%
LSC size (bp)/GC content (%)85,213/34.8%86,997/34.8%87,868/34.2%85,959/34.6%85,402/35.1%88,003/34.2%
IR size (bp)/GC content (%)25,827/42.8%26,365/42.5%26,399/42.6%26,436/42.5%25,781/42.8%26,402/42.6%
SSC size (bp)/GC content (%)18,657/30.7%18,910/30.5%19,335/30.3%19,117/30.2%18,750/31.1%18,824/30.5%
Number of genes131132 131131131131
Number of protein-coding genes848484848484
Number of tRNA genes373737373737
Number of rRNA genes888888
Number of duplicated genes171717171717
Number of single intron genes15 (intron loss in atpF)16161615 (intron loss in atpF)16
Number of two intron genes222222
Accession NumberMT412406MT412405NC046834NC039379NC037991NC03702
Table
Table 2. Log-likelihood values of the site-specific models, with detected sites having dN/dS values > 1.
Table 2. Log-likelihood values of the site-specific models, with detected sites having dN/dS values > 1.
Gene NameModels npln LLikelihood Ratio
Test p-Value		Positively Selected Sites
accDM814−3164.7339480.053131782134 M 0.971 *
M712−3167.668928
ndhBM814−2187.9546320.000001793371 S 0.974 *
M712−2201.186056
ndhDM814−2896.6053260.03100725742 I 0.959 *
M712−2900.078860
ndhFM814−4982.1468160.000002084643 S 0.952 *; 649 Q 0.990 *; 671 G 0.994 **
M712−4995.227947
rbcLM814−2701.3846530.00000000086 H 0.980 *; 142 T 0.990 **;
247 C 0.999 **; 279 S 0.990 **; 475 L 0.998 **
M712−2729.389370
rps3M814−1248.2021110.00094973397 T 0.952 *; 118 A 0.953 *
M712−1255.161441
* p < 0.05; ** p < 0.01. np represents the degree of freedom.

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Yang, J.; Kang, G.-H.; Pak, J.-H.; Kim, S.-C. Characterization and Comparison of Two Complete Plastomes of Rosaceae Species (Potentilla dickinsii var. glabrata and Spiraea insularis) Endemic to Ulleung Island, Korea. Int. J. Mol. Sci. 2020, 21, 4933. https://doi.org/10.3390/ijms21144933

AMA Style

Yang J, Kang G-H, Pak J-H, Kim S-C. Characterization and Comparison of Two Complete Plastomes of Rosaceae Species (Potentilla dickinsii var. glabrata and Spiraea insularis) Endemic to Ulleung Island, Korea. International Journal of Molecular Sciences. 2020; 21(14):4933. https://doi.org/10.3390/ijms21144933

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Yang, JiYoung, Gi-Ho Kang, Jae-Hong Pak, and Seung-Chul Kim. 2020. "Characterization and Comparison of Two Complete Plastomes of Rosaceae Species (Potentilla dickinsii var. glabrata and Spiraea insularis) Endemic to Ulleung Island, Korea" International Journal of Molecular Sciences 21, no. 14: 4933. https://doi.org/10.3390/ijms21144933

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