RIP1 Is a Novel Component of γ-ionizing Radiation-Induced Invasion of Non-Small Cell Lung Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. IR Induces Invasion/Migration via Upregulation of EMT and RIP1
2.2. IR-Induced Invasion/Migration Is Mediated by the EGFR/Src/STAT3 Pathway
2.3. Inhibition of RIP1 Protein Suppresses Invasion/Migration in IR-Treated A549 Cells
2.4. Knockdown of RIP1 Suppresses Invasion/Migration of IR-Treated A549 Cells
2.5. NF-κB Is Associated with RIP1 Expression and EMT in IR-Treated A549 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Chemical Reagents
4.2. Treatment with γ-IR
4.3. Propidium Iodide (PI) Uptake Assay
4.4. Migration and Invasion Assays
4.5. Immunoblot Analysis
4.6. Immunofluorescence (IF) Staining
4.7. Small Interference RNA and Transfection
4.8. Xenograft Tumorigenicity Assays and Immunohistochemistry
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
IR | γ-ionizing radiation |
RIP | Receptor-interacting protein |
MMP | Matrix metalloprotease |
EMT | Epithelial-mesenchymal transition |
EGFR | Epidermal growth factor receptor |
PI | Propidium iodide |
IF | Immunofluorescence |
SD | Standard deviation |
Nec | Necrostatin |
TME | Tumor microenvironment |
ECM | Extracellular matrix |
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Kang, A.-R.; Cho, J.H.; Lee, N.-G.; Song, J.-Y.; Hwang, S.-G.; Lee, D.-H.; Um, H.-D.; Park, J.K. RIP1 Is a Novel Component of γ-ionizing Radiation-Induced Invasion of Non-Small Cell Lung Cancer Cells. Int. J. Mol. Sci. 2020, 21, 4584. https://doi.org/10.3390/ijms21134584
Kang A-R, Cho JH, Lee N-G, Song J-Y, Hwang S-G, Lee D-H, Um H-D, Park JK. RIP1 Is a Novel Component of γ-ionizing Radiation-Induced Invasion of Non-Small Cell Lung Cancer Cells. International Journal of Molecular Sciences. 2020; 21(13):4584. https://doi.org/10.3390/ijms21134584
Chicago/Turabian StyleKang, A-Ram, Jeong Hyun Cho, Na-Gyeong Lee, Jie-Young Song, Sang-Gu Hwang, Dae-Hee Lee, Hong-Duck Um, and Jong Kuk Park. 2020. "RIP1 Is a Novel Component of γ-ionizing Radiation-Induced Invasion of Non-Small Cell Lung Cancer Cells" International Journal of Molecular Sciences 21, no. 13: 4584. https://doi.org/10.3390/ijms21134584