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Article

How Charge and Triple Size-Selective Membrane Separation of Peptides from Salmon Protein Hydrolysate Orientate their Biological Response on Glucose Uptake

1
Department of Food Sciences and Laboratory of Food Processing and Electromembrane Processes (LTAPEM), Université Laval, Quebec, QC G1V 0A6, Canada
2
Institute of Nutrition and Functional Foods (INAF), Université Laval, Quebec, QC G1V 0A6, Canada
3
Québec Heart and Lung Institute, Université Laval, Department of Medicine, Quebec, QC G1V 4G5, Canada
4
Department of Process Engineering and Applied Science, Dalhousie University, P.O. Box 15,000, Halifax, NS B3H 4R2, Canada
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(8), 1939; https://doi.org/10.3390/ijms20081939
Received: 22 March 2019 / Revised: 16 April 2019 / Accepted: 17 April 2019 / Published: 20 April 2019
(This article belongs to the Special Issue Ion and Molecule Transport in Membrane Systems)
The valorization of by-products from natural organic sources is an international priority to respond to environmental and economic challenges. In this context, electrodialysis with filtration membrane (EDFM), a green and ultra-selective process, was used to separate peptides from salmon frame protein hydrolysate. For the first time, the simultaneous separation of peptides by three ultrafiltration membranes of different molecular-weight exclusion limits (50, 20, and 5 kDa) stacked in an electrodialysis system, allowed for the generation of specific cationic and anionic fractions with different molecular weight profiles and bioactivity responses. Significant decreases in peptide recovery, yield, and molecular weight (MW) range were observed in the recovery compartments depending on whether peptides had to cross one, two, or three ultrafiltration membranes. Moreover, the Cationic Recovery Compartment 1 fraction demonstrated the highest increase (42%) in glucose uptake on L6 muscle cells. While, in the anionic configuration, both Anionic Recovery Compartment 2 and Anionic Recovery Compartment 3 fractions presented a glucose uptake response in basal condition similar to the insulin control. Furthermore, Cationic Recovery Compartment 3 was found to contain inhibitory peptides. Finally, LC-MS analyses of the bioassay-guided bioactive fractions allowed us to identify 11 peptides from salmon by-products that are potentially responsible for the glucose uptake improvement. View Full-Text
Keywords: electrodialysis with filtration membrane (EDFM); triple size-selective separation; glucose uptake; bioassay-guided validation; bioactive peptides electrodialysis with filtration membrane (EDFM); triple size-selective separation; glucose uptake; bioassay-guided validation; bioactive peptides
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MDPI and ACS Style

Henaux, L.; Thibodeau, J.; Pilon, G.; Gill, T.; Marette, A.; Bazinet, L. How Charge and Triple Size-Selective Membrane Separation of Peptides from Salmon Protein Hydrolysate Orientate their Biological Response on Glucose Uptake. Int. J. Mol. Sci. 2019, 20, 1939. https://doi.org/10.3390/ijms20081939

AMA Style

Henaux L, Thibodeau J, Pilon G, Gill T, Marette A, Bazinet L. How Charge and Triple Size-Selective Membrane Separation of Peptides from Salmon Protein Hydrolysate Orientate their Biological Response on Glucose Uptake. International Journal of Molecular Sciences. 2019; 20(8):1939. https://doi.org/10.3390/ijms20081939

Chicago/Turabian Style

Henaux, Loïc, Jacinthe Thibodeau, Geneviève Pilon, Tom Gill, André Marette, and Laurent Bazinet. 2019. "How Charge and Triple Size-Selective Membrane Separation of Peptides from Salmon Protein Hydrolysate Orientate their Biological Response on Glucose Uptake" International Journal of Molecular Sciences 20, no. 8: 1939. https://doi.org/10.3390/ijms20081939

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