MoMCP1, a Cytochrome P450 Gene, Is Required for Alleviating Manganese Toxin Revealed by Transcriptomics Analysis in Magnaporthe oryzae
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China
College of Science, Yunnan Agricultural University, Kunming 650201, China
Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives, Kunming 650223, China
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(7), 1590; https://doi.org/10.3390/ijms20071590
Received: 24 February 2019 / Revised: 20 March 2019 / Accepted: 25 March 2019 / Published: 29 March 2019
(This article belongs to the Section Molecular Microbiology)
Manganese, as an essential trace element, participates in many physiological reactions by regulating Mn associated enzymes. Magnaporthe oryzae is a serious pathogen and causes destructive losses for rice production. We identified a cytochrome P450 gene, MoMCP1, involving the alleviation of manganese toxin and pathogenicity. To identify the underlying mechanisms, transcriptomics were performed. The results indicated that many pathogenicity related genes were regulated, especially hydrophobin related genes in ∆Momcp1. Furthermore, the Mn2+ toxicity decreased the expressions of genes involved in the oxidative phosphorylation and energy production, and increased the reactive oxygen species (ROS) levels, which might impair the functions of mitochondrion and vacuole, compromising the pathogenicity and development in ∆Momcp1. Additionally, our results provided further information about Mn associated the gene network for Mn metabolism in cells.