Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study
Abstract
:1. Introduction
2. Results
2.1. Body Weight and the Nociception Value
2.2. Hierarchical Cluster
2.3. GO and KEGG Pathway Enrichment
2.4. PPI Analysis
2.5. PRM Validation of TMT-Based Results
3. Discussion
4. Materials and Methods
4.1. MTrPs Modeling
4.2. Static Stretching and Dry Needling Treatment
4.3. Proteomics Analysis
4.3.1. Tandem Mass Tag (TMT) Sample Preparation
4.3.2. LC-MS/MS Analysis
4.3.3. Bioinformatics Analysis
4.4. Parallel Reaction Monitoring (PRM) Validation
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Terms | Count | P value | FDR (%) | richFactor | Protein Names (Gene Names) |
GO (gene ontology) | |||||
(BP) Single-organism carbohydrate catabolic process | 8 | 0.0006 | 0.2982 | 0.1633 | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (Phkg1); Phosphorylase b kinase regulatory subunit (Phka1); Phosphorylase b kinase regulatory subunit (Phkb); Glycogen phosphorylase, muscle form (Pygm); L-lactate dehydrogenase B chain (Ldhb); Actinin alpha 3 (Actn3); Alpha-1,4 glucan phosphorylase (Pygm); Gamma-enolase (Eno2) |
(MF) Integrin binding | 4 | 0.0125 | 0.4795 | 0.1739 | Actinin alpha 3 (Actn3); Fibrillin 1 (Fbn1); Actn1 protein (Actn1); Junctional adhesion molecule C (Jam3) |
(CC) Supramolecular fiber | 20 | 0.0040 | 0.4795 | 0.0755 | Type I keratin KA11 (Ka11); Actinin alpha 3 (Actn3); Troponin T, fast skeletal muscle (Tnnt3); Myosin-4 (Myh2); Fibrillin 1 (Fbn1); Small muscular protein (Smpx); Actn1 protein (Actn1); Tubulin alpha-1B chain (Tuba1b); Myosin, heavy chain 15 (Myh15); Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (Atp2a1); Type 2X myosin heavy chain (Myhc); Myosin-4 (Myh1); Myosin-6 (Myh6); Cysteine and glycine-rich protein 3 (Csrp3); Uncharacterized protein; Leiomodin-2 (Lmod2); Myosin binding protein C, fast-type (Mybpc2); Microfibril-associated protein 4 (Mfap4); Myozenin 2 (Myoz2); Ryanodine receptor 3 (Ryr3) |
Terms | Count | P value | FDR (%) | richFactor | Protein Names (Gene Names) |
KEGG (kyoto encyclopedia of genes and genomes) pathways | |||||
Tight junction | 6 | 0.0189 | 0.7500 | 0.1132 | Myosin-4 (Myh2); Tubulin alpha-1B chain (Tuba1b); Myosin-4 (Myh1); Junctional adhesion molecule C (Jam3); Actn1 protein (Actn1); Myosin, heavy chain 15 (Myh15) |
Glucagon signaling pathway | 6 | 0.0042 | 0.4642 | 0.1538 | L-lactate dehydrogenase B chain (Ldhb); Protein arginine methyltransferase 1-like (LOC100361025); Glycogen phosphorylase, muscle form (Pygm); Phosphorylase b kinase regulatory subunit (Phka1); Alpha-1,4 glucan phosphorylase (Pygm); Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (Phkg1) |
Sulfur metabolism | 2 | 0.0220 | 0.7501 | 0.3333 | Uncharacterized protein; Sulfite oxidase (Suox) |
Cardiac muscle contraction | 5 | 0.0464 | 0.7501 | 0.1020 | Cytochrome c oxidase subunit 1 (COI); Ubiquinol-cytochrome c reductase, complex III subunit X (Uqcr10); Myosin-6 (Myh6); Sodium/potassium-transporting ATPase subunit beta-2 (Atp1b2); Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (Atp2a1) |
Carbohydratedigestion and absorption | 2 | 0.0488 | 0.7501 | 0.2222 | Sodium/potassium-transporting ATPase subunit beta-2 (Atp1b2); Solute carrier family 37 (Glucose-6-phosphate transporter), member 4 (Slc37a4) |
Butanoate metabolism | 2 | 0.0488 | 0.7501 | 0.2222 | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (Oxct1); L-2-hydroxyglutarate dehydrogenase (L2hgdh) |
Degree | Uniprot ID | Protein Name | Gene Name |
---|---|---|---|
Actinin alpha 3 (4-connected) | D3ZA38 | Myosin binding protein C, fast-type | Mybpc2 |
P00564 | Creatine kinase M-type | Ckm | |
Q9QZV8 | Type 2X myosin heavy chain | Myhc | |
A0A0G2KAY2 | Troponin T, fast skeletal muscle | Tnnt3 | |
Type 2X myosin heavy chain (4-connected) | A0A0G2KAY2 | Troponin T, fast skeletal muscle | Tnnt3 |
Q8R4I6 | Actinin alpha 3 | Actn3 | |
Q64578 | ATPase 1 | Atp2a1 | |
A0A0G2K484 | Myosin-4 | Myh1 |
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Li, L.; Huang, Q.; Barbero, M.; Liu, L.; Nguyen, T.; Xu, A.; Ji, L. Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study. Int. J. Mol. Sci. 2019, 20, 564. https://doi.org/10.3390/ijms20030564
Li L, Huang Q, Barbero M, Liu L, Nguyen T, Xu A, Ji L. Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study. International Journal of Molecular Sciences. 2019; 20(3):564. https://doi.org/10.3390/ijms20030564
Chicago/Turabian StyleLi, Lihui, Qiangmin Huang, Marco Barbero, Lin Liu, Thitham Nguyen, Anle Xu, and Lijuan Ji. 2019. "Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study" International Journal of Molecular Sciences 20, no. 3: 564. https://doi.org/10.3390/ijms20030564