Deducting MicroRNA-Mediated Changes Common in Bronchial Epithelial Cells of Asthma and Chronic Obstructive Pulmonary Disease—A Next-Generation Sequencing-Guided Bioinformatic Approach

Tsai, Ming-Ju; Tsai, Yu-Chen; Chang, Wei-An; Lin, Yi-Shiuan; Tsai, Pei-Hsun; Sheu, Chau-Chyun; Kuo, Po-Lin; Hsu, Ya-Ling

doi:10.3390/ijms20030553

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Deducting MicroRNA-Mediated Changes Common in Bronchial Epithelial Cells of Asthma and Chronic Obstructive Pulmonary Disease—A Next-Generation Sequencing-Guided Bioinformatic Approach

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Ya-Ling Hsu

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Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 807 Kaohsiung, Taiwan

Department of Internal Medicine, School of Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan

Department of Respiratory Therapy, School of Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan

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Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan

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Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(3), 553; https://doi.org/10.3390/ijms20030553

Received: 8 December 2018 / Revised: 15 January 2019 / Accepted: 26 January 2019 / Published: 28 January 2019

(This article belongs to the Special Issue New Regulators and Modulators of MicroRNA)

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Abstract

Asthma and chronic obstructive pulmonary disease (COPD) are chronic airway inflammatory diseases that share some common features, although these diseases are somewhat different in etiologies, clinical features, and treatment policies. The aim of this study is to investigate the common microRNA-mediated changes in bronchial epithelial cells of asthma and COPD. The microRNA profiles in primary bronchial epithelial cells from asthma (AHBE) and COPD (CHBE) patients and healthy subjects (NHBE) were analyzed with next-generation sequencing (NGS) and the significant microRNA changes common in AHBE and CHBE were extracted. The upregulation of hsa-miR-10a-5p and hsa-miR-146a-5p in both AHBE and CHBE was confirmed with quantitative polymerase chain reaction (qPCR). Using bioinformatic methods, we further identified putative targets of these microRNAs, which were downregulated in both AHBE and CHBE: miR-10a-5p might suppress BCL2, FGFR3, FOXO3, PDE4A, PDE4C, and PDE7A; miR-146a-5p might suppress BCL2, INSR, PDE4D, PDE7A, PDE7B, and PDE11A. We further validated significantly decreased expression levels of FOXO3 and PDE7A in AHBE and CHBE than in NHBE with qPCR. Increased serum miR-146a-5p level was also noted in patients with asthma and COPD as compared with normal control subjects. In summary, our study revealed possible mechanisms mediated by miR-10a-5p and miR-146a-5p in the pathogenesis of both asthma and COPD. The findings might provide a scientific basis for developing novel diagnostic and therapeutic strategies. View Full-Text

Keywords: asthma; COPD; epithelium; bronchial epithelial cells; next-generation sequencing; bioinformatics

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MDPI and ACS Style

Tsai, M.-J.; Tsai, Y.-C.; Chang, W.-A.; Lin, Y.-S.; Tsai, P.-H.; Sheu, C.-C.; Kuo, P.-L.; Hsu, Y.-L. Deducting MicroRNA-Mediated Changes Common in Bronchial Epithelial Cells of Asthma and Chronic Obstructive Pulmonary Disease—A Next-Generation Sequencing-Guided Bioinformatic Approach. Int. J. Mol. Sci. 2019, 20, 553. https://doi.org/10.3390/ijms20030553

AMA Style

Tsai M-J, Tsai Y-C, Chang W-A, Lin Y-S, Tsai P-H, Sheu C-C, Kuo P-L, Hsu Y-L. Deducting MicroRNA-Mediated Changes Common in Bronchial Epithelial Cells of Asthma and Chronic Obstructive Pulmonary Disease—A Next-Generation Sequencing-Guided Bioinformatic Approach. International Journal of Molecular Sciences. 2019; 20(3):553. https://doi.org/10.3390/ijms20030553

Chicago/Turabian Style

Tsai, Ming-Ju, Yu-Chen Tsai, Wei-An Chang, Yi-Shiuan Lin, Pei-Hsun Tsai, Chau-Chyun Sheu, Po-Lin Kuo, and Ya-Ling Hsu. 2019. "Deducting MicroRNA-Mediated Changes Common in Bronchial Epithelial Cells of Asthma and Chronic Obstructive Pulmonary Disease—A Next-Generation Sequencing-Guided Bioinformatic Approach" International Journal of Molecular Sciences 20, no. 3: 553. https://doi.org/10.3390/ijms20030553

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Deducting MicroRNA-Mediated Changes Common in Bronchial Epithelial Cells of Asthma and Chronic Obstructive Pulmonary Disease—A Next-Generation Sequencing-Guided Bioinformatic Approach

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