A Novel Hepatic Anti-Fibrotic Strategy Utilizing the Secretome Released from Etanercept-Synthesizing Adipose-Derived Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Attainment of Etanercept-Secretome from Etanercept-Synthesizing ASCs
2.2. Effects of Etanercept-Secretome on the Protein Expression in HSCs in Vitro
2.3. Effects of the Etanercept-Secretome in an In Vivo Liver Fibrosis Model
2.4. Histological Alternation of Liver Specimens after Injection of Etanercept-Secretome
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Generation of Etanercept-Synthesizing ASCs
4.3. Attainment of Secretome
4.4. Cell Proliferation Assay
4.5. Design of Animal Study
4.6. Western Blot Analysis
4.7. Serology Test and ELISA
4.8. Immunohistochemistry and Masson’s Trichrome Staining
4.9. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
TNF-α | Tumor necrosis factor-α |
ASCs | ASCs adipose-derived stem cells |
HSCs | Hepatic stellate cells |
BDL | Bile duct ligation |
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Han, J.H.; Kim, O.-H.; Lee, S.C.; Kim, K.-H.; Park, J.H.; Lee, J.I.; Lee, K.H.; Hong, H.-E.; Seo, H.; Choi, H.J.; et al. A Novel Hepatic Anti-Fibrotic Strategy Utilizing the Secretome Released from Etanercept-Synthesizing Adipose-Derived Stem Cells. Int. J. Mol. Sci. 2019, 20, 6302. https://doi.org/10.3390/ijms20246302
Han JH, Kim O-H, Lee SC, Kim K-H, Park JH, Lee JI, Lee KH, Hong H-E, Seo H, Choi HJ, et al. A Novel Hepatic Anti-Fibrotic Strategy Utilizing the Secretome Released from Etanercept-Synthesizing Adipose-Derived Stem Cells. International Journal of Molecular Sciences. 2019; 20(24):6302. https://doi.org/10.3390/ijms20246302
Chicago/Turabian StyleHan, Jae Hyun, Ok-Hee Kim, Sang Chul Lee, Kee-Hwan Kim, Jung Hyun Park, Jae Im Lee, Kyung Hee Lee, Ha-Eun Hong, Haeyeon Seo, Ho Joong Choi, and et al. 2019. "A Novel Hepatic Anti-Fibrotic Strategy Utilizing the Secretome Released from Etanercept-Synthesizing Adipose-Derived Stem Cells" International Journal of Molecular Sciences 20, no. 24: 6302. https://doi.org/10.3390/ijms20246302