The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)
Abstract
:1. Introduction
2. Results
2.1. Morphology of the Mutant NWMS1 and Shengnong 1
2.2. Anther and Microspore Structures of Mutant NWMS1 and Shengnong 1
2.3. PCD Detection in Anthers
2.4. Overview of the Transcriptome Data
2.5. DEGs among the Six Samples
2.6. DEG Co-Expression Clusters
2.7. Valid Gene Co-Expression Network Analysis with WGCNA
2.8. Key Genes Involved in Abnormal Anther Development of NWMS1 Screened via WGCNA
2.9. The Expression Profiles of Key Genes Involved in Anther Development
3. Discussion
3.1. NWMS1 Belongs to Binucleate Microspore Abortion
3.2. Global Gene Transcription Changes in NWMS1 and Shengnong 1
3.3. Complex Network Regulating Anther Development of NWMS1
3.4. Starch and Sucrose Metabolism is a Major Factor Causing Male Sterility in NWMS1
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Morphology Observation
4.3. Micrstructure and Ultrastructure Observation
4.4. TUNEL Assay
4.5. Sample Preparation and RNA Extraction for RNA-seq
4.6. Transcriptome Sequencing and Data Analysis
4.7. Weighted Gene Co-Expression Network Analysis (WGCNA)
4.8. qRT-PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Traits | Shengnong 1 | NWMS1 |
---|---|---|
Plant height (cm) | 70.59 ± 1.54 | 68.19 ± 3.45 * |
Spike length (cm) | 10.6 ± 0.39 | 8.73 ± 0.51 * |
Flag leaf length (cm) | 20.02 ± 3.37 | 18.03 ± 2.03 * |
Flag leaf width (cm) | 2.3 ± 0.15 | 1.88 ± 0.19 * |
Internode length under spike (cm) | 31.83 ± 2.39 | 29.24 ± 5.21 * |
Effective tiller number | 13.4 ± 2.55 | 10.2 ± 2.53 * |
Internode number of the main stem | 5.00 ± 0.00 | 5.00 ± 0.00 |
Spikelet number on the main stem | 20.4 ± 1.35 | 19.4 ± 1.84 |
Spike type | Rectangle | Rectangle |
Awn color | White | White |
Heading stage (Month-Day) | 04-10 | 04-17 ** |
Anthesis stage (Month-Day) | 04-23 | 05-01 ** |
Maturity stage (Month-Day) | 05-22 | 05-31 ** |
Sample Pair | Class | Annotation | GO ID | Corrected p-Value |
---|---|---|---|---|
S1-NWMS1 vs. WT | biological process | response to cadmium ion | GO:0046686 | 1.9 × 10−23 |
cellular component | cytoplasmic membrane-bounded vesicle | GO:0016023 | 1.0 × 10−30 | |
molecular function | electron carrier activity | GO:0009055 | 3.9 × 10−19 | |
S2-NWMS1 vs. WT | biological process | pentose-phosphate shunt | GO:0006098 | 1.0 × 10−30 |
cellular component | cytoplasmic vesicle | GO:0016023 | 1.0 × 10−30 | |
molecular function | heme binding | GO:0020037 | 2.2 × 10−22 | |
S3-NWMS1 vs. WT | biological process | oxidation-reduction process | GO:0055114 | 4.8 × 10−25 |
cellular component | cytoplasmic membrane-bounded vesicle | GO:0016023 | 1.0 × 10−30 | |
molecular function | heme binding | GO:0020037 | 1.6 × 10−29 |
Sample Pair | Pathway | Ko ID | Corrected p-Value |
---|---|---|---|
S1-NWMS1 vs. WT | amino sugar and nucleotide sugar metabolism | ko00520 | 0.031039284 |
S2-NWMS1 vs. WT | phenylalanine metabolism | ko00360 | 0 |
phenylpropanoid biosynthesis | ko00940 | 0 | |
glutathione metabolism | ko00480 | 1.50 × 10−10 | |
S3-NWMS1 vs. WT | phenylalanine metabolism | ko00360 | 0 |
glutathione metabolism | ko00480 | 0 | |
starch and sucrose metabolism | ko00500 | 0 |
Gene ID | Modules | Clusters | S1_NWMS1 | S1_WT | S2_NWMS1 | S2_WT | S3_NWMS1 | S3_WT | Gene Name |
---|---|---|---|---|---|---|---|---|---|
TraesCS2D01G420100 | light cyan | K3 | 0.00 | 0.00 | 21.36 | 14.53 | 5.23 | 0.50 | SKP1B |
TraesCS7B01G213800 | light cyan | K3 | 0.00 | 0.02 | 3.35 | 2.62 | 0.81 | 0.02 | Os03g0802700 |
TraesCS7A01G430600 | light cyan | K3 | 0.01 | 0.00 | 21.98 | 16.60 | 5.20 | 0.09 | BIP5 |
TraesCS4B01G248600 | light cyan | K3 | 2.07 | 2.56 | 43.93 | 34.65 | 12.54 | 0.68 | BGLU6 |
TraesCS2D01G373700 | light cyan | K3 | 0.00 | 0.00 | 41.16 | 23.75 | 4.36 | 0.42 | F775_16360 |
TraesCS5A01G285900 | black | K3 | 0.00 | 0.00 | 2.95 | 5.69 | 2.04 | 0.03 | PAP27 |
TraesCS1A01G413400 | black | K3 | 0.00 | 0.00 | 9.60 | 18.89 | 5.89 | 0.49 | KCS11 |
TraesCS2B01G056100 | black | K3 | 0.00 | 0.00 | 23.47 | 43.09 | 11.88 | 0.05 | SWEET6B |
TraesCS1D01G376300 | black | K3 | 0.09 | 0.10 | 8.40 | 17.96 | 6.75 | 0.25 | ADH3 |
TraesCS7A01G536800 | black | K3 | 0.01 | 0.00 | 8.33 | 32.95 | 6.53 | 0.12 | At5g03610 |
TraesCS4D01G296000 | green | K2 | 0.41 | 0.04 | 41.70 | 16.73 | 65.78 | 5.44 | TIFY10B |
TraesCS3B01G558400 | green | K2 | 0.60 | 0.52 | 8.20 | 5.28 | 15.95 | 1.75 | CSLA11 |
TraesCS6D01G325100 | green | K2 | 0.54 | 0.89 | 44.31 | 29.72 | 94.55 | 9.71 | SDH |
TraesCS6A01G259200 | green | K2 | 0.21 | 0.02 | 18.59 | 7.12 | 31.49 | 2.80 | ATL41 |
TraesCS2A01G276000 | green | K2 | 0.10 | 0.09 | 4.03 | 1.87 | 7.64 | 0.69 | EXO70B1 |
TraesCS1D01G270100 | brown | K4 | 0.06 | 0.00 | 0.25 | 8.62 | 112.57 | 445.69 | GASA2 |
TraesCS3D01G066700 | brown | K4 | 0.00 | 0.00 | 1.20 | 10.69 | 126.30 | 507.99 | F775_31707 |
TraesCS5D01G282500 | brown | K4 | 0.00 | 0.01 | 0.09 | 1.87 | 34.21 | 146.67 | SAG12 |
TraesCS1B01G353800 | brown | K4 | 0.00 | 0.01 | 0.04 | 0.74 | 21.35 | 94.06 | STP8 |
TraesCS4B01G125500 | brown | K6 | 0.00 | 0.00 | 0.04 | 0.56 | 4.34 | 23.94 | At1g26850 |
Gene ID | S1_NWMS1 | S1_WT | S2_NWMS1 | S2_WT | S3_NWMS1 | S3_WT | Gene Name | Reported Function |
---|---|---|---|---|---|---|---|---|
novelGene_53829 | 0.00 | 0.00 | 0.22 | 0.03 | 0.01 | 0.02 | PAIR1 | 3 |
novelGene_56612 | 0.20 | 0.23 | 0.09 | 0.20 | 0.22 | 0.16 | PAIR2 | 3 |
TraesCS7A01G316600 | 1.40 | 1.26 | 0.79 | 0.56 | 0.96 | 1.11 | PSS1 | 3, 5 |
TraesCS2A01G226000 | 8.31 | 8.92 | 2.03 | 2.59 | 1.98 | 1.69 | BUB1 | 2, 3 |
TraesCS6A01G364300 | 8.81 | 9.02 | 1.34 | 1.02 | 1.60 | 1.39 | SGO1 | 2, 3 |
TraesCS7A01G309300 | 0.48 | 0.47 | 260.82 | 220.0 | 26.93 | 0.83 | CYP703A2 | 4, 5, 6 |
novelGene_128161 | 0.54 | 0.68 | 3.13 | 1.28 | 0.56 | 0.26 | MYB80 | 4, 6 |
novelGene_128276 | 0.00 | 0.07 | 0.08 | 0.00 | 0.06 | 0.09 | DEX1 | 4, 5 |
TraesCS1B01G247200 | 4.19 | 5.16 | 9.20 | 8.53 | 9.42 | 7.83 | DAD1 | 5, 6 |
TraesCS7A01G305700 | 2.57 | 2.33 | 0.36 | 0.59 | 0.19 | 0.24 | TDR | 4 |
TraesCS7A01G458700 | 0.00 | 0.00 | 0.00 | 0.03 | 0.17 | 25.50 | GAMYB | 5, 6 |
TraesCS5A01G481600 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | BAM1 | 1 |
TraesCS1A01G444500 | 3.78 | 3.90 | 0.23 | 0.11 | 0.18 | 0.12 | BAM2 | 1 |
TraesCS2D01G407700 | 28.12 | 32.21 | 183.32 | 193.7 | 192.5 | 20.81 | CSA | 6, 7 |
novelGene_21887 | 0.02 | 0.00 | 0.01 | 0.00 | 0.00 | 0.20 | TDL1B | 1, 3 |
novelGene_139463 | 2.67 | 2.34 | 3.78 | 2.67 | 2.63 | 2.35 | MSP1 | 1, 3 |
TraesCS2A01G423900 | 1.95 | 2.32 | 91.01 | 82.45 | 11.51 | 5.97 | QRT3 | 6 |
Enzyme D | Enzyme Code * | S1-Log2 (NWMS1/WT) ** | S2-Log2 (NWMS1/WT) | S3-Log2 (NWMS1/WT) |
---|---|---|---|---|
glycogen phosphorylase | 2.4.1.1 | − | −1.2 | −5.64 |
sucrose synthase | 2.4.1.13 | − | −2 | 5.07 & −2.0 |
sucrose-phosphate synthase | 2.4.1.14 | − | −2.5 | −1.24 |
trehalose 6-phosphate synthase | 2.4.1.15 | − | 1.2 & −1.5 | 4.48 & −2.86 |
1,4-alpha-glucan branching enzyme | 2.4.1.18 | − | − | 1.43 & −3.96 |
starch synthase | 2.4.1.21 | − | −1.2 | 1.24 & −1.79 |
4-alpha-glucanotransferase | 2.4.1.25 | − | −1.23 | −2.72 |
Hexokinase | 2.7.1.1 | − | 3.2 & −1.0 | 5.14 & −3.74 |
Fructokinase | 2.7.1.4 | − | 1.2 & −1.5 | 3.62 & −2.58 |
glucose-1-phosphate adenylyltransferase | 2.7.7.27 | − | −1.2 | −3.56 |
UTP-glucose-1-phosphate uridylyltransferase | 2.7.7.9 | − | − | −8.72 |
trehalose 6-phosphate phosphatase | 3.1.3.12 | 1.67 & −10.25 | 3.0 & −1.5 | 3.16 & −1.68 |
alpha-amylase | 3.2.1.1 | − | −2.78 | 9.25 & −1.30 |
beta-amylase | 3.2.1.2 | − | 1 | 2.10 & −3.89 |
alpha-glucosidase | 3.2.1.20 | − | 1.8 | 1.99 & −2.03 |
beta-glucosidase | 3.2.1.21 | 1.47 | − | 2.54 & −2.65 |
eta-fructofuranosidase | 3.2.1.26 | −1.01 | 1.1 & −2.1 | 1.67 & −3.28 |
alpha, alpha-trehalase | 3.2.1.28 | − | − | 2.46 |
glucan endo-1,3-beta-glucosidase 1/2/3 | 3.2.1.39 | −1.88 | 1.2 & −1.7 | 1.92 & −1.56 |
Endoglucanase | 3.2.1.4 | − | 2 | −6.85 |
ADP-sugar diphosphatase | 3.6.1.21 | − | − | −2.75 |
Phosphoglucomutase | 5.4.2.2 | − | − | −1.71 |
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Li, J.; Zhang, J.; Li, H.; Niu, H.; Xu, Q.; Jiao, Z.; An, J.; Jiang, Y.; Li, Q.; Niu, J. The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.). Int. J. Mol. Sci. 2019, 20, 6252. https://doi.org/10.3390/ijms20246252
Li J, Zhang J, Li H, Niu H, Xu Q, Jiao Z, An J, Jiang Y, Li Q, Niu J. The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.). International Journal of Molecular Sciences. 2019; 20(24):6252. https://doi.org/10.3390/ijms20246252
Chicago/Turabian StyleLi, Junchang, Jing Zhang, Huijuan Li, Hao Niu, Qiaoqiao Xu, Zhixin Jiao, Junhang An, Yumei Jiang, Qiaoyun Li, and Jishan Niu. 2019. "The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)" International Journal of Molecular Sciences 20, no. 24: 6252. https://doi.org/10.3390/ijms20246252