MicroRNA-137 Inhibits Cancer Progression by Targeting Del-1 in Triple-Negative Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Negative Correlation between miR-137 and Del-1 Expression in TNBC Cell Lines
2.2. MiR-137 Directly Targets the 3′-UTR of Del-1 to Inhibit Del-1 Expression
2.3. miR-137 Regulation of Endogenous Del-1 Expression
2.4. miR-137 Inhibits MDA-MB-231 Cell Proliferation, Migration, and Invasion
2.5. Del-1 Expression Regulates Proliferation, Migration, and Invasion of MDA-MB-231 Cells
2.6. Expression of miR-137 and Del-1 in TNBC Patients
3. Discussion
4. Materials and Methods
4.1. Candidate miRNA Selection
4.2. Cell Lines and Cell Culture
4.3. RNA Extraction and Quantitative RT-PCR
4.4. miRNA Transfection
4.5. Plasmid Vector Construction for Luciferase Reporter Assays and Mutagenesis
4.6. Dual-Luciferase Reporter Assay
4.7. ELISA
4.8. MTT Assay for Cell Proliferation
4.9. Wound Scratch Assay for Cell Migration
4.10. Transwell Assay for Cell Invasion
4.11. Expression of miR-137 and Del-1 in Tissues
4.12. Knockdown and Overexpression of Del-1 in MDA-MB-231 Cells
4.13. Statistical Analyses
4.14. Ethical Considerations
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
HER2 | human epidermal growth factor receptor 2 |
Del-1 | developmental endothelial locus-1 |
Edil-3 | epidermal growth factor-like repeats and discoidin domains-3 |
WT | wild type |
Mut | mutant type |
ELISA | enzyme-linked immunosorbent assay |
NC | control group |
siRNA | small-interfering RNA |
TNBC | triple negative breast cancer |
SD | standard deviation |
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No. | miRNA Name | Predicted Database |
---|---|---|
1 | miR-137 | Miranda, TargetScan, miRDB |
2 | miR-496 | Miranda, TargetScan, miRDB |
3 | miR-361-5P | TargetScan, miRDB |
4 | miR-425 | TargetScan, miRDB |
5 | miR-9500 | TargetScan, miRDB |
6 | miR-2113 | TargetScan, miRDB |
7 | miR-3685 | TargetScan, miRDB |
8 | miR-3163 | TargetScan, miRDB |
9 | miR-4672 | TargetScan, miRDB |
10 | miR-3662 | TargetScan, miRDB |
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Lee, S.J.; Jeong, J.-H.; Kang, S.H.; Kang, J.; Kim, E.A.; Lee, J.; Jung, J.H.; Park, H.Y.; Chae, Y.S. MicroRNA-137 Inhibits Cancer Progression by Targeting Del-1 in Triple-Negative Breast Cancer Cells. Int. J. Mol. Sci. 2019, 20, 6162. https://doi.org/10.3390/ijms20246162
Lee SJ, Jeong J-H, Kang SH, Kang J, Kim EA, Lee J, Jung JH, Park HY, Chae YS. MicroRNA-137 Inhibits Cancer Progression by Targeting Del-1 in Triple-Negative Breast Cancer Cells. International Journal of Molecular Sciences. 2019; 20(24):6162. https://doi.org/10.3390/ijms20246162
Chicago/Turabian StyleLee, Soo Jung, Jae-Hwan Jeong, Seung Hee Kang, Jieun Kang, Eun Ae Kim, Jeeyeon Lee, Jin Hyang Jung, Ho Yong Park, and Yee Soo Chae. 2019. "MicroRNA-137 Inhibits Cancer Progression by Targeting Del-1 in Triple-Negative Breast Cancer Cells" International Journal of Molecular Sciences 20, no. 24: 6162. https://doi.org/10.3390/ijms20246162