Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes
Abstract
:1. Introduction
2. Results
2.1. DHA-Treated Adipocytes Upregulates the Expression of Corin and ANP
2.2. The Effect of DHA on ANP Secretion is Mediated by PKC/ERK Pathway
2.3. The Expression of Corin and ANP is Increased in Beige-Like Adipocytes
2.4. ANP Derived from Beige-Like Adipocytes Enhances cGMP Levels in HEK-293 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Adipocyte Differentiation
4.2. RNA-Seq Analysis
4.3. Quantitative Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
4.4. Western Blot Analysis
4.5. Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Statistical analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Sequence |
---|---|
β-actin | (F)5′-GTGACGTTGACATCCGTAAAGA-3′ |
(R)5′-GCCGGACTCATCGTACTCC-3′ | |
UCP1 | (F)5′-AGGCTTCCAGTACCATTAGGT-3′ |
(R)5′-CTGAGTGAGGCAAAGCTGATTT-3′ | |
Tnfrsf9 | (F)5′-CGTGCAGAACTCCTGTGATAA C-3′ |
(R)5′-GTCCACCTATGCTGGAGAAGG-3′ | |
Cited1 | (F)5′-AACCTTGGAGTGAAGGATCGC-3′ |
(R)5′-GTAGGAGAGCCTATTGGAGATGT-3′ | |
Eva1a | (F)5′-GGGGAGACCGAAGGAAATGAGA-3′ |
(R)5′-CTCCAGCCCTGCACACTCTA-3′ | |
PDK4 | (F)5′-AGGGAGGTCGAGCTGTTCTC-3′ |
(R)5′-GGAGTGTTCACTAAGCGGTCA-3′ | |
Cox7a1 | (F)5′-GCTCTGGTCCGGTCTTTTAGC-3′ |
(R)5′-GTACTGGGAGGTCATTGTCGG-3′ | |
Corin | (F)5′-GCTGGTGACTGCTAACTTGCT-3′ |
(R)5′-CCCATCAGTGACCAAAGGTTC-3′ | |
ANP | (F)5′-TCGTCTTGGCCTTTTGGCT-3′ |
(R)5′-TCCAGGTGGTCTAGCAGGTTCT-3′ | |
NPR1 | (F)5′-GGGATACAGTCAACACAGCCTCAA-3′ |
(R)5′-CGAAGCTCCAGCTCGAAA C-3′ |
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Bae, I.-S.; Kim, S.H. Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes. Int. J. Mol. Sci. 2019, 20, 6128. https://doi.org/10.3390/ijms20246128
Bae I-S, Kim SH. Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes. International Journal of Molecular Sciences. 2019; 20(24):6128. https://doi.org/10.3390/ijms20246128
Chicago/Turabian StyleBae, In-Seon, and Sang Hoon Kim. 2019. "Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes" International Journal of Molecular Sciences 20, no. 24: 6128. https://doi.org/10.3390/ijms20246128