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Article

Renal Tubular Glucagon-Like Peptide-1 Receptor Expression Is Increased in Early Sepsis but Reduced in Chronic Kidney Disease and Sepsis-Induced Kidney Injury

1
Department of Internal Medicine, Graduate School of Medicine, Chungbuk National University, Chungbuk 28644, Korea
2
Department of Internal Medicine, Chungbuk National University Hospital, College of Medicine, Chungbuk National University, Chungbuk 28644, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6024; https://doi.org/10.3390/ijms20236024
Received: 10 November 2019 / Revised: 26 November 2019 / Accepted: 27 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Kidney Injury: From Molecular Basis to Therapies 2.0)
Acute kidney injury (AKI) is common in patients with sepsis and causes renal ischemia. Glucagon-like peptide-1 (GLP-1) protects the vascular system and the kidney, and GLP-1 receptor (GLP-1R) is expressed in the kidney. Renal GLP-1R activity is decreased in chronic kidney disease (CKD), but is increased by the inflammatory response; however, the effect of AKI on GLP-1R expression is unknown. We investigated the role of GLP-1 by assessing GLP-1R expression in the renal cortex in animals with AKI-related sepsis, CKD, and CKD-with-sepsis. We generated a model of CKD by 5/6 nephrectomy, and sepsis induced by cecal perforation, in male Sprague–Dawley rats. We compared renal GLP-1R expression at 3, 6, 12, 24, and 72 h after cecal perforation, and in CKD and CKD-with-sepsis. We performed blood and urine tests, western blotting (WB), and immunohistochemistry (IHC) to assay GLP-1R expression in renal tubules. The CKD-with-sepsis group showed the lowest kidney function, urine volume, and serum glucose and albumin levels. GLP-1R expression in renal tubules was decreased at 3 h, increased at 24 h, and decreased at 72 h after sepsis induction. GLP-1R expression was decreased at 8 weeks after CKD and was lowest in the CKD-with-sepsis group. The WB results were verified against those obtained by IHC. GLP-1R expression in renal tubules is increased in early sepsis, which may explain the protective effect of endogenous GLP-1 against sepsis-related inflammation. View Full-Text
Keywords: GLP-1; GLP-1 receptor; sepsis; acute kidney injury; chronic kidney disease GLP-1; GLP-1 receptor; sepsis; acute kidney injury; chronic kidney disease
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MDPI and ACS Style

Choi, J.H.; Kim, S.J.; Kwon, S.K.; Kim, H.-Y.; Jeon, H. Renal Tubular Glucagon-Like Peptide-1 Receptor Expression Is Increased in Early Sepsis but Reduced in Chronic Kidney Disease and Sepsis-Induced Kidney Injury. Int. J. Mol. Sci. 2019, 20, 6024. https://doi.org/10.3390/ijms20236024

AMA Style

Choi JH, Kim SJ, Kwon SK, Kim H-Y, Jeon H. Renal Tubular Glucagon-Like Peptide-1 Receptor Expression Is Increased in Early Sepsis but Reduced in Chronic Kidney Disease and Sepsis-Induced Kidney Injury. International Journal of Molecular Sciences. 2019; 20(23):6024. https://doi.org/10.3390/ijms20236024

Chicago/Turabian Style

Choi, Jae H., Seung J. Kim, Soon K. Kwon, Hye-Young Kim, and Hyunjung Jeon. 2019. "Renal Tubular Glucagon-Like Peptide-1 Receptor Expression Is Increased in Early Sepsis but Reduced in Chronic Kidney Disease and Sepsis-Induced Kidney Injury" International Journal of Molecular Sciences 20, no. 23: 6024. https://doi.org/10.3390/ijms20236024

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