Inhibition of the Low Molecular Weight Protein Tyrosine Phosphatase (LMPTP) as a Potential Therapeutic Strategy for Hepatic Progenitor Cells Lipotoxicity—Short Communication
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Isolation and Culture
2.2. In Vitro Study
2.3. RNA Isolation and Gene Expression Profiling Analysis
2.4. Statistical Analysis
3. Results
3.1. HPC Genotype and Morphology
3.2. Expression of Apoptosis-Related Genes
3.3. LMPTP Inhibition Alters Expression of Genes Involved in Autophagy, Mitochondria Dynamics, and Unfolded Protein Response (UPR) in IR-Induced HPCs
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequence 5′→3′ | Amplicon Length (bp) | Accession No. |
---|---|---|---|
HNF4A | F: CAGGAGATGCTGCTGGGAG R: ATTGTGGTGATGGCTCCTGG | 257 | XM_014735168.2 |
PECAM1 | F: ACACGGAAGTGGAAGTGACC R: CCATCAAGGGAGCCTTCCG | 142 | NM_001101655.2 |
AFP | F: CAGCCACTTGTTGCCAACTC R: CTGGCCAACACCAGGGTTTA | 125 | NM_001081952.1 |
ALAT | F: GGGAAGGCACCTACCACTTC R: ACTTGGCATGGAACTGGCTT | 93 | XM_005613421.2 |
CD90 | F: CTCCCACCCCTGGTGAAAACT R: CGGTGGTATTCTCATGGCGG | 257 | XM_001503225.4 |
SOX9 | F: GAACGCCTTCATGGTGTGGG R: TTCTTCACCGACTTCCTCCG | 225 | XM_014736619.1 |
CD105 | F: GACTGCCTTTGTGCAGTTGG R: ATGCTTTCGGGGTCCTTCAG | 198 | XM_003364144.4 |
EPCAM | F: TTGCCGTCATTGTGGTTGTG R: TCAGCCTTCTCGTACTTCGC | 97 | XM_001917795.4 |
P53 | F: TACTCCCCTGCCCTCAACAA R: AGGAATCAGGGCCTTGAGGA | 252 | U37120.1 |
BCl-2 | F: TTCTTTGAGTTCGGTGGGGT R: GGGCCGTACAGTTCCACAA | 164 | XM_014843802.1 |
BAX | F: CGAGTGGCAGCTGAGATGTT R: AAGGAAGTCCAGTGTCCAGC | 153 | XM_023650077.1 |
BECN1 | F: GATGCGTTATGCCCAGATGC R: ATCCAGCGAACACTCTTGGG | 147 | XM_014729146.1 |
SQSTM1 | F: CATCGGAGGATCCCAGTGTG R: CCGGTTTGTTAGGGTCGGAA | 207 | XM_005599173.3 |
LC3 | F: TTCTGAGACACAGTCGGAGC R: CTTTGTTCGAAGGTGTGGCG | 128 | XM_001493613.6 |
HSC70 | F: GATTAACAAGAGGGCTGTCCGTC R: GCCTGGGTGCTAGAAGAGAGA | 122 | XM_023628864.1 |
LAMP2 | F: GCACCCCTGGGAAGTTCTTA R: ATCCAGCGAACACTCTTGGG | 147 | XM_014831347.1 |
MFN1 | F: AAGTGGCATTTTTCGGCAGG R: TCCATATGAAGGGCATGGGC | 217 | XM_014838357.1 |
FIS1 | F: GGTGCGAAGCAAGTACAACG R: GTTGCCCACAGCCAGATAGA | 118 | XM_014854003.1 |
PINK1 | F: GCACAATGAGCCAGGAGCTA R: GGGGTATTCACGCGAAGGTA | 298 | XM_014737247.1 |
PRKN | F: TCCCAGTGGAGGTCGATTCT R: CCCTCCAGGTGTGTTCGTTT | 218 | XM_014858374.1 |
PGC1A | F: TCTACCTAGGATGCATGG R: GTGCAAGTAGAAACACTGC | 93 | XM_005608845.2 |
PERK | F: GTGACTGCAATGGACCAGGA R: TCACGTGCTCACGAGGATATT | 283 | XM_023618757.1 |
CHOP | F: AGCCAAAATCAGAGCCGGAA R: GGGGTCAAGAGTGGTGAAGG | 272 | XM_001488999.4 |
ATF6 | F: CAGGGTGCACTAGAACAGGG R: AATGTGTCTCCCCTTCTGCG | 164 | XM_023640315.1 |
HSPA5 | F: CTGTAGCGTATGGTGCTGCT R: CATGACACCTCCCACGGTTT | 122 | XM_023628864.1 |
IRE1A | F: GAATCAGACGAGCACCCGAA R: TTTCTTGCAGAGGCCGAAGT | 300 | XM_023652216.1 |
XBP1 | F: TTACGCGAGAAAACTCATGGCC R: GGGTCCAAGTTGAACAGAATGC | 281 (unspliced) 255 (spliced) | XM_014742035.2 |
GAPDH | F: GATGCCCCAATGTTTGTGA R: AAGCAGGGATGATGTTCTGG | 250 | NM 001163856.1 |
ACTB | F: GATGATGATATCGCCGCGCTC R: CGCAGCTCGTTGTAGAAGGT | 281 | NM_001081838.1 |
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Alicka, M.; Kornicka-Garbowska, K.; Roecken, M.; Marycz, K. Inhibition of the Low Molecular Weight Protein Tyrosine Phosphatase (LMPTP) as a Potential Therapeutic Strategy for Hepatic Progenitor Cells Lipotoxicity—Short Communication. Int. J. Mol. Sci. 2019, 20, 5873. https://doi.org/10.3390/ijms20235873
Alicka M, Kornicka-Garbowska K, Roecken M, Marycz K. Inhibition of the Low Molecular Weight Protein Tyrosine Phosphatase (LMPTP) as a Potential Therapeutic Strategy for Hepatic Progenitor Cells Lipotoxicity—Short Communication. International Journal of Molecular Sciences. 2019; 20(23):5873. https://doi.org/10.3390/ijms20235873
Chicago/Turabian StyleAlicka, Michalina, Katarzyna Kornicka-Garbowska, Michael Roecken, and Krzysztof Marycz. 2019. "Inhibition of the Low Molecular Weight Protein Tyrosine Phosphatase (LMPTP) as a Potential Therapeutic Strategy for Hepatic Progenitor Cells Lipotoxicity—Short Communication" International Journal of Molecular Sciences 20, no. 23: 5873. https://doi.org/10.3390/ijms20235873