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Communication

Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression

Section on Cellular Neurobiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
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Int. J. Mol. Sci. 2019, 20(22), 5725; https://doi.org/10.3390/ijms20225725
Received: 11 October 2019 / Revised: 6 November 2019 / Accepted: 14 November 2019 / Published: 15 November 2019
(This article belongs to the Section Molecular Oncology)
Pancreatic cancer is one of the leading causes of cancer-related mortality worldwide. The molecular basis for the pathogenesis of this disease remains elusive. In this study, we have investigated the role of wild-type Carboxypeptidase E (CPE-WT) and a 40 kDa N-terminal truncated isoform, CPE-ΔN in promoting proliferation and invasion of Panc-1 cells, a pancreatic cancer cell line. Both CPE-WT and CPE-ΔN were expressed in Panc-1 and BXPC-3 pancreatic cancer cells. Immunocytochemical studies revealed that in CPE transfected Panc-1 cells, CPE-ΔN was found primarily in the nucleus, whereas CPE-WT was present exclusively in the cytoplasm as puncta, characteristic of secretory vesicles. Endogenous CPE-WT was secreted into the media. Overexpression of CPE-ΔN in Panc-1 cells resulted in enhancement of proliferation and invasion of these cells, as determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell proliferation assay and Matrigel invasion assay, respectively. In contrast, the expression of CPE-WT protein at comparable levels to CPE-ΔN in Panc-1 cells resulted in promotion of proliferation but not invasion. Importantly, there was an upregulation of the expression of CXCR2 mRNA and protein in Panc-1 cells overexpressing CPE-ΔN, and these cells exhibited significant increase in proliferation in a CXCR2-dependent manner. Thus, CPE-ΔN may play an important role in promoting pancreatic cancer growth and malignancy through upregulating the expression of the metastasis-related gene, CXCR2. View Full-Text
Keywords: Carboxypeptidase E; pancreatic cancer; cell proliferation; cell invasion Carboxypeptidase E; pancreatic cancer; cell proliferation; cell invasion
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MDPI and ACS Style

Hareendran, S.; Yang, X.; Lou, H.; Xiao, L.; Loh, Y.P. Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression. Int. J. Mol. Sci. 2019, 20, 5725. https://doi.org/10.3390/ijms20225725

AMA Style

Hareendran S, Yang X, Lou H, Xiao L, Loh YP. Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression. International Journal of Molecular Sciences. 2019; 20(22):5725. https://doi.org/10.3390/ijms20225725

Chicago/Turabian Style

Hareendran, Sangeetha, Xuyu Yang, Hong Lou, Lan Xiao, and Y. Peng Loh. 2019. "Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression" International Journal of Molecular Sciences 20, no. 22: 5725. https://doi.org/10.3390/ijms20225725

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