Next Article in Journal
rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations
Previous Article in Journal
Current Approaches in NSCLC Targeting K-RAS and EGFR
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
IJMS
Volume 20
Issue 22
10.3390/ijms20225703
ijms-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Provitamin A Biofortification of Durum Wheat through a TILLING Approach

by
Francesco Sestili
1,
Maria Dolores Garcia-Molina
1,
Gianluca Gambacorta
1,
Romina Beleggia
2,
Ermelinda Botticella
1,
Pasquale De Vita
2,
Daniel Valentin Savatin
1,
Stefania Masci
1 and
Domenico Lafiandra
1,*
1
Department of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, Italy
2
Council for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5703; https://doi.org/10.3390/ijms20225703
Received: 22 October 2019 / Revised: 8 November 2019 / Accepted: 12 November 2019 / Published: 14 November 2019
(This article belongs to the Section Molecular Plant Sciences)
View Full-Text Download PDF
Browse Figures

Abstract

Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a “block strategy” perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat. View Full-Text
Keywords: durum wheat; β-carotene; TILLING; biofortification; vitamin A deficiency durum wheat; β-carotene; TILLING; biofortification; vitamin A deficiency
►▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Sestili, F.; Garcia-Molina, M.D.; Gambacorta, G.; Beleggia, R.; Botticella, E.; De Vita, P.; Savatin, D.V.; Masci, S.; Lafiandra, D. Provitamin A Biofortification of Durum Wheat through a TILLING Approach. Int. J. Mol. Sci. 2019, 20, 5703. https://doi.org/10.3390/ijms20225703

AMA Style

Sestili F, Garcia-Molina MD, Gambacorta G, Beleggia R, Botticella E, De Vita P, Savatin DV, Masci S, Lafiandra D. Provitamin A Biofortification of Durum Wheat through a TILLING Approach. International Journal of Molecular Sciences. 2019; 20(22):5703. https://doi.org/10.3390/ijms20225703

Chicago/Turabian Style

Sestili, Francesco, Maria D. Garcia-Molina, Gianluca Gambacorta, Romina Beleggia, Ermelinda Botticella, Pasquale De Vita, Daniel V. Savatin, Stefania Masci, and Domenico Lafiandra. 2019. "Provitamin A Biofortification of Durum Wheat through a TILLING Approach" International Journal of Molecular Sciences 20, no. 22: 5703. https://doi.org/10.3390/ijms20225703

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop