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Open AccessArticle

Expression Analysis of mRNA Decay of Maternal Genes during Bombyx mori Maternal-to-Zygotic Transition

by Meirong Zhang 1,2,†, Pingzhen Xu 1,2,†, Huilin Pang 1, Tao Chen 1,2,*,‡ and Guozheng Zhang 1,2,*,‡
1
School of Biotechnology, Jiangsu University of Science and Technology, Sibaidu Rd, Zhenjiang 212018, China
2
Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Sibaidu Rd, Zhenjiang 212018, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(22), 5651; https://doi.org/10.3390/ijms20225651
Received: 31 August 2019 / Revised: 6 November 2019 / Accepted: 6 November 2019 / Published: 12 November 2019
(This article belongs to the Special Issue Regulation of Gene Expression During Embryonic Development)
Maternal genes play an important role in the early embryonic development of the silkworm. Early embryonic development without new transcription depends on maternal components stored in the egg during oocyte maturation. The maternal-to-zygotic transition (MZT) is a tightly regulated process that includes maternal mRNAs elimination and zygotic transcription initiation. This process has been extensively studied within model species. Each model organism has a unique pattern of maternal transcriptional clearance classes in MZT. In this study, we identified 66 maternal genes through bioinformatics analysis and expression analysis in the eggs of silkworm virgin moths (Bombyx mori). All 66 maternal genes were expressed in vitellogenesis in day eight female pupae. During MZT, the degradation of maternal gene mRNAs could be divided into three clusters. We found that eight maternal genes of cluster 1 remained stable from 0 to 3.0 h, 17 maternal genes of cluster 2 were significantly decayed from 0.5 to 1.0 h and 41 maternal genes of cluster 3 were significantly decayed after 1.5 h. Therefore, the initial time-point of degradation of cluster 2 was earlier than that of cluster 3. The maternal gene mRNAs decay of clusters 2 and 3 is first initiated by maternal degradation activity. Our study expands upon the identification of silkworm maternal genes and provides a perspective for further research of the embryo development in Bombyx mori. View Full-Text
Keywords: Bombyx mori; maternal gene; decay; embryonic development; maternal-to zygotic-transition Bombyx mori; maternal gene; decay; embryonic development; maternal-to zygotic-transition
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Zhang, M.; Xu, P.; Pang, H.; Chen, T.; Zhang, G. Expression Analysis of mRNA Decay of Maternal Genes during Bombyx mori Maternal-to-Zygotic Transition. Int. J. Mol. Sci. 2019, 20, 5651.

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