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Open AccessArticle

Comparative Proteomic and Morpho-Physiological Analyses of Maize Wild-Type Vp16 and Mutant vp16 Germinating Seed Responses to PEG-Induced Drought Stress

by Songtao Liu 1,2, Tinashe Zenda 1,2, Anyi Dong 1,2, Yatong Yang 1,2, Xinyue Liu 1,2, Yafei Wang 1,2, Jiao Li 1,2, Yongsheng Tao 1,2,* and Huijun Duan 1,2,*
1
Department of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding 071001, China
2
North China Key Laboratory for Crop Germplasm Resources of the Education Ministry, Hebei Agricultural University, Baoding 071001, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5586; https://doi.org/10.3390/ijms20225586
Received: 11 October 2019 / Revised: 31 October 2019 / Accepted: 7 November 2019 / Published: 8 November 2019
(This article belongs to the Special Issue Molecular Mechanisms of Maize Response to Environmental Challenges)
Drought stress is a major abiotic factor compromising plant cell physiological and molecular events, consequently limiting crop growth and productivity. Maize (Zea mays L.) is among the most drought-susceptible food crops. Therefore, understanding the mechanisms underlying drought-stress responses remains critical for crop improvement. To decipher the molecular mechanisms underpinning maize drought tolerance, here, we used a comparative morpho-physiological and proteomics analysis approach to monitor the changes in germinating seeds of two incongruent (drought-sensitive wild-type Vp16 and drought-tolerant mutant vp16) lines exposed to polyethylene-glycol-induced drought stress for seven days. Our physiological analysis showed that the tolerant line mutant vp16 exhibited better osmotic stress endurance owing to its improved reactive oxygen species scavenging competency and robust osmotic adjustment as a result of greater cell water retention and enhanced cell membrane stability. Proteomics analysis identified a total of 1200 proteins to be differentially accumulated under drought stress. These identified proteins were mainly involved in carbohydrate and energy metabolism, histone H2A-mediated epigenetic regulation, protein synthesis, signal transduction, redox homeostasis and stress-response processes; with carbon metabolism, pentose phosphate and glutathione metabolism pathways being prominent under stress conditions. Interestingly, significant congruence (R2 = 81.5%) between protein and transcript levels was observed by qRT-PCR validation experiments. Finally, we propose a hypothetical model for maize germinating-seed drought tolerance based on our key findings identified herein. Overall, our study offers insights into the overall mechanisms underpinning drought-stress tolerance and provides essential leads into further functional validation of the identified drought-responsive proteins in maize. View Full-Text
Keywords: proteome profiling; iTRAQ; polyethylene glycol (PEG)-induced drought; energy metabolism; Zea mays L. proteome profiling; iTRAQ; polyethylene glycol (PEG)-induced drought; energy metabolism; Zea mays L.
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MDPI and ACS Style

Liu, S.; Zenda, T.; Dong, A.; Yang, Y.; Liu, X.; Wang, Y.; Li, J.; Tao, Y.; Duan, H. Comparative Proteomic and Morpho-Physiological Analyses of Maize Wild-Type Vp16 and Mutant vp16 Germinating Seed Responses to PEG-Induced Drought Stress. Int. J. Mol. Sci. 2019, 20, 5586.

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