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Open AccessArticle

Blood Compatibility—An Important but Often Forgotten Aspect of the Characterization of Antimicrobial Peptides for Clinical Application

1
Department for Biomedical Research, Danube University Krems, 3500 Krems, Austria
2
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, 1090 Vienna, Austria
3
Institute of Molecular Biosciences, University Graz, 8010 Graz, Austria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(21), 5426; https://doi.org/10.3390/ijms20215426
Received: 18 September 2019 / Revised: 28 October 2019 / Accepted: 30 October 2019 / Published: 31 October 2019
(This article belongs to the Section Molecular Pharmacology)
Acylation of antimicrobial peptides mimics the structure of the natural lipopeptide polymyxin B, and increases antimicrobial and endotoxin-neutralizing activities. In this study, the antimicrobial properties of lactoferrin-based LF11 peptides as well as blood compatibility as a function of acyl chain length were investigated. Beyond the classical hemolysis test, the biocompatibility was determined with human leukocytes and platelets, and the influence of antimicrobial peptides (AMPs) on the plasmatic coagulation and the complement system was investigated. The results of this study show that the acylation of cationic peptides significantly reduces blood tolerance. With increasing acyl chain length, the cytotoxicity of LF11 peptides to human blood cells also increased. This study also shows that acylated cationic antimicrobial peptides are inactivated by the presence of heparin. In addition, it could be shown that the immobilization of LF11 peptides leads to a loss of their antimicrobial properties. View Full-Text
Keywords: antimicrobial peptides; inflammation; sepsis; endotoxin; blood compatibility antimicrobial peptides; inflammation; sepsis; endotoxin; blood compatibility
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Harm, S.; Lohner, K.; Fichtinger, U.; Schildböck, C.; Zottl, J.; Hartmann, J. Blood Compatibility—An Important but Often Forgotten Aspect of the Characterization of Antimicrobial Peptides for Clinical Application. Int. J. Mol. Sci. 2019, 20, 5426.

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