Next Article in Journal
The Positive Effects of Grifola frondosa Heteropolysaccharide on NAFLD and Regulation of the Gut Microbiota
Next Article in Special Issue
Endothelial Hyper-Permeability Induced by T1D Sera Can be Reversed by iNOS Inactivation
Previous Article in Journal
Protein Abundance of Clinically Relevant Drug Transporters in The Human Kidneys
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 20
Issue 21
10.3390/ijms20215301
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Association between Nitric Oxide Pathway, Blood Pressure Abnormalities, and Cardiovascular Risk Profile in Pediatric Chronic Kidney Disease

by
Chien-Ning Hsu
1,2,
Pei-Chen Lu
3,
Mao-Hung Lo
3,
I-Chun Lin
3 and
You-Lin Tain
3,*
1
Department of Pharmacy, Kaohsiung Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Kaohsiung 833, Taiwan
2
School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
3
Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Kaohsiung 833, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(21), 5301; https://doi.org/10.3390/ijms20215301
Submission received: 22 September 2019 / Revised: 15 October 2019 / Accepted: 24 October 2019 / Published: 24 October 2019
(This article belongs to the Special Issue Cardiovascular Risk in Children and Adolescents: Focus on Methodological and Pathophysiological Aspects)
Versions Notes

Abstract

Cardiovascular disease (CVD) is common in chronic kidney disease (CKD), while major CV events are rare in young CKD patients. In addition to nitric oxide (NO)-related biomarkers, several surrogate markers have been assessed to stratify CV risk in youth with CKD, including 24-h ambulatory blood pressure monitoring (ABPM), carotid artery intima-media thickness (cIMT), pulse wave velocity (PWV), ABPM-derived arterial stiffness index (AASI), flow-mediated dilatation (FMD), and left ventricular mass index (LVMI). The aim of this study was to identify subclinical CVD through the analysis of indices of CV risk in children and adolescents with CKD. Between 2016 and 2018, the prospective observational study enrolled 125 patients aged 3 to 18 years with G1–G4 CKD stages. Close to two-thirds of young patients with CKD exhibited blood pressure (BP) abnormalities on ABPM. CKD children with abnormal office BP showed lower plasma arginine levels and arginine-to-asymmetric dimethylarginine (ADMA) ratio, but higher ratios of ADMA-to-symmetric dimethylarginine (SDMA) and citrulline-to-arginine. High PWV and AASI, indices of arterial stiffness, both strongly correlated with high BP load. Additionally, LV mass and LVMI exhibited strong correlations with high BP load. Using an adjusted regression model, we observed the citrulline-to-arginine ratio was associated with 24-h systolic and diastolic BP, systolic blood pressure (SBP) load, and diastolic blood pressure (DBP) load. Early assessments of NO-related parameters, BP load abnormalities, arterial stiffness indices, and LV mass will aid in early preventative care toward decreasing CV risk later in life for children and adolescents with CKD.

1. Introduction

Non-communicable diseases (NCDs) present the greatest public health challenges of the 21st century and account for two-thirds of all global deaths [1]. Cardiovascular disease (CVD) alone makes up approximately 50% of all NCD deaths. In general, major CV events tend to occur in adults and are rare in children. However, the stiffening of the arteries can begin in early life. Subclinical CVD presenting in children is not easily detectable by conventional methods [2]. Children with chronic kidney disease (CKD) are at high risk of developing CVD [3]. By the time CKD children require dialysis or kidney transplantation, their CV mortality is 30-fold higher than the general population [4], indicating the need for early identification and prevention.
Surrogate markers have been required to stratify risk for CVD in children. Some biomarkers could evolve to act as surrogates including biomarkers, structural markers, or functional markers. Nitric oxide (NO), a potent vasodilator, plays a relevant role in the regulation of blood pressure (BP) and renal physiology [5]. Emerging evidence supports that NO deficiency is involved in the pathogenesis of hypertension and CKD [6,7,8]. Arginine is a substrate for nitric oxide synthase (NOS) to generate NO and citrulline. Because arginine has multiple metabolic fates and the kidney can use citrulline to make arginine [9,10], there are conflicting findings in the literature according to its plasma level as a biomarker for CKD and hypertension [11,12]. Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are endogenous inhibitors of NOS [13,14]. In adult CKD patients, ADMA and SDMA are independent risk markers for mortality and CVD [15]. Unfortunately, very limited information is available on the simultaneous analysis of these components in the NO pathway to explore their impact on CVD in pediatric CKD [16,17].
Several structural and functional markers have been proposed to stratify the cardiovascular risk in CKD patients, including left ventricular mass [2], 24-h ambulatory blood pressure monitoring (ABPM) [17,18], carotid artery intima-media thickness (cIMT) [19], arterial stiffness indices (e.g., pulse wave velocity [PWV] and ABPM-derived arterial stiffness index [AASI]) [20], and flow-mediated dilatation (FMD) [21]. However, the understanding of these markers in pediatric CKD remains limited. Therefore, the aim of this study was to identify the associations between NO-related biomarkers, BP load, and structural and functional markers in children and adolescents with early-stage CKD.

2. Results

We enrolled a total of 125 children and adolescents with CKD stage G1–G4 in this study. The clinical characteristics of the study participants are presented in Table 1, overall and by office BP. The median age was 9.6 years, 58% were male. Our study cohort had a median estimated glomerular filtration rate (eGFR) of 107 mL/min/1.73 m2, which indicates that most participants had CKD in the early stage. Congenital anomalies of the kidney and urinary tract (CAKUT) were the underlying kidney disease in 64% of patients. The diagnosis of office BP depends on age, gender, and height. Systolic BP (SBP) and/or diastolic BP (DBP) in the 95th percentile or greater was considered abnormal (i.e., hypertension). The adult cut-off was applied at persons aged 16–18 years [22]. Of the 125 patients, 48 cases (38%) were found to have abnormal office BP. As shown in Table 1, children with abnormal office BP had heavier proteinuria, higher plasma levels of total cholesterol, low-density lipoprotein (LDL), and uric acid compared to those with normal office BP.
The plasma levels of NO-related parameters and their combined ratios stratified by the office BP are illustrated in Table 2. The plasma arginine level and arginine-to-ADMA ratio were lower in children with abnormal office BP than in those with normal office BP. Conversely, the ADMA-to-SDMA and citrulline-to-arginine ratios were higher in the abnormal vs. normal office BP group. Plasma levels of citrulline, ADMA, and SDMA, however, were not different between the two groups. We also analyzed the differences of NO-related parameters between patients with CKD stage G2–G4 vs. CKD stage 1. Patients with CKD stage G2–G4 had a higher citrulline level (38 (IQR 29.2–58.3) vs. 30.6 (IQR 24.2–39.8), p = 0.021) while a lower ADMA-to-SDMA ratio (1.37 (IQR 1.03–1.83) vs. 1.74 (IQR 1.55–2.05), p = 0.042) than those with CKD stage G1. No significant differences in arginine, ADMA, SDMA, arginine-to-ADMA ratio, and citrulline-to-arginine ratio were observed between CKD stage G2–G4 and the CKD stage 1 group.
A complete analysis of 24-h ABPM study was feasible in 76 patients aged 6–18 years with CKD (Table 3). After ABPM, we identified: 11 (15%) with systolic BP (SBP) or diastolic BP (DBP) load > 95th percentile at 24-h, 12 (16%) as having daytime hypertension, and 18 patients (24%) as having nighttime hypertension. Additionally, 37 patients (49%) had a BP load ≥ 25% and 32 patients (42%) exhibited nocturnal non-dipping. In the present study, a total of 48 (63%) of CKD children were identified with at least one BP load abnormality on 24-h ABPM. Patients with CKD stage G2–G4 had a higher 24-h SBP (p < 0.001), 24-h DBP (p = 0.043), SBP load (p = 0.004), and DBP load (p = 0.039) than those with CKD stage G1. The rate of patients with abnormal 24-h, daytime BP, nighttime BP, and BP load was higher among patients with CKD stage G2–G4 than those with CKD stage 1. The PWV, an arterial stiffness index, and LV mass were higher in children with CKD stage G2–G4 compared to those with G1. However, cIMT, FMD, AASI, and left ventricular mass index (LVMI) were not different between CKD children with stage G1 and G2–G4. Also, we analyzed the differences in ABPM profile and cardiovascular indices between CKD patients with CAKUT and with non-CAKUT. Nevertheless, we found all cardiovascular indices were comparable between these two groups (All p > 0.05).
As shown in Table 4, we further analyzed indices of cardiovascular structure and function in CKD children by ABPM. We observed that PWV was higher in children with abnormal 24-h, daytime, and nighttime BP, increased BP load, and nocturnal non-dipping than those with normal ABPM profile. Additionally, CKD children with abnormal 24-h, daytime, and nighttime BP in the ABPM profile had a higher AASI compared to those with a normal ABPM profile. Like PWV, LVMI was higher in CKD children with abnormal vs. normal ABPM profile.
Associations between NO-related biomarkers and BP load were further examined in a multivariate linear regression model adjusted for age, sex, and creatinine (Table 5). To specify the exact role of each NO-related biomarker in BP load, a multivariate linear regression model using the stepwise selection was applied for age, sex, and creatinine with some other NO-related biomarkers. In the best predictive model (r = 0.613, p < 0.001), the citrulline-to-arginine ratio was significantly associated with 24-h systolic BP (p = 0.006) controlling for age and creatinine. Also, the citrulline-to-arginine ratio was associated with 24-h diastolic BP (p < 0.001) controlling for creatinine. We also found that both citrulline-to-arginine ratio and citrulline had significant associations with SBP load (p = 0.001 and 0.032, respectively) controlling for creatinine in a predictive model applied for the above-mentioned factors (r = 0.691, p < 0.001). Additionally, a negative association was found for citrulline-to-arginine ratio and DBP load in the adjusted regression model (r = 0.642, p < 0.001).
Correlation analysis of NO-related biomarkers and cardiovascular surrogate markers is detailed in Table 6. We found that arginine was positively correlated with cIMT (r = 0.346, p = 0.003) and PWV (r = 0.258, p = 0.029). Furthermore, citrulline-to-arginine ratio had a negatively correlation with cIMT (r = −0.342, p = 0.003), LV mass (r = −0.267, p = 0.024), and LVMI (r = −0.302, p = 0.011). However, citrulline, ADMA, SDMA, arginine-to-ADMA-ratio, and ADMA-to-SDMA ratio were not correlated with any of the cardiovascular surrogate markers. A multivariate linear regression model was further analyzed to control for the effects of covariates (e.g., age, sex, creatinine) on cardiovascular surrogate markers. When the covariates were taken into account, only arginine had a significant association with cIMT (Beta = 0.384, p = 0.002) in a multivariable regression model applied for age, sex, creatinine, and NO-related biomarkers (r = 0.384, p = 0.002). However, other relationships were no longer significant.

3. Discussion

This study provides insight into a link between NO-related biomarkers, structural and functional markers, and cardiovascular risk in children and adolescents with early-stage CKD. Our major findings are as follows: (1) approximately two-thirds of children and adolescents with CKD stage G1–G4 had BP abnormalities on ABPM; (2) plasma arginine level and arginine-to-ADMA ratio were lower, whereas ADMA-to-SDMA and citrulline-to-arginine ratios were higher in CKD children with abnormal office BP; (3) PWV and LV mass were increased in the case of CKD stage G2–G4 compared to those with stage G1; (4) PWV and LVMI was higher in CKD children with abnormal vs. normal ABPM profile; (5) PWV, LV mass, and LVMI exhibited stronger correlations with elevated BP load than other cardiovascular surrogate markers; and (6) the citrulline-to-arginine ratio was associated with 24-h SBP, 24-h DBP, SBP load, and DBP load in the adjusted regression model.
In line with previous studies in pediatric CKD [3,16,23,24], our study documented an extremely high prevalence of subclinical CVD in CKD children characterized by abnormalities on ABPM, LV mass, and arterial stiffness. In the current study, up to 63% of children with mild to moderate CKD displayed BP abnormalities, despite only 38% of CKD children having abnormal office BP. Our data support the concept that the identification of BP abnormalities not evident in casual BP readings in pediatric CKD has significantly improved with the use of ABPM [25]. We observed several well-known CVD risk factors such as cholesterol, LDL, proteinuria, and uric acid were correlated to CKD children with abnormal office BP. Among them, uric acid showed a strong positive correlation with elevated BP load. Additionally, we observed that PWV and AASI, indices of arterial stiffness, both are strongly correlated with uric acid. These findings are supported by a previous study of adult patients with CKD, in whom a worse arterial stiffness is related to hyperuricemia-induced hypertension [26]. Since uric acid is considered not only a CVD risk factor but also a potential therapeutic target in CKD [27], more studies are needed to clarify whether early targeting on uric acid can prevent the development of CVD in children with CKD, even in an early stage.
Although most evidence indicates NO deficiency in CKD and hypertension [6,7,8], some studies reported that NO synthesis was increased in patients with CKD [28]. In the current study, CKD children with hypertension exhibited a low plasma arginine level, low arginine-to-ADMA ratio, and high ADMA-to-SDMA ratio. As arginine is the substrate for NOS and arginine-to-ADMA ratio is referred to as an index of NO bioavailability [29], our results suggest NO deficiency is related to the development of hypertension in children and adolescents with early-stage CKD. In the present study, FMD was assessed to evaluate endothelial dysfunction, a condition that entails less local availability of NO [21]. However, we did not observe the associations between FMD and NO-related biomarkers, BP load, and other CV surrogate markers. It is possible that the small number of patients included may not reach sufficient power to determine small differences in early stage of CKD. Although a previous report demonstrated that plasma ADMA and SDMA levels positively correlate with BP load in children with CKD [17], such findings are not supported by the present data. Unlike ADMA, SDMA is excreted by the kidneys only. A high ADMA-to-SDMA ratio could be suggestive of reduced ADMA excretion or metabolism [30]. Thus, ADMA-to-SDMA ratio may provide information about the dimethylarginine dimethylaminohydrolase (DDAH) activity because only ADMA is metabolized by the enzyme [14]. We observed that hypertension is related to a higher ADMA-to-SDMA ratio in CKD children. In agreement with a previous study showing that increased DDAH activity can reduce ADMA and prevent hypertension in young spontaneously hypertensive rats [31], our data suggest decreased DDAH activity might be involved in the development of hypertension. When the covariates were taken into account, the citrulline-to-arginine ratio was the only NO-related marker, which could well explain BP load in the adjusted regression model. In patients with CKD, a decreased renal uptake of citrulline and its conversion to arginine can result in a high plasma citrulline-to-arginine ratio. We observed that citrulline and citrulline-to-arginine ratio both were negatively associated with SBP load. Given that CKD children with a high BP load had a low citrulline-to-arginine ratio, it is possible a high conversion rate of citrulline to arginine generated NO as a compensatory response in the pre-hypertensive stage. In adult CKD patients, higher plasma ADMA and SDMA levels were reported in patients with more advanced CKD [32]. These findings are not supported by the results of the present analyses, which demonstrate that almost all NO-related parameters did not differ in CKD children with stage G2–G4 vs. stage G1. Thus, the roles of NO-related biomarkers in CKD progression in children await further clarification.
As expected, the severity of CKD is associated with certain markers of adverse CV health, like PWV and LV mass in the current study. Although PWV has been used in children, age-specific reference ranges are still unavailable [2]. In the present study, PWV was increased in children with abnormal 24-h, daytime, and nighttime BP, increased BP load, and nocturnal non-dipping. Additionally, PWV was strongly correlated with 24-h SBP, 24-h DBP, SBP load, and DBP load. Like PWV, the AASI is an indirect arterial stiffness index [33]. Our results demonstrated that high AASI is related to abnormal 24-h, daytime, and nighttime BP in the ABPM profile in children with CKD. The present results are in agreement with the previous study in that arterial stiffness worsens in CKD patients with masked hypertension [34]. In addition to PWV and AASI, cIMT might also have a smaller role in contributing to BP load. We found a weaker correlation between cIMT and high BP load than indices of arterial stiffness. It is possible that arterial stiffness enables evaluation of arterial dysfunction, which may precede structural vascular remodeling evaluated by cIMT. Left ventricular hypertrophy (LVH), an index of target organ damage, poses an increased risk for CVD in patients with CKD [35]. In keeping with previous studies showing that ABPM correlates with LV hypertrophy in CKD children [18,36], we found LV mass and LVMI both strongly correlated with systolic and diastolic BP load on ABPM.
Our study was not without its limitations. First, additional assessments of the above-mentioned CV markers with longer follow-ups are essential in view of the long-term nature of childhood CKD. Second, there are no reference values established for PWV, AASI, cIMT, and LVMI to define a cut-off point between healthy and CKD children [2]. Third, there might be an ethnic difference as we used BP nomograms and ABPM reference values from studies in white people [37,38]. Fourth, two new hypertension guidelines in children and adolescents have been released recently [22,39] and result in discrepancies on BP nomograms, the definition of BP categories based on percentiles, as well as the adult cut-off age. Thus, a plan of action needs to evaluate the impact of different guidelines that have introduced redefinitions of BP categories on the above-mentioned CV markers and CV outcomes. Last, the small number of patients included might not reveal a true relationship. Larger numbers of CKD children recruited via multi-center cohorts may be warranted.

4. Materials and Methods

4.1. Study Population

The prospective cohort study was approved by the Institution Review Board and Ethics Committee of Chang Gung Medical Foundation, Taoyuan, Taiwan (Permit number: 201601181A3; approval date: 30 December 2016) and adherent to the principle of the 1964 Declaration of Helsinki and its later amendments. All participants gave written informed consent. The present study includes 125 participants who contributed at least one visit to a pediatric nephrology clinic in a Taiwan medical center between December 2016 and October 2018, while they were between 3 to 18 years of age with CKD. CKD is defined as kidney damage or decreased kidney function over at least three months [40]. Kidney damage refers to structural abnormalities or functional abnormalities, whether established via renal biopsy or imaging studies, or inferred from markers such as urinary sediment abnormalities or proteinuria. Renal function was determined by the estimated glomerular filtration rate (eGFR) using the Schwartz formula according to body height and serum creatinine level [41]. Individuals who were excluded from the study were those who: (1) had a documented pregnancy; (2) had a congenital heart disease; (3) had an eGFR < 15 mL/min/1.73 m2, or were on dialysis maintenance or had ever received a renal transplantation; or (4) were unable to follow-up protocol or cooperate with the assessment. All participants were categorized to eGFR categories: G1 (eGFR ≥ 90 mL/min/1.73 m2), G2 (eGFR 60–89 mL/min/1.73 m2), G3 (eGFR 30–59 mL/min/1.73 m2), or G4 (eGFR 15–30 mL/min/1.73 m2). The analysis was restricted to children with a baseline eGFR > 15ml/min/1.73m2, who measured renal and cardiovascular parameters described in the following section. The causes of kidney disease were divided into two categories: Congenital anomalies of the kidney and urinary tract (CAKUT), or non-CAKUT. CAKUT structural anomalies range from renal agenesis, kidney hypoplasia, kidney dysplasia, multi-cystic kidney dysplasia, horseshoe kidney, duplex collecting system, posterior urethral valves, and ureter abnormalities [42].

4.2. Biochemical Analysis

Fasting plasma specimens, spot urine, and fecal samples were aliquoted and stored at –80 °C until analysis. Blood urea nitrogen, creatinine, uric acid, glucose, total cholesterol, LDL, triglyceride, sodium, potassium, calcium, phosphate, hemoglobin, and urine total protein-to-creatinine ratio were measured by the hospital central laboratory. We directed the families to prevent their children from having an excessive intake of foods rich with arginine and citrulline (e.g., peanut, gelatin, and watermelon) for 1 week before blood and urine sampling.

4.3. Office BP and 24-h ABPM

BP measurements were taken at the clinic visit. The participant rested quietly for 5 min in a seated position. Three BP recordings were obtained 30 s apart by experienced specialist nurses using a standard mercury sphygmomanometer (Philips MP5, Boeblingen, Germany). A cuff with a bladder sized by a 1:2 width to length ratio according to the size of the arm was chosen. The mean value was used as the participant’s office BP for analysis. The 24-h ABPM data were collected for subjects aged 6–18 years using an Oscar II monitoring device (SunTech Medical, Morrisville, NC, USA) handled by a trained specialist nurse as previously reported [42]. Briefly, the ABPM was set to record the BP and pulse rate at 20-min intervals over 24-h. The subjects and their parents were asked to keep a diary of sleeping and waking times, as well as activities that may influence BP measurements, including exercise and stressful situations. Only measurements with a systolic BP of 50–200 mmHg, a diastolic BP of 30–100 mmHg, and a heart rate of 30–200 beats per min were accepted as valid and included in analysis. If more than 25% of an individual’s recordings were outside these valid ranges, then that individual was excluded from further analysis.
An abnormal ABPM profile was determined based on: (1) awake, asleep, systolic, or diastolic BP loads exceeding the 95th percentile based on gender and height using ABPM reference data [38]; (2) awake, asleep, systolic or diastolic BP load of 25% or greater; and (3) asleep decrease of BP load by less than 10% compared with average awake BP load. Additionally, we calculated the AASI as one minus the slope of the DBP reading over the SBP reading [34].

4.4. Carotid Ultrasonography and Echocardiography

The ultrasound studies were performed by two experienced pediatricians (P.-C.L. and I.-C.L.) as previously reported [16]. Patients were in the supine position for at least 10-min in a quiet room prior to examination. The patient’s chin was hyperextended and turned toward the contralateral side at about a 45-degree angle from the midline. The mid common carotid artery was imaged using a 5- to 12-MHz linear array transducer (Aloka Co., Tokyo, Japan). We used the distance between the leading edges of the luminal-intimal interface and the medial-adventitial interface for the measurement of cIMT. The cIMT was measured during end-diastole as determined by the R wave on the electrocardiogram. These images were obtained by the ProSound α7 ultrasound coupled to computer-assisted analysis software (ProSound α7, e-TRACKING system; Aloka Co., Tokyo, Japan). PWV was determined by echo-tracking methods (e-TRACKING system; Aloka Co., Tokyo, Japan). Endothelial function was analyzed by measuring FMD, defined as the maximal percentage change in right brachial arterial diameter in response to reactive hyperemia induced by inflating a BP cuff to >200 mmHg around the forearm for 5 min. Brachial artery diameter was measured in longitudinal section with a UST-5412 linear probe and a ProSound a7 ultrasound system coupled to computer-assisted analysis software, as previously described [16]. Each study included a resting scan and reactive hyperemia scan recorded 30–60 s after the cuff pressure was released, which occluded arterial blood flow for 5 min. Additionally, all participants underwent a transthoracic echocardiographic examination with commercially available machines (Philips IE33 system, Philips, Bothell, WA, USA). The left ventricular mass (LVM) was calculated using images obtained in the parasternal long-axis or short-axis view of the left ventricle by M-mode echocardiography. The LVM index (LVMI) was obtained by indexing LVM to height2.7 [43].

4.5. HPLC Analysis

Plasma levels of citrulline, arginine, ADMA, and SDMA were determined using high-performance liquid chromatography (HPLC: HP series 1100, Agilent Technologies, Inc, Santa Clara, CA, USA). O-phthaldialdehyde 3-mercaptopropionic acid (OPA-3MPA) was used for the fluorescent derivatization [44]. The standards contained arginine and citrulline at 1–100 μmol/L. For ADMA and SDMA, the standards were set at 0.5–5 μmol/L.

4.6. Statistical Analysis

Data were expressed as median (25th percentile–75th percentile) or n (%), as appropriate. Group differences were analyzed by Mann–Whitney U-test or Chi-square test, as appropriate. The associations between variables were assessed using Spearman’s rank correlations. A linear regression model was performed followed by the stepwise multivariable analyses integrating relevant parameters to explain BP load. A value of p < 0.05 was considered statistically significant. Statistical Package for the Social Sciences (SPSS) software 14.0 (Chicago, IL, USA) was used.

5. Conclusions

In conclusion, a significant proportion of children with CKD, even in early-stage, exhibit BP abnormalities and an adverse CV risk profile. Although the mechanism underlying the NO pathway and CVD in CKD children has not yet been completely clarified, our results cast a new light on the link between NO pathway, BP load, and arterial stiffness in children with CKD. Early detection of NO-related biomarkers and structural and functional surrogate markers may assist in advancing knowledge of CVD and initiating treatment at an early stage with respect to improving CV outcome in at-risk children with CKD.

Author Contributions

C.-N.H. contributed to concept generation, data interpretation, drafting of the manuscript, critical revision of the manuscript, and approval of the article; P.-C.L. contributed to data interpretation, critical revision of the manuscript, and approval of the article; M.-H.L. contributed to data interpretation, critical revision of the manuscript, and approval of the article; I.-C.L.contributed to methodology and approval of the article; Y.-L.T. contributed to concept generation, data interpretation, drafting of the manuscript, critical revision of the manuscript, and approval of the article.

Funding

This work was supported by grants CORPG8F1031, CORPG8F1032, and CORPG8F1033 from the Kaohsiung Chang Gung Memorial Hospital, Taiwan.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. James, S.L.; Abate, D.; Abate, K.H.; Abay, S.M.; Abbafati, C.; Abbasi, N.; Abdollahpour, I. Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990–2017: A systematic analysis for the Global Burden of Disease Study 2017. Lancet 2018, 392, 1789–1858. [Google Scholar] [CrossRef]
  2. Urbina, E.M.; Williams, R.V.; Alpert, B.S.; Collins, R.T.; Daniels, S.R.; Hayman, L.; Jacobson, M.; Mahoney, L.; Mietus-Snyder, M.; Rocchini, A.; et al. Noninvasive assessment of subclinical atherosclerosis in children and adolescents: Recommendations for standard assessment for clinical research: A scientific statement from the American Heart Association. Hypertension 2009, 54, 919–950. [Google Scholar] [CrossRef] [PubMed]
  3. Mitsnefes, M.M. Cardiovascular disease in children with chronic kidney disease. J. Am. Soc. Nephrol. 2012, 23, 578–585. [Google Scholar] [CrossRef] [PubMed]
  4. McDonald, S.P.; Craig, J.C. Long-term survival of children with end-stage renal disease. N. Eng. J. Med. 2004, 350, 2654–2662. [Google Scholar] [CrossRef]
  5. Hsu, C.N.; Tain, Y.L. Regulation of nitric oxide production in the developmental programming of hypertension and kidney disease. Int. J. Mol. Sci. 2019, 20, 681. [Google Scholar] [CrossRef]
  6. Wilcox, C.S. Oxidative stress and nitric oxide deficiency in the kidney: A critical link to hypertension? Am. J. Physiol. Regul. Integr. Comp. Physiol. 2005, 89, R913–R915. [Google Scholar] [CrossRef]
  7. Baylis, C. Nitric oxide synthase derangements and hypertension in kidney disease. Curr. Opin. Nephrol. Hypertens. 2012, 21, 1–6. [Google Scholar] [CrossRef]
  8. Török, J. Participation of nitric oxide in different models of experimental hypertension. Physiol. Res. 2008, 57, 813–825. [Google Scholar]
  9. Wu, G.; Morris, S.M., Jr. Arginine metabolism: Nitric oxide and beyond. Biochem. J. 1998, 336, 1–17. [Google Scholar] [CrossRef]
  10. Hsu, C.N.; Tain, Y.L. Impact of arginine nutrition and metabolism during pregnancy on Offspring outcomes. Nutrients 2019, 11, 1452. [Google Scholar] [CrossRef]
  11. Levillain, O.; Parvy, P.; Hassler, C. Amino acid handling in uremic rats: Citrulline, a reliable marker of renal insufficiency and proximal tubular dysfunction. Metabolism 1997, 46, 611–618. [Google Scholar] [CrossRef]
  12. Tizianello, A.; De Ferrari, G.; Garibotto, G.; Gurreri, G.; Robaudo, C. Renal metabolism of amino acids and ammonia in subjects with normal renal function and in patients with chronic renal insufficiency. J. Clin. Invest. 1980, 65, 1162–1173. [Google Scholar] [CrossRef] [PubMed]
  13. Leone, A.; Moncada, S.; Vallance, P.; Calver, A.; Collier, J. Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 1992, 339, 572–575. [Google Scholar] [CrossRef]
  14. Tain, Y.L.; Hsu, C.N. Toxic dimethylarginines: Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Toxins 2017, 9, 92. [Google Scholar] [CrossRef] [PubMed]
  15. Schlesinger, S.; Sonntag, S.R.; Lieb, W.; Maas, R. Asymmetric and symmetric dimethylarginine as risk markers for total mortality and cardiovascular outcomes: A systematic review and meta-analysis of prospective studies. PLoS ONE 2016, 11, 0165811. [Google Scholar] [CrossRef]
  16. Lin, I.C.; Hsu, C.N.; Lo, M.H.; Chien, S.J.; Tain, Y.L. Low urinary citrulline/arginine ratio associated with blood pressure abnormalities and arterial stiffness in childhood chronic kidney disease. J. Am. Soc. Hypertens. 2016, 10, 115–123. [Google Scholar] [CrossRef]
  17. Brooks, E.R.; Langman, C.B.; Wang, S.; Price, H.E.; Hodges, A.L.; Darling, L.; Yang, A.Z.; Smith, F.A. Methylated arginine derivatives in children and adolescents with chronic kidney disease. Pediatr. Nephrol. 2009, 24, 129–134. [Google Scholar] [CrossRef]
  18. Mitsnefes, M.; Flynn, J.; Cohn, S.; Samuels, J.; Blydt-Hansen, T.; Saland, J.; Kimball, T.; Furth, S.; Warady, B.; CKiD Study Group. Masked hypertension associates with left ventricular hypertrophy in children with CKD. J. Am. Soc. Nephrol. 2010, 21, 137–144. [Google Scholar] [CrossRef]
  19. Brady, T.M.; Schneider, M.F.; Flynn, J.T.; Cox, C.; Samuels, J.; Saland, J.; White, C.T.; Furth, S.; Warady, B.A.; Mitsnefes, M. Carotid intima-media thickness in children with CKD: Results from the CKiD study. Clin. J. Am. Soc. Nephrol. 2012, 7, 1930–1937. [Google Scholar] [CrossRef]
  20. Taal, M.W. Arterial stiffness in chronic kidney disease: An update. Curr. Opin. Nephrol. Hypertens. 2014, 23, 169–173. [Google Scholar] [CrossRef]
  21. Deanfield, J.E.; Halcox, J.P.; Rabelink, T.J. Endothelial function and dysfunction: Testing and clinical relevance. Circulation 2007, 115, 1285–1295. [Google Scholar] [CrossRef] [PubMed]
  22. Lurbe, E.; Agabiti-Rosei, E.; Cruickshank, J.K.; Dominiczak, A.; Erdine, S.; Hirth, A.; Invitti, C.; Litwin, M.; Mancia, G.; Pall, D.; et al. 2016 European Society of Hypertension guidelines for the management of high blood pressure in children and adolescents. J. Hypertens. 2016, 34, 1887–1920. [Google Scholar] [CrossRef] [PubMed]
  23. Wong, C.J.; Moxey-Mims, M.; Jerry-Fluker, J.; Warady, B.A.; Furth, S.L. CKiD (CKD in children) prospective cohort study: A review of current findings. Am. J. Kidney Dis. 2012, 60, 1002–1011. [Google Scholar] [CrossRef] [PubMed]
  24. Chou, H.H.; Lin, C.Y.; Chiou, Y.H.; Tain, Y.L.; Wang, Y.F.; Wang, H.H.; Chiou, Y.Y. Clinical characteristics and prevalence of complications of chronic kidney disease in children: The Taiwan Pediatric Renal Collaborative study. Pediatr. Nephrol. 2016, 31, 1113–1120. [Google Scholar] [CrossRef] [PubMed]
  25. Graves, J.W.; Althaf, M.M. Utility of ambulatory blood pressure monitoring in children and adolescents. Pediatr. Nephrol. 2006, 21, 1640–1652. [Google Scholar] [CrossRef] [PubMed]
  26. Tomiyama, H.; Shiina, K.; Vlachopoulos, C.; Iwasaki, Y.; Matsumoto, C.; Kimura, K.; Fujii, M.; Chikamori, T.; Yamashina, A. Involvement of arterial stiffness and Inflammation in hyperuricemia-related development of hypertension. Hypertension 2018, 72, 739–745. [Google Scholar] [CrossRef]
  27. Fathallah-Shaykh, S.A.; Cramer, M.T. Uric acid and the kidney. Pediatr. Nephrol. 2004, 29, 999–1008. [Google Scholar] [CrossRef]
  28. Lau, T.; Owen, W.; Yu, Y.M.; Noviski, N.; Lyons, J.; Zurakowski, D.; Tsay, R.; Ajami, A.; Young, V.R.; Castillo, L. Arginine, citrulline, and nitric oxide metabolism in end-stage renal disease patients. J. Clin. Invest. 2000, 105, 1217–1225. [Google Scholar] [CrossRef]
  29. Bode-Boger, S.M.; Scalera, F.; Ignarro, L.J. The l-arginine paradox: Importance of the l-arginine/asymmetrical dimethylarginine ratio. Pharm. Ther. 2007, 114, 295–306. [Google Scholar] [CrossRef]
  30. Bassareo, P.P.; Fanos, V.; Puddu, M.; Flore, G.; Mercuro, G. Advanced intrauterine growth restriction is associated with reduced excretion of asymmetric dimethylarginine. Early Hum. Dev. 2014, 90, 173–176. [Google Scholar] [CrossRef]
  31. Tain, Y.L.; Huang, L.T.; Lin, I.C.; Lau, Y.T.; Lin, C.Y. Melatonin prevents hypertension and increased asymmetric dimethylarginine in young spontaneous hypertensive rats. J. Pineal Res. 2010, 49, 390–398. [Google Scholar] [CrossRef] [PubMed]
  32. Emrich, I.E.; Zawada, A.M.; Martens-Lobenhoffer, J.; Fliser, D.; Wagenpfeil, S.; Heine, G.H.; Bode-Böger, S.M. Symmetric dimethylarginine (SDMA) outperforms asymmetric dimethylarginine (ADMA) and other methylarginines as predictor of renal and cardiovascular outcome in non-dialysis chronic kidney disease. Clin. Res. Cardiol. 2018, 107, 201–213. [Google Scholar] [CrossRef] [PubMed]
  33. Drawz, P.E.; Alper, A.B.; Anderson, A.H.; Brecklin, C.S.; Charleston, J.; Chen, J.; Deo, R.; Fischer, M.J.; He, J.; Hsu, C.Y.; et al. Masked hypertension and elevated nighttime blood pressure in CKD: Prevalence and association with target organ damage. Clin. J. Am. Soc. Nephrol. 2016, 11, 642–652. [Google Scholar] [CrossRef] [PubMed]
  34. Kollias, A.; Stergiou, G.S.; Dolan, E.; O’Brien, E. Ambulatory arterial stiffness index: A systematic review and meta-analysis. Atherosclerosis 2012, 224, 291–301. [Google Scholar] [CrossRef] [PubMed]
  35. K/DOQI Workgroup. K/DOQI clinical practice guidelines for cardiovascular disease in dialysis patients. Am. J. Kidney Dis. 2005, 45, S1–S153. [Google Scholar]
  36. Gupta, D.; Chaturvedi, S.; Chandy, S.; Agarwal, I. Role of 24-h ambulatory blood pressure monitoring in children with chronic kidney disease. Indian, J. Nephrol. 2015, 25, 355–361. [Google Scholar] [CrossRef]
  37. National High Blood Pressure Education Program. The Fourth Report on the Diagnosis, Evaluation, and Treatment of High Blood Pressure in Children and Adolescents. 2004. Available online: https://www.nhlbi.nih.gov › docs › resources › heart › hbp_ped (accessed on 1 September 2019).
  38. Wuhl, E.; Witte, K.; Soergelm, M.; Mehls, O.; Schaefer, F.; German Working Group on Pediatric Hypertension. Distribution of 24-h ambulatory blood pressure in children: Normalized reference values and role of body dimensions. J. Hypertens. 2002, 20, 1995–2007. [Google Scholar] [CrossRef]
  39. Flynn, J.T.; Kaelber, D.C.; Baker-Smith, C.M.; Blowey, D.; Carroll, A.E.; Daniels, S.R.; de Ferranti, S.D.; Dionne, J.M.; Falkner, B.; Flinn, S.K.; et al. Clinical practice guideline for screening and management of high blood pressure in children and adolescents. Pediatrics 2017, 140, e20171904. [Google Scholar] [CrossRef]
  40. Levin, A.; Stevens, P.E.; Bilous, R.W.; Coresh, J.; De Francisco, A.L.M.; De Jong, P.E.; Griffith, K.E.; Hemmelgarn, B.R.; Iseki, K.; Lamb, E.J. KDIGO 2012 clinical practice guideline for the evaluation and management of chronic kidney disease. Kidney Int. Suppl. 2013, 3, 1–150. [Google Scholar]
  41. Schwartz, G.J.; Muñoz, A.; Schneider, M.F.; Mak, R.H.; Kaskel, F.; Warady, B.A.; Furth, S.L. New equations to estimate GFR in children with CKD. J. Am. Soc. Nephrol. 2009, 20, 629–637. [Google Scholar] [CrossRef]
  42. Renkema, K.Y.; Winyard, P.J.; Skovorodkin, I.N.; Levtchenko, E.; Hindryckx, A.; Jeanpierre, C.; Weber, S.; Salomon, R.; Antignac, C.; Vainio, S.; et al. Novel perspectives for investigating congenital anomalies of the kidney and urinary tract (CAKUT). Nephrol. Dial. Transplant. 2011, 26, 3843–3851. [Google Scholar] [CrossRef] [PubMed]
  43. Daniels, S.R.; Kimball, T.R.; Morrison, J.A.; Khoury, P.; Meyer, R.A. Indexing left ventricular mass to account for differences in body size in children and adolescents without cardiovascular disease. Am. J. Cardiol. 1995, 76, 699–701. [Google Scholar] [CrossRef]
  44. Chien, S.J.; Lin, I.C.; Hsu, C.N.; Lo, M.H.; Tain, Y.L. Homocysteine and arginine-to-asymmetric dimethylarginine ratio associated with blood pressure abnormalities in children with early chronic kidney disease. Circ. J. 2015, 79, 2031–2037. [Google Scholar] [CrossRef] [PubMed]
Table 1. Patient characteristics in children and adolescents with CKD.
Table 1. Patient characteristics in children and adolescents with CKD.
All PatientsOffice BP
NormalAbnormal
CharacteristicN = 125N = 77N = 48
Age, years9.6 (5.3–14.4)10 (6.4–14.2)8.7 (4.8–15.7)
Male72 (57.6%)41 (53.2%)31 (64.6%)
CKD staging
Stage G191 (72.8%)59 (76.6%)32 (66.7%)
Stage G222 (17.6%)12 (15.6%)10 (20.8%)
Stage G310 (8%)6 (7.8%)4 (8.3%)
Stage G42 (1.6%)0 (0%)2 (4.2%)
CAKUT80 (64%)51 (66.2%)29 (60.4%)
Body height, percentile50 (20–75)50 (25–75)25 (15–75)
Body weight, percentile50 (15–85)50 (15–75)63 (15–85)
Systolic blood pressure, mmHg109 (100–120)103 (98–114)119 (109–136) *
Diastolic blood pressure, mmHg69 (63–77)64 (60–71)77 (68–83) *
Body mass index, kg·m−217.6 (15.6–20.7)17.1 (15.2–20.3)18 (16.1–22.3)
Blood urea nitrogen, mg/dL12 (10–15)12 (10–14)13 (10–17)
Creatinine, mg/dL0.5 (0.42–0.69)0.51 (0.44–0.67)0.5 (0.37–0.7)
eGFR, mL/min/1.73 m2107 (87–125)110 (91–125)102 (83–126)
Urine total protein-to-creatinine ratio, mg/g69 (40–283)61 (38–169)128 (43–1424) *
Hemoglobin, g/dL13.4 (12.6–14.2)13.4 (12.6–14.2)13.4 (12.4–14.6)
Hematocrit, %39.7 (37.3–41.8)39.6 (37.3–41.8)40 (37.3–42.6)
Total cholesterol, mg/dL165 (146–190)160 (140–179)177 (154–202) *
LDL, mg/dL87 (71–105)81 (65–100)94 (78–128) *
Triglyceride, mg/dL67 (51–103)62 (50–92)72 (51–142)
Fasting glucose, mg/dL87 (83–92)87 (83–91)87 (83–94)
Uric acid, mg/dL5.3 (4.1–6.3)4.9 (4–6.1)5.8 (5–7) *
Sodium, mEq/L141 (140–142)141 (140–142)141 (140–142)
Potassium, mEq/L4.4 (4.2–4.6)4.4 (4.2–4.5)4.4 (4.1–4.6)
Calcium, mg/dL9.7 (9.3–9.9)9.7 (9.4–9.9)9.6 (9.1–10)
Phosphate, mg/dL4.9 (4.5–5.3)4.9 (4.5–5.3)5 (4.4–5.4)
Ca × P product, mg2/dL247.5 (41.7–51.8)47 (41.8–51.8)47.6 (41.4–51.8)
Data are given as median (25th percentile–75th percentile) or n (%) as appropriate. * p < 0.05 children (age < 12 years old) vs. adolescent (age > 12 years old) by the Chi-square test or Mann–Whitney U-test. BP = blood pressure; CAKUT = Congenital anomalies of the kidney and urinary tract; eGFR = estimated glomerular filtration rate; LDL = low-density lipoprotein.
Table 2. Plasma levels of NO pathway components in children and adolescents with CKD.
Table 2. Plasma levels of NO pathway components in children and adolescents with CKD.
Office BPNormal Abnormal
N = 74N = 47
Citrulline, μM36 (25.7–46.1)37.6 (27.5–47.2)
Arginine, μM92.2 (71–109)78.5 (57.5–98.9) *
ADMA, μM1.05 (0.7–1.33)1.1 (0.8–1.3)
SDMA, μM0.7 (0.6–0.93)0.6 (0.5–0.8)
Arginine-to-ADMA ratio, μM/μM89.5 (54.2–141.4)74.8 (57.2–93.3) *
ADMA-to-SDMA ratio, μM/μM1.5 (1–1.91)1.71 (1.43–2.33) *
Citrulline-to-Arginine ratio, μM/μM0.4 (0.3–0.58)0.51 (0.39–0.68) *
Data are given as median (25th percentile–75th percentile). * p < 0.05 by the Mann-Whitney U-test. NO = nitric oxide; SCFAs = short chain fatty acids; ADMA = asymmetric dimethylarginine; SDMA = symmetric dimethylarginine.
Table 3. Cardiovascular characteristics in children and adolescents with CKD.
Table 3. Cardiovascular characteristics in children and adolescents with CKD.
CKD StageG1G2–G4Total
24-h ABPM N = 50 N = 26N = 76
24-h systolic BP109 (101–116)120 (112–135) *112 (103–121)
24-h diastolic BP63 (58–66)65 (61–72) *64 (59–68)
SBP load2.5 (0–15)21 (2.8–45.3) *4 (2–27.8)
DBP load2 (0–7.2)5 (0–16.3) *2 (0–8)
Abnormal ABPM profile (with any of the following abnormalities) 26 (52%)22 (85%) *48 (63%)
Average 24-h BP > 95th percentile4 (8%)7 (27%) *11 (15%)
Average daytime BP > 95th percentile4 (8%)8 (31%) *12 (16%)
Average nighttime > 95th percentile8 (16%)10 (39%) *18 (24%)
BP load ≥ 25%17 (34%)20 (77%) *37 (49%)
Nocturnal decrease of BP < 10%19 (38%)13 (50%)32 (42%)
cIMT, mm0.3 (0.3–0.4)0.3 (0.3–0.4)0.3 (0.3–0.4)
PWV, m/s3.8 (3.4–4.2)4 (3.6–4.8) *3.85 (3.53–4.38)
FMD, %8.3 (3.4–12.9)4.6 (2.6–15.4)7.8 (3.2–12.9)
AASI0.37 (0.22–0.43)0.39 (0.33–0.48)0.36 (0.24–0.44)
LV mass, g87.5 (66.5–115)123 (73.7–159) *94.9 (66.5–127)
LVMI, g/m2.730.7 (25.6–37.2)31 (28.6–39)30.9 (26.1–38.2)
Data are given as median (25th percentile–75th percentile) or n (%) as appropriate. * p < 0.05 by the Chi-square test. ABPM = 24-h ambulatory blood pressure monitoring. cIMT = carotid artery intima-media thickness. PWV = pulse wave velocity. FMD = flow-mediated dilatation. AASI = ABPM-derived arterial stiffness index. LVMI = left ventricular mass index.
Table 4. Cardiovascular parameters vs. ABPM profile in children and adolescents with CKD.
Table 4. Cardiovascular parameters vs. ABPM profile in children and adolescents with CKD.
BPcIMT, mmPWV, m/sFMD, %AASILVMI, g/m2.7
24-h BP
Abnormal0.4 (0.3–0.4) 4.1 (4–5) *7.4 (3.8–14.7) 0.44 (0.36–0.54) *40.1 (28.9–47.2) *
Normal0.3 (0.3–0.4)3.8 (3.5–4.3)7.8 (2.9–12.7) 0.34 (0.24–0.43)30.6 (26–36.2)
Daytime BP
Abnormal0.4 (0.3–0.4)4.2 (4–5) *6 (3.2–13.8)0.42 (0.36–0.54) *39.1 (29.5–46.9) *
Normal0.3 (0.3–0.4)3.8 (3.4–4.3)7.8 (3–13)0.34 (0.24–0.43)30.6 (26–35.2)
Nighttime BP
Abnormal0.4 (0.3–0.4)4.2 (3.9–4.8) * 4.6 (3.2–12.9) 0.42 (0.33–0.53) *37 (30.7–47.2) *
Normal0.3 (0.3–0.4)3.8 (3.4–4.2)8.1 (2.8–13.2) 0.34 (0.23–0.42)30.4 (25.8–35.1)
BP load
Abnormal0.4 (0.3–0.4)4.1 (3.6–4.8) * 5 (3–11.7)0.38 (0.24–0.46)32.9 (29.2–40.7) *
Normal0.3 (0.3–0.4)3.8 (3.4–4.1)8.7 (4–15.3) 0.34 (0.24–0.43)29.7 (25.6–32.9)
Night dipping
Abnormal0.3 (0.3–0.4)3.9 (3.4–4.3) *6.5 (3.3–10.7) 0.37 (0.24–0.49)31.1 (29.1–41) *
Normal0.3 (0.3–0.4)3.8 (3.6–4.5)9.3 (2.5–15.7) 0.34 (0.24–0.42)30.6 (25.9–36.8)
ABPM profile
Abnormal0.4 (0.3–0.4)4 (3.6–4.6) *7.2 (3.3–11.7) 0.39 (0.24–0.46)31.8 (28.1–40) *
Normal0.3 (0.3–0.4)3.8 (3.4–4.2) 9.3 (2.2–16.5) 0.31 (0.24–0.42)28.8 (25.7–32.9)
Data are given as median (25th percentile–75th percentile). * p < 0.05 by the Mann-Whitney U-test. cIMT = carotid artery intima-media thickness. PWV = pulse wave velocity. FMD = flow-mediated dilatation. AASI = ABPM-derived arterial stiffness index. LVMI = left ventricular mass index.
Table 5. Adjusted regression model estimates (betas, p-values) of the association between BP load and NO-related biomarkers in children with CKD.
Table 5. Adjusted regression model estimates (betas, p-values) of the association between BP load and NO-related biomarkers in children with CKD.
Dependent ValuableExplanatory ValuableAdjusted aModel
Betap-Valuerp-Value
24-h systolic BPCitrulline-to-arginine ratio−0.2880.0060.613<0.001
24-h diastolic BPCitrulline-to-arginine ratio−0.409<0.0010.625<0.001
SBP loadCitrulline-to-arginine ratio−0.3310.0010.691<0.001
Citrulline−0.2400.032
DBP loadCitrulline-to-arginine ratio−0.2890.0040.642<0.001
a Adjusted for age, sex, creatinine, and NO-related biomarkers.
Table 6. Correlation between NO-related biomarkers and cardiovascular surrogate markers in children with CKD stage G1–G3.
Table 6. Correlation between NO-related biomarkers and cardiovascular surrogate markers in children with CKD stage G1–G3.
CV MarkerscIMT PWV FMD AASI LV MassLVMI
rprprprprprp
Citrulline−0.0280.8130.0350.7710.0270.8350.0920.443−0.1270.291−0.1430.233
Arginine0.3460.003 *0.2580.029 *0.0260.8450.210.079−0.1970.0990.210.078
ADMA0.0840.483−0.0740.537−0.1770.1730.1180.328−0.0030.978−0.1280.287
SDMA−0.1220.307−0.0510.6730.0760.5610.0390.7450.0790.515−0.210.079
Arginine-to-ADMA 0.2240.0590.2320.050.0830.5250.0740.5370.1940.1040.2090.08
ADMA-to-SDMA0.0060.961−0.0090.942−0.0890.494−0.0090.942−0.1270.2910.0460.688
Citrulline-to-Arginine−0.3420.003 *−0.1590.1810.0010.991−0.0550.648−0.2670.024 *−0.3020.011 *
cIMT = carotid artery intima-media thickness; PWV = pulse wave velocity; FMD = flow-mediated dilatation; AASI = ABPM-derived arterial stiffness index; LV mass = Left ventricular mass; LVMI = Left ventricular mass index. * p < 0.05 by the Spearman’s correlation.

Share and Cite

MDPI and ACS Style

Hsu, C.-N.; Lu, P.-C.; Lo, M.-H.; Lin, I.-C.; Tain, Y.-L. The Association between Nitric Oxide Pathway, Blood Pressure Abnormalities, and Cardiovascular Risk Profile in Pediatric Chronic Kidney Disease. Int. J. Mol. Sci. 2019, 20, 5301. https://doi.org/10.3390/ijms20215301

AMA Style

Hsu C-N, Lu P-C, Lo M-H, Lin I-C, Tain Y-L. The Association between Nitric Oxide Pathway, Blood Pressure Abnormalities, and Cardiovascular Risk Profile in Pediatric Chronic Kidney Disease. International Journal of Molecular Sciences. 2019; 20(21):5301. https://doi.org/10.3390/ijms20215301

Chicago/Turabian Style

Hsu, Chien-Ning, Pei-Chen Lu, Mao-Hung Lo, I-Chun Lin, and You-Lin Tain. 2019. "The Association between Nitric Oxide Pathway, Blood Pressure Abnormalities, and Cardiovascular Risk Profile in Pediatric Chronic Kidney Disease" International Journal of Molecular Sciences 20, no. 21: 5301. https://doi.org/10.3390/ijms20215301

APA Style

Hsu, C.-N., Lu, P.-C., Lo, M.-H., Lin, I.-C., & Tain, Y.-L. (2019). The Association between Nitric Oxide Pathway, Blood Pressure Abnormalities, and Cardiovascular Risk Profile in Pediatric Chronic Kidney Disease. International Journal of Molecular Sciences, 20(21), 5301. https://doi.org/10.3390/ijms20215301

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop