Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae
Abstract
:1. Introduction
2. Results
2.1. PCR-Based Synthesis of Conjugative Plasmid pTA-Mob 2.0
2.2. Development and Optimization of Conjugation within Solid Media
2.3. Conjugation of IncP-Based Plasmids of Increasing Size
3. Discussion
4. Materials and Methods
4.1. Strains and Growth Conditions
4.2. Construction of pTA-Mob 2.0 Plasmid
4.3. Conjugation within Solid Media
4.3.1. Preparation of E. coli
4.3.2. Preparation of S. cerevisiae
4.3.3. Conjugation within Solid Media Procedure
4.3.4. Confirmation of Successfully Transferred Plasmids
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Soltysiak, M.P.M.; Meaney, R.S.; Hamadache, S.; Janakirama, P.; Edgell, D.R.; Karas, B.J. Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae. Int. J. Mol. Sci. 2019, 20, 5212. https://doi.org/10.3390/ijms20205212
Soltysiak MPM, Meaney RS, Hamadache S, Janakirama P, Edgell DR, Karas BJ. Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae. International Journal of Molecular Sciences. 2019; 20(20):5212. https://doi.org/10.3390/ijms20205212
Chicago/Turabian StyleSoltysiak, Maximillian P. M., Rebecca S. Meaney, Samir Hamadache, Preetam Janakirama, David R. Edgell, and Bogumil J. Karas. 2019. "Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae" International Journal of Molecular Sciences 20, no. 20: 5212. https://doi.org/10.3390/ijms20205212