Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots
Abstract
:1. Introduction
2. Results
2.1. HL-Induced Transcriptional Changes in A. thaliana Roots
2.2. HL-Triggered Systemic Changes in the Expression of miRNAs
2.3. Stress Signal is Induced in Rosettes
2.4. Prediction of Potential Targets
3. Discussion
4. Material and Methods
4.1. Plant Material and Growing Conditions
4.2. High-Light Treatment
4.3. Root Separation
4.4. RNA Isolation
4.5. Preparation of Libraries and Micro-Transcriptomic Analysis
4.6. Expression of Marker Genes Determined by RT-qPCR
4.6.1. cDNA Synthesis
4.6.2. Quantitative PCR
4.7. Detection of Mature miRNAs Using Two-Tailed qPCR
4.7.1. cDNA Synthesis
4.7.2. Quantitative PCR
4.8. Detection of Mature miRNA Using Mir-X miRNA
4.8.1. cDNA Synthesis
4.8.2. Quantitative PCR
4.9. Target Transcript Prediction
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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miRNA | Target ID | Target Name | Target Function | References |
---|---|---|---|---|
ath-miR160b | AT2G28350.1 | ARF10 | Response to auxin signaling | [49,50] |
AT4G30080.1 | ARF16 | |||
AT1G77850.1 | ARF17 | |||
ath-miR169f-3p | AT3G59770.1 | SAC9 | Probable phosphoinositide phosphatase | [51] |
ath-miR394a | AT5G36170.2 | HCF109 | Proper translation, stability and processing of polycistronic transcripts in chloroplasts | [52] |
ath-miR8175 | AT2G36250.2 | FtsZ2-1 | Required for plastid division | [53] |
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Anna, B.-B.; Grzegorz, B.; Marek, K.; Piotr, G.; Marcin, F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int. J. Mol. Sci. 2019, 20, 5131. https://doi.org/10.3390/ijms20205131
Anna B-B, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. International Journal of Molecular Sciences. 2019; 20(20):5131. https://doi.org/10.3390/ijms20205131
Chicago/Turabian StyleAnna, Barczak-Brzyżek, Brzyżek Grzegorz, Koter Marek, Gawroński Piotr, and Filipecki Marcin. 2019. "Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots" International Journal of Molecular Sciences 20, no. 20: 5131. https://doi.org/10.3390/ijms20205131