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Article

Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing

1
Dr. Rolf M. Schwiete Research Unit for Osteoarthritis, Orthopaedic University Hospital Friedrichsheim gGmbH, 60528 Frankfurt/Main, Germany
2
Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany
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Institute for Dental Research and Oral Musculoskeletal Biology, Medical Faculty, University of Cologne, 50931 Cologne, Germany
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Center for Molecular Medicine, University of Cologne, 50931 Cologne, Germany
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Department of Dermatology, University of Cologne, 50931 Cologne, Germany
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Department of Pediatrics and Adolescent Medicine, Experimental Neonatology, Medical Faculty, University of Cologne, 50931 Cologne, Germany
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Cologne Center for Musculoskeletal Biomechanics, University of Cologne, 50931 Cologne, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5086; https://doi.org/10.3390/ijms20205086
Received: 23 September 2019 / Revised: 3 October 2019 / Accepted: 11 October 2019 / Published: 14 October 2019
(This article belongs to the Special Issue Extracellular Matrix in Development and Disease 2.0)
The extracellular matrix (ECM) provides structural support for tissue architecture and is a major effector of cell behavior during skin repair and inflammation. Macrophages are involved in all stages of skin repair but only limited knowledge exists about macrophage-specific expression and regulation of ECM components. In this study, we used transcriptome profiling and bioinformatic analysis to define the unique expression of ECM-associated genes in cultured macrophages. Characterization of the matrisome revealed that most genes were constitutively expressed and that several genes were uniquely regulated upon interferon gamma (IFNγ) and dexamethasone stimulation. Among those core matrisome and matrisome-associated components transforming growth factor beta (TGFβ)-induced, matrix metalloproteinase 9 (MMP9), elastin microfibril interfacer (EMILIN)-1, netrin-1 and gliomedin were also present within the wound bed at time points that are characterized by profound macrophage infiltration. Hence, macrophages are a source of ECM components in vitro as well as during skin wound healing, and identification of these matrisome components is a first step to understand the role and therapeutic value of ECM components in macrophages and during wound healing. View Full-Text
Keywords: extracellular matrix; matrisome; pro- and anti-inflammatory macrophage; gene expression profiling; skin wound healing extracellular matrix; matrisome; pro- and anti-inflammatory macrophage; gene expression profiling; skin wound healing
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MDPI and ACS Style

Etich, J.; Koch, M.; Wagener, R.; Zaucke, F.; Fabri, M.; Brachvogel, B. Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing. Int. J. Mol. Sci. 2019, 20, 5086. https://doi.org/10.3390/ijms20205086

AMA Style

Etich J, Koch M, Wagener R, Zaucke F, Fabri M, Brachvogel B. Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing. International Journal of Molecular Sciences. 2019; 20(20):5086. https://doi.org/10.3390/ijms20205086

Chicago/Turabian Style

Etich, Julia, Manuel Koch, Raimund Wagener, Frank Zaucke, Mario Fabri, and Bent Brachvogel. 2019. "Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing" International Journal of Molecular Sciences 20, no. 20: 5086. https://doi.org/10.3390/ijms20205086

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