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Open AccessArticle

Peach Kernel Oil Downregulates Expression of Tissue Factor and Reduces Atherosclerosis in ApoE knockout Mice

by Erwei Hao 1,2,†, Guofeng Pang 1,2,†, Zhengcai Du 1,2,†, Yu-Heng Lai 3, Jung-Ren Chen 4, Jinling Xie 1,2, Kai Zhou 1,2, Xiaotao Hou 1,2,*, Chung-Der Hsiao 5,* and Jiagang Deng 1,2,*
1
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China
2
Guangxi Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China
3
Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
4
Department of Biological Science & Technology College of Medicine, I-Shou University, Kaohsiung 82445, Taiwan
5
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(2), 405; https://doi.org/10.3390/ijms20020405
Received: 19 November 2018 / Revised: 15 January 2019 / Accepted: 16 January 2019 / Published: 18 January 2019
Atherosclerosis is the pathological process in arteries due to the plaque formation that is responsible for several diseases like heart disease, stroke and peripheral arterial disease. In this study, we performed in vitro and in vivo assays to evaluate the potential anti-atherosclerosis activity of peach kernel oil. For the in vitro assay, we incubated human umbilical vein endothelial cells (HUVEC) with tumor necrosis factor-α (TNF-α) to induce tissue factors (TF, an essential mediator of hemostasis and trigger of thrombosis) elevation. We found that TNF-α-induced TF elevation was suppressed by peach kernel oil in a dose-dependent manner at both mRNA and protein levels. Peach kernel oil can significantly improve HUVEC viability, protect the endothelial cells, which achieved the goal of prevention of thrombotic diseases. For the in vivo assay, we investigated the effect and mechanism of peach kernel oil on preventing atherosclerotic lesion formation in ApoE knockout mice. Results show that peach kernel oil could reduce total cholesterol, triglyceride, low-density lipoprotein cholesterol levels, elevate the high-density lipoprotein cholesterol level in serum, and reduce the area of the aortic atherosclerotic lesions in high-fat diet fed ApoE knockout mice. Moreover, peach kernel oil treatment can significantly down regulate the expression of TF protein to inhibit the formation of atherosclerotic plaque. In conclusion, peach kernel oil may be a potential health food to prevent atherosclerosis in cardiovascular diseases. View Full-Text
Keywords: peach kernel oil; tissue factor; atherosclerosis; tumor necrosis factor-α; human umbilical vein endothelial cells peach kernel oil; tissue factor; atherosclerosis; tumor necrosis factor-α; human umbilical vein endothelial cells
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Hao, E.; Pang, G.; Du, Z.; Lai, Y.-H.; Chen, J.-R.; Xie, J.; Zhou, K.; Hou, X.; Hsiao, C.-D.; Deng, J. Peach Kernel Oil Downregulates Expression of Tissue Factor and Reduces Atherosclerosis in ApoE knockout Mice. Int. J. Mol. Sci. 2019, 20, 405.

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