Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor
Abstract
:1. Introduction
2. Results and Discussion
2.1. Highly Efficient Production of Base Edited and Transgene-Free Tomatoes
2.2. Production of Transgene-Free Base Edited Plants in the Tetraploid Potato
3. Materials and Methods
3.1. Vector Cloning
3.2. Agrobacterium-Mediated Transformation
3.3. Genotyping Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ALS | Acetolactate synthase |
CBE | Cytidine base editors |
DSBs | DNA double-strand breaks |
HR | Homologous recombination |
HRM | High resolution melting |
NHEJ | Non-homologous end-joining |
PAM | Protospacer adjacent motif |
PCR | Polymerase chain reaction |
RNP | Ribonucleoprotein |
T-DNA | Transfer DNA |
Target-AID | Target-activation-induced cytidine deaminase |
UGI | Uracil DNA glycosylase inhibitor protein |
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Veillet, F.; Perrot, L.; Chauvin, L.; Kermarrec, M.-P.; Guyon-Debast, A.; Chauvin, J.-E.; Nogué, F.; Mazier, M. Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor. Int. J. Mol. Sci. 2019, 20, 402. https://doi.org/10.3390/ijms20020402
Veillet F, Perrot L, Chauvin L, Kermarrec M-P, Guyon-Debast A, Chauvin J-E, Nogué F, Mazier M. Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor. International Journal of Molecular Sciences. 2019; 20(2):402. https://doi.org/10.3390/ijms20020402
Chicago/Turabian StyleVeillet, Florian, Laura Perrot, Laura Chauvin, Marie-Paule Kermarrec, Anouchka Guyon-Debast, Jean-Eric Chauvin, Fabien Nogué, and Marianne Mazier. 2019. "Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor" International Journal of Molecular Sciences 20, no. 2: 402. https://doi.org/10.3390/ijms20020402