Next Article in Journal
Analytical Methods for Detection of Plant Metabolomes Changes in Response to Biotic and Abiotic Stresses
Next Article in Special Issue
Quo Vadis Biomolecular NMR Spectroscopy?
Previous Article in Journal
Semaphorin Signaling in Cancer-Associated Inflammation
Previous Article in Special Issue
In Situ Monitoring of Bacteria under Antimicrobial Stress Using 31P Solid-State NMR
Article Menu
Issue 2 (January-2) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2019, 20(2), 378; https://doi.org/10.3390/ijms20020378

In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation

1,2,3,*
,
1 and 2,4,*
1
Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK
2
Institute of Physics, Biophysics, Martin–Luther–University Halle–Wittenberg, 06120 Halle, Germany
3
Department of Diabetes, Faculty of Lifesciences and Medicine, King’s College London, Great Maze Pond, London SE1 1UL, UK
4
Centre for Structure und Dynamics of Proteins (MZP), Martin–Luther–University Halle–Wittenberg, 06120 Halle, Germany
*
Authors to whom correspondence should be addressed.
Received: 30 November 2018 / Revised: 11 January 2019 / Accepted: 13 January 2019 / Published: 17 January 2019
(This article belongs to the Special Issue In-Cell NMR Spectroscopy: Biomolecular Structure and Function)
Full-Text   |   PDF [2109 KB, uploaded 17 January 2019]   |  

Abstract

Nuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need to be applied when observing cellular processes under physiological conditions, due to the low sensitivity inherent to the technique. In this review, a brief introduction to in-cell NMR, protein–small molecule interactions, posttranslational phosphorylation, and hyperpolarization NMR methods, used for the study of metabolites in cellulo, are presented. Recent examples of method development in all three fields are conceptually highlighted, and an outlook into future perspectives of this emerging area of NMR research is given. View Full-Text
Keywords: protein NMR; in-cell NMR; in-situ NMR; DNP; review protein NMR; in-cell NMR; in-situ NMR; DNP; review
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Kumar, A.; Kuhn, L.T.; Balbach, J. In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation. Int. J. Mol. Sci. 2019, 20, 378.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top