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Int. J. Mol. Sci. 2019, 20(2), 281; https://doi.org/10.3390/ijms20020281

Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors

1
Department of Analytical Chemistry (C1), Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Cracow, Poland
2
Department of Biophysics, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
3
Centre of Applied Science for Health, Institute of Technology Tallaght, Dublin 24, Ireland
*
Author to whom correspondence should be addressed.
Received: 12 December 2018 / Revised: 3 January 2019 / Accepted: 9 January 2019 / Published: 11 January 2019
(This article belongs to the Section Molecular Biophysics)
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Abstract

A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro. View Full-Text
Keywords: coumarin; hypochlorite sensing; fluorescence quenching coumarin; hypochlorite sensing; fluorescence quenching
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Starzak, K.; Matwijczuk, A.; Creaven, B.; Matwijczuk, A.; Wybraniec, S.; Karcz, D. Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors. Int. J. Mol. Sci. 2019, 20, 281.

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