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Article

Sabinene Prevents Skeletal Muscle Atrophy by Inhibiting the MAPK–MuRF-1 Pathway in Rats

1
Department of Physiology, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
2
Department of Cosmetic Science, College of Life and Health Sciences, Hoseo University, 20 Hoseo-ro79beon-gil, Hoseo-ro, Baebang-eup, Asan 31499, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(19), 4955; https://doi.org/10.3390/ijms20194955
Received: 23 September 2019 / Revised: 28 September 2019 / Accepted: 3 October 2019 / Published: 8 October 2019
(This article belongs to the Section Molecular Biology)
Chrysanthemum boreale Makino essential oil (CBMEO) has diverse biological activities including a skin regenerating effect. However, its role in muscle atrophy remains unknown. This study explored the effects of CBMEO and its active ingredients on skeletal muscle atrophy using in vitro and in vivo models of muscle atrophy. CBMEO reversed the size decrease of L6 myoblasts under starvation. Among the eight monoterpene compounds of CBMEO without cytotoxicity for L6 cells, sabinene induced predominant recovery of reductions of myotube diameters under starvation. Sabinene diminished the elevated E3 ubiquitin ligase muscle ring-finger protein-1 (MuRF-1) expression and p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylations in starved myotubes. Moreover, sabinene decreased the increased level of reactive oxygen species (ROS) in myotubes under starvation. The ROS inhibitor antagonized expression of MuRF-1 and phosphorylation of MAPKs, which were elevated in starved myotubes. In addition, levels of muscle fiber atrophy and MuRF-1 expression in gastrocnemius from fasted rats were reduced after administration of sabinene. These findings demonstrate that sabinene, a bioactive component from CBMEO, may attenuate skeletal muscle atrophy by regulating the activation mechanism of ROS-mediated MAPK/MuRF-1 pathways in starved myotubes, probably leading to the reverse of reduced muscle fiber size in fasted rats. View Full-Text
Keywords: Chrysanthemum boreale Makino essential oil; sabinene; skeletal muscle atrophy; L6 cells; MAPK/MuRF-1 Chrysanthemum boreale Makino essential oil; sabinene; skeletal muscle atrophy; L6 cells; MAPK/MuRF-1
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MDPI and ACS Style

Ryu, Y.; Lee, D.; Jung, S.H.; Lee, K.-J.; Jin, H.; Kim, S.J.; Lee, H.M.; Kim, B.; Won, K.-J. Sabinene Prevents Skeletal Muscle Atrophy by Inhibiting the MAPK–MuRF-1 Pathway in Rats. Int. J. Mol. Sci. 2019, 20, 4955. https://doi.org/10.3390/ijms20194955

AMA Style

Ryu Y, Lee D, Jung SH, Lee K-J, Jin H, Kim SJ, Lee HM, Kim B, Won K-J. Sabinene Prevents Skeletal Muscle Atrophy by Inhibiting the MAPK–MuRF-1 Pathway in Rats. International Journal of Molecular Sciences. 2019; 20(19):4955. https://doi.org/10.3390/ijms20194955

Chicago/Turabian Style

Ryu, Yunkyoung, Donghyen Lee, Seung H. Jung, Kyung-Jin Lee, Hengzhe Jin, Su J. Kim, Hwan M. Lee, Bokyung Kim, and Kyung-Jong Won. 2019. "Sabinene Prevents Skeletal Muscle Atrophy by Inhibiting the MAPK–MuRF-1 Pathway in Rats" International Journal of Molecular Sciences 20, no. 19: 4955. https://doi.org/10.3390/ijms20194955

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