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Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1)

1
Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
2
Malopolska Centre of Biotechnology, Jagiellonian University, ul. Gronostajowa 7a, 30-387 Krakow, Poland
3
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387 Krakow, Poland
4
Center for Integrated Protein Science Munich at Chair of Biomolecular NMR, Department Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(19), 4821; https://doi.org/10.3390/ijms20194821
Received: 4 September 2019 / Revised: 23 September 2019 / Accepted: 25 September 2019 / Published: 28 September 2019
(This article belongs to the Section Molecular Biology)
Glucose phosphorylating enzymes are crucial in the regulation of basic cellular processes, including metabolism and gene expression. Glucokinases and hexokinases provide a pool of phosphorylated glucose in an adenosine diphosphate (ADP)- and ATP-dependent manner to shape the cell metabolism. The glucose processing enzymes from Kluyveromyces lactis are poorly characterized despite the emerging contribution of this yeast strain to industrial and laboratory scale biotechnology. The first reports on K. lactis glucokinase (KlGlk1) positioned the enzyme as an essential component required for glucose signaling. Nevertheless, no biochemical and structural information was available until now. Here, we present the first crystal structure of KlGlk1 together with biochemical characterization, including substrate specificity and enzyme kinetics. Additionally, comparative analysis of the presented structure and the prior structures of lactis hexokinase (KlHxk1) demonstrates the potential transitions between open and closed enzyme conformations upon ligand binding. View Full-Text
Keywords: Kluyveromyces lactis; glucokinase; sugar metabolism Kluyveromyces lactis; glucokinase; sugar metabolism
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Zak, K.M.; Kalińska, M.; Wątor, E.; Kuśka, K.; Krutyhołowa, R.; Dubin, G.; Popowicz, G.M.; Grudnik, P. Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1). Int. J. Mol. Sci. 2019, 20, 4821.

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