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Open AccessArticle

Enhancing the Thermo-Stability and Anti-Biofilm Activity of Alginate Lyase by Immobilization on Low Molecular Weight Chitosan Nanoparticles

1
Department of Pharmacology, College of Basic Medicine, Qingdao University, Qingdao 266071, China
2
Molecular Cancer Biology Laboratory, Cellular Heterogeneity Research Center, Department of Biosystem, Sookmyung Women’s University, Hyochangwon gil-52, Yongsan-Gu, Seoul 140-742, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(18), 4565; https://doi.org/10.3390/ijms20184565
Received: 15 August 2019 / Revised: 10 September 2019 / Accepted: 11 September 2019 / Published: 14 September 2019
(This article belongs to the Special Issue Chitosan-Based Nanoparticles)
Bacterial biofilm causes severe antibiotic resistance. An extracellular polymeric substance (EPS) is the main component in the bacterial biofilm. Alginate is a key EPS component in the biofilm of Pseudomonas aeruginosa and responsible for surface adhesion and stabilization of biofilm. Alginate lyase has emerged as an efficient therapeutic strategy targeting to degrade the alginate in the biofilm of P. aeruginosa. However, the application of this enzyme is limited by its poor stability. In this study, chitosan nanoparticles (CS-NPs) were synthesized using low molecular weight chitosan and alginate lyase Aly08 was immobilized on low molecular weight chitosan nanoparticles (AL-LMW-CS-NPs). As a result, the immobilization significantly enhanced the thermal stability and reusability of Aly08. In addition, compared with free Aly08, the immobilized AL-LMW-CS-NPs exhibited higher efficiency in inhibiting biofilm formation and interrupting the established mature biofilm of P. aeruginosa, which could reduce its biomass and thickness confirmed by confocal microscopy. Moreover, the biofilm disruption greatly increased the antibiotic sensitivity of P. aeruginosa. This research will contribute to the further development of alginate lyase as an anti-biofilm agent. View Full-Text
Keywords: immobilization; chitosan nanoparticles; alginate lyase; anti-biofilm activity; antibiotics susceptibility immobilization; chitosan nanoparticles; alginate lyase; anti-biofilm activity; antibiotics susceptibility
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Li, S.; Wang, Y.; Li, X.; Lee, B.S.; Jung, S.; Lee, M.-S. Enhancing the Thermo-Stability and Anti-Biofilm Activity of Alginate Lyase by Immobilization on Low Molecular Weight Chitosan Nanoparticles. Int. J. Mol. Sci. 2019, 20, 4565.

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