Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals
Abstract
:1. Introduction
2. Results
2.1. Mapping Chicken TNF onto Chicken GGA16 Using Radiation-Hybrid (RH) Panel
2.2. Mapping the Chicken TNF and its Syntenic Genes Using Fluorescent in Situ Hybridization with Tyramide Signal Amplification (FISH-TSA)
2.3. Expression Profiling of mRNAs of TNF, Leptin and Their Cognate Receptors in Immune Cells
2.4. Expression Profiling of Leptin, TNF and Their Cognate Receptors in Variety of Chicken Tissues
2.5. Immunohistochemistry Analysis of Leptin in Chicken Duodenum
3. Discussion
4. Materials and Methods
4.1. RH-Mapping
4.2. FISH-TSA Analysis
4.2.1. Chromosome Preparation and Cell Culture
4.2.2. cDNA Probe Preparation and Labelling
4.2.3. FISH-TSA
4.2.4. Microscopy and Processing of FISH-TSA Images
4.3. Bioinformatic Analysis
4.4. Animals and Tissue Sampling
4.5. Antibodies and Western Analysis
4.6. IHC Analysis
4.7. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Position RH-Mapping (CentiRay) | Linkage by FISH | Position GGA16 | Position HSA6 | |
---|---|---|---|---|---|
TRIM 7.2 | Tripartite motif containing 7 | 200–240 | CSNK2B, TNF | 2,482,253..2,492,415 | |
BRD2 | Bromodomain containing 2 | 294.6 | ABHD16A | 2,574,363..2,583,216 | 32968594..32981505 |
TAP2 | Transporter 2, ATP-binding cassette | 220–300 | 2,601,162..2,604,724 | 32821833..32838823 | |
C4A | Complement 4 | 2,610,038..2,624,392 | 31982057..32002680 | ||
TNF | Tumor Necrosis Factor | 189.1 | Unknown | 31575565..31578336 | |
CSNK2B | Casein kinase II subunit beta | 197.3 | Unknown | 31665880..31670070 | |
ABHD16A | Abhydrolase domain containing 16A | 256 | Unknown | 31686949..31703360 |
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Seroussi, E.; Knytl, M.; Pitel, F.; Elleder, D.; Krylov, V.; Leroux, S.; Morisson, M.; Yosefi, S.; Miyara, S.; Ganesan, S.; et al. Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals. Int. J. Mol. Sci. 2019, 20, 4489. https://doi.org/10.3390/ijms20184489
Seroussi E, Knytl M, Pitel F, Elleder D, Krylov V, Leroux S, Morisson M, Yosefi S, Miyara S, Ganesan S, et al. Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals. International Journal of Molecular Sciences. 2019; 20(18):4489. https://doi.org/10.3390/ijms20184489
Chicago/Turabian StyleSeroussi, Eyal, Martin Knytl, Frédérique Pitel, Daniel Elleder, Vladimir Krylov, Sophie Leroux, Mireille Morisson, Sara Yosefi, Shoval Miyara, Saibaba Ganesan, and et al. 2019. "Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals" International Journal of Molecular Sciences 20, no. 18: 4489. https://doi.org/10.3390/ijms20184489