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Article
Peer-Review Record

Genetic Dissection of Seed Storability and Validation of Candidate Gene Associated with Antioxidant Capability in Rice (Oryza sativa L.)

Int. J. Mol. Sci. 2019, 20(18), 4442; https://doi.org/10.3390/ijms20184442
by Zhiyang Yuan, Kai Fan, Laifu Xia, Xiali Ding, Li Tian, Wenqiang Sun, Hanzi He and Sibin Yu *
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2019, 20(18), 4442; https://doi.org/10.3390/ijms20184442
Submission received: 19 August 2019 / Revised: 6 September 2019 / Accepted: 8 September 2019 / Published: 9 September 2019
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)

Round 1

Reviewer 1 Report

The work from Yuan et al. is investigating the genetic determinants controlling seed storability in rice. Rice seed deterioration results in reduction in seed germination rate, decline in nutritional values and loss of seed viability.  The present study genotyped 334 lines from a backcross recombinant inbred line population and identified several QTLs associated with longer seed storability and germination tolerance to hydrogen peroxide. In addition, the authors found one gene called OsFAH2 and present on one QTL region. Over-expressing lines for OsFAH2 showed increase seed storability, which strongly supports that OsFAH2 is likely the gene responsible for the phenotype of QTL qSS3.1.

 

Overall, the experiments are well documented, and the data are supporting the conclusions. Moreover, the discovery of one gene involved in seed storability in rice has great potential for industrial use.

Author Response

Many thanks for your positive comments.

Reviewer 2 Report

Look at the attached file!

Comments for author File: Comments.pdf

Author Response

Thank you very much for your helpful comments and suggestions. We have revised the manuscript accordingly. Please seed the attachment with the point to point response to the comments.  

Author Response File: Author Response.pdf

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