Genetic Dissection of Seed Storability and Validation of Candidate Gene Associated with Antioxidant Capability in Rice (Oryza sativa L.)
Round 1
Reviewer 1 Report
The work from Yuan et al. is investigating the genetic determinants controlling seed storability in rice. Rice seed deterioration results in reduction in seed germination rate, decline in nutritional values and loss of seed viability. The present study genotyped 334 lines from a backcross recombinant inbred line population and identified several QTLs associated with longer seed storability and germination tolerance to hydrogen peroxide. In addition, the authors found one gene called OsFAH2 and present on one QTL region. Over-expressing lines for OsFAH2 showed increase seed storability, which strongly supports that OsFAH2 is likely the gene responsible for the phenotype of QTL qSS3.1.
Overall, the experiments are well documented, and the data are supporting the conclusions. Moreover, the discovery of one gene involved in seed storability in rice has great potential for industrial use.
Author Response
Many thanks for your positive comments.
Reviewer 2 Report
Look at the attached file!
Comments for author File: Comments.pdf
Author Response
Thank you very much for your helpful comments and suggestions. We have revised the manuscript accordingly. Please seed the attachment with the point to point response to the comments.
Author Response File: Author Response.pdf