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Open AccessArticle

Genetic Dissection of Seed Storability and Validation of Candidate Gene Associated with Antioxidant Capability in Rice (Oryza sativa L.)

National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430000, Hubei, China
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(18), 4442; https://doi.org/10.3390/ijms20184442
Received: 19 August 2019 / Revised: 6 September 2019 / Accepted: 8 September 2019 / Published: 9 September 2019
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
Seed storability, defined as the ability to remain alive during storage, is an important agronomic and physiological characteristic, but the underlying genetic mechanism remains largely unclear. Here, we report quantitative trait loci (QTLs) analyses for seed storability using a high-density single nucleotide polymorphism linkage map in the backcross recombinant inbred lines that was derived from a cross of a japonica cultivar, Nipponbare, and an indica cultivar, 9311. Seven putative QTLs were identified for seed storability under natural storage, each explaining 3.6–9.0% of the phenotypic variation in this population. Among these QTLs, qSS1 with the 9311 alleles promoting seed storability was further validated in near-isogenic line and its derived-F2 population. The other locus (qSS3.1) for seed storability colocalized with a locus for germination ability under hydrogen peroxide, which is recognized as an oxidant molecule that causes lipid damage. Transgenic experiments validated that a candidate gene (OsFAH2) resides the qSS3.1 region controlling seed storability and antioxidant capability. Overexpression of OsFAH2 that encodes a fatty acid hydroxylase reduced lipid preoxidation and increased seed storability. These findings provide new insights into the genetic and physiological bases of seed storability and will be useful for the improvement of seed storability in rice. View Full-Text
Keywords: seed viability; quantitative trait locus; fatty acid hydroxylase; oxidative stress; genotyping by sequencing; rice seed viability; quantitative trait locus; fatty acid hydroxylase; oxidative stress; genotyping by sequencing; rice
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Yuan, Z.; Fan, K.; Xia, L.; Ding, X.; Tian, L.; Sun, W.; He, H.; Yu, S. Genetic Dissection of Seed Storability and Validation of Candidate Gene Associated with Antioxidant Capability in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2019, 20, 4442.

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