TRPM6 N-Terminal CaM- and S100A1-Binding Domains
Abstract
:1. Introduction
2. Results
2.1. The Identification of TRPM6np Binding Domains for CaM and S100A1
2.2. CaM- and S100A1-Binding Domains of the TRPM6 N-Termini Overlap
2.3. Characterisation of the TRPM6np/CaM Complex
2.4. Characterisation of the TRPM6np/S100A1 Complex
2.5. TRPM6np Molecular Modelling and CaM and S100A1 Docking
3. Discussion
4. Methods
4.1. TRPM6-Binding Domain Design and Synthesis
4.2. Expression and Purification of CaM and S100A1
4.3. Steady-State Fluorescence Anisotropy Measurements
4.4. Lifetime Experiments
4.5. Model Building
4.6. Ligand Docking
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ATP | adenosine trisphosphate |
CaM | calmodulin |
CBPs | calcium-binding proteins |
Cryo-EM | Cryogenic Electron Microscopy |
IDPs | intrinsically disordered proteins |
PIP2 | phosphatidylinositol 4, 5- bisphosphate |
RyR | rhyanodine receptor |
S100A1 | S100 calcium-binding protein A1 |
TRP | transient receptor potential channel |
TRPM | transient receptor potential channel melastatin |
TRPM6np | transient receptor potential channel melastatin 6 N-terminal peptide |
TRPs | transient receptor potential channels |
TRPV | transient receptor potential channels |
WT | wild type |
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Zouharova, M.; Herman, P.; Hofbauerová, K.; Vondrasek, J.; Bousova, K. TRPM6 N-Terminal CaM- and S100A1-Binding Domains. Int. J. Mol. Sci. 2019, 20, 4430. https://doi.org/10.3390/ijms20184430
Zouharova M, Herman P, Hofbauerová K, Vondrasek J, Bousova K. TRPM6 N-Terminal CaM- and S100A1-Binding Domains. International Journal of Molecular Sciences. 2019; 20(18):4430. https://doi.org/10.3390/ijms20184430
Chicago/Turabian StyleZouharova, Monika, Petr Herman, Kateřina Hofbauerová, Jiri Vondrasek, and Kristyna Bousova. 2019. "TRPM6 N-Terminal CaM- and S100A1-Binding Domains" International Journal of Molecular Sciences 20, no. 18: 4430. https://doi.org/10.3390/ijms20184430