Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

Ziemens, Annalisa; Sonntag, Svenja R.; Wulfmeyer, Vera C.; Edemir, Bayram; Bleich, Markus; Himmerkus, Nina

doi:10.3390/ijms20184401

Open AccessArticle

Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

by

Annalisa Ziemens

¹,

Svenja R. Sonntag

^1,2,

Vera C. Wulfmeyer

^1,3,

Bayram Edemir

⁴,

Markus Bleich

¹ and

Nina Himmerkus

^1,*

¹

Institute of Physiology, Christian-Albrecht-University Kiel, Hermann-Rodewald-Str. 5, 24118 Kiel, Germany

²

Department of Ophthalmology, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

³

Department of Nephrology and Hypertension, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

⁴

Department of Internal Medicine IV, Hematology and Oncology, University Hospital Halle, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(18), 4401; https://doi.org/10.3390/ijms20184401

Submission received: 26 July 2019 / Revised: 29 August 2019 / Accepted: 4 September 2019 / Published: 7 September 2019

(This article belongs to the Special Issue The Tight Junction and Its Proteins: More Than Just a Barrier)

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Abstract

The inner medullary collecting duct (IMCD) is subject to severe changes in ambient osmolality and must either allow water transport or be able to seal the lumen against a very high osmotic pressure. We postulate that the tight junction protein claudin-19 is expressed in IMCD and that it takes part in epithelial adaptation to changing osmolality at different functional states. Presence of claudin-19 in rat IMCD was investigated by Western blotting and immunofluorescence. Primary cell culture of rat IMCD cells on permeable filter supports was performed under different osmotic culture conditions and after stimulation by antidiuretic hormone (AVP). Electrogenic transepithelial transport properties were measured in Ussing chambers. IMCD cells cultivated at 300 mosm/kg showed high transepithelial resistance, a cation selective paracellular pathway and claudin-19 was mainly located in the tight junction. Treatment by AVP increased cation selectivity but did not alter transepithelial resistance or claudin-19 subcellular localization. In contrast, IMCD cells cultivated at 900 mosm/kg had low transepithelial resistance, anion selectivity, and claudin-19 was relocated from the tight junctions to intracellular vesicles. The data shows osmolality-dependent transformation of IMCD epithelium from tight and sodium-transporting to leaky, with claudin-19 expression in the tight junction associated to tightness and cation selectivity under low osmolality.

Keywords: barrier function; paracellular permeability; antidiuretic hormone

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MDPI and ACS Style

Ziemens, A.; Sonntag, S.R.; Wulfmeyer, V.C.; Edemir, B.; Bleich, M.; Himmerkus, N. Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells. Int. J. Mol. Sci. 2019, 20, 4401. https://doi.org/10.3390/ijms20184401

AMA Style

Ziemens A, Sonntag SR, Wulfmeyer VC, Edemir B, Bleich M, Himmerkus N. Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells. International Journal of Molecular Sciences. 2019; 20(18):4401. https://doi.org/10.3390/ijms20184401

Chicago/Turabian Style

Ziemens, Annalisa, Svenja R. Sonntag, Vera C. Wulfmeyer, Bayram Edemir, Markus Bleich, and Nina Himmerkus. 2019. "Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells" International Journal of Molecular Sciences 20, no. 18: 4401. https://doi.org/10.3390/ijms20184401

APA Style

Ziemens, A., Sonntag, S. R., Wulfmeyer, V. C., Edemir, B., Bleich, M., & Himmerkus, N. (2019). Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells. International Journal of Molecular Sciences, 20(18), 4401. https://doi.org/10.3390/ijms20184401

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Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

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