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Article

Cyclin–CDK Complexes are Key Controllers of Capacitation-Dependent Actin Dynamics in Mammalian Spermatozoa

1
Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy
2
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Via Campo Boario 1, 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(17), 4236; https://doi.org/10.3390/ijms20174236
Received: 12 August 2019 / Revised: 24 August 2019 / Accepted: 26 August 2019 / Published: 29 August 2019
(This article belongs to the Special Issue Cyclin-Dependent Kinases in Health and Diseases)
Mammalian spermatozoa are infertile immediately after ejaculation and need to undergo a functional maturation process to acquire the competence to fertilize the female egg. During this process, called capacitation, the actin cytoskeleton dramatically changes its organization. First, actin fibers polymerize, forming a network over the anterior part of the sperm cells head, and then it rapidly depolymerizes and disappears during the exocytosis of the acrosome content (the acrosome reaction (AR)). Here, we developed a computational model representing the actin dynamics (AD) process on mature spermatozoa. In particular, we represented all the molecular events known to be involved in AD as a network of nodes linked by edges (the interactions). After the network enrichment, using an online resource (STRING), we carried out the statistical analysis on its topology, identifying the controllers of the system and validating them in an experiment of targeted versus random attack to the network. Interestingly, among them, we found that cyclin-dependent kinase (cyclin–CDK) complexes are acting as stronger controllers. This finding is of great interest since it suggests the key role that cyclin–CDK complexes could play in controlling AD during sperm capacitation, leading us to propose a new and interesting non-genomic role for these molecules. View Full-Text
Keywords: spermatozoa; cyclin–CDK complexes; actin dynamics; capacitation; acrosome reaction; biological network; computational model spermatozoa; cyclin–CDK complexes; actin dynamics; capacitation; acrosome reaction; biological network; computational model
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MDPI and ACS Style

Bernabò, N.; Ramal-Sanchez, M.; Valbonetti, L.; Machado-Simoes, J.; Ordinelli, A.; Capacchietti, G.; Taraschi, A.; Barboni, B. Cyclin–CDK Complexes are Key Controllers of Capacitation-Dependent Actin Dynamics in Mammalian Spermatozoa. Int. J. Mol. Sci. 2019, 20, 4236. https://doi.org/10.3390/ijms20174236

AMA Style

Bernabò N, Ramal-Sanchez M, Valbonetti L, Machado-Simoes J, Ordinelli A, Capacchietti G, Taraschi A, Barboni B. Cyclin–CDK Complexes are Key Controllers of Capacitation-Dependent Actin Dynamics in Mammalian Spermatozoa. International Journal of Molecular Sciences. 2019; 20(17):4236. https://doi.org/10.3390/ijms20174236

Chicago/Turabian Style

Bernabò, Nicola, Marina Ramal-Sanchez, Luca Valbonetti, Juliana Machado-Simoes, Alessandra Ordinelli, Giulia Capacchietti, Angela Taraschi, and Barbara Barboni. 2019. "Cyclin–CDK Complexes are Key Controllers of Capacitation-Dependent Actin Dynamics in Mammalian Spermatozoa" International Journal of Molecular Sciences 20, no. 17: 4236. https://doi.org/10.3390/ijms20174236

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