Transcriptome Profiling of Placenta through Pregnancy Reveals Dysregulation of Bile Acids Transport and Detoxification Function
Abstract
:1. Introduction
2. Results
2.1. RNA Sequencing and Identification of mRNAs in All Porcine Placentas
2.2. RNA Sequencing and Identification of Long Non-Coding RNAs (lncRNAs) in All Porcine Placentas
2.3. Comparison of Features of mRNAs and Long Non-Coding RNAs (lncRNAs)
2.4. Placentas at Different Time of Pregnancy Have Distinct Gene Expression Profiles and Cluster Separately
2.5. Dysfunction of Bile Acid Transport and Detoxification Are the Central Characteristics with the Advance of Pregnancy
2.6. Prediction of Long Non-Coded RNAs (LncRNAs) Function
3. Discussion
4. Materials and Methods
4.1. Experimental Design and Sampling
4.2. Total Bile Acids
4.3. RNA Sequencing and Data Analysis of the Porcine Placentas
4.4. Real-Time RT-PCR
4.5. Measurement of C5a
4.6. Data Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ICP | Intrahepatic cholestasis of pregnancy |
RNA-Seq | RNA sequencing |
BA | Bile acid |
LncRNA | Long non-coding RNA |
ABCB11/BSEP | Bile salt export pump |
OATP1A2/SLCO1A2 | Organic anion-transporting polypeptide 1A2 |
TPMa | Apical plasma membrane of the trophoblast |
TPMb | Basal plasma membrane of the trophoblast |
NBC1/SLC4A4 | Na+-HCO3− cotransporter |
SULT2A1 | Hydroxysteroid sulfotransferases |
CA2 | Carbonate anhydrase 2 |
FXR/NR1H4 | Farnesoid X receptor |
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Wang, P.; Song, Y.; Zhong, H.; Lin, S.; Zhang, X.; Li, J.; Che, L.; Feng, B.; Lin, Y.; Xu, S.; et al. Transcriptome Profiling of Placenta through Pregnancy Reveals Dysregulation of Bile Acids Transport and Detoxification Function. Int. J. Mol. Sci. 2019, 20, 4099. https://doi.org/10.3390/ijms20174099
Wang P, Song Y, Zhong H, Lin S, Zhang X, Li J, Che L, Feng B, Lin Y, Xu S, et al. Transcriptome Profiling of Placenta through Pregnancy Reveals Dysregulation of Bile Acids Transport and Detoxification Function. International Journal of Molecular Sciences. 2019; 20(17):4099. https://doi.org/10.3390/ijms20174099
Chicago/Turabian StyleWang, Peng, Yumo Song, Heju Zhong, Sen Lin, Xiaoling Zhang, Jian Li, Lianqiang Che, Bin Feng, Yan Lin, Shengyu Xu, and et al. 2019. "Transcriptome Profiling of Placenta through Pregnancy Reveals Dysregulation of Bile Acids Transport and Detoxification Function" International Journal of Molecular Sciences 20, no. 17: 4099. https://doi.org/10.3390/ijms20174099