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Open AccessArticle

MicroRNA Profiling During Neural Differentiation of Induced Pluripotent Stem Cells

1
Radiobiology Lab, Department of Medical Physics, Greater Poland Cancer, 61-866 Poznań, Poland
2
Department of Pathology, Poznan University of Medical Sciences and Greater Poland Cancer Center, Garbary 15th Street, 61-866 Poznan, Poland
3
Department of Histology and Embryology, Poznan University of Medical Sciences, Swiecickiego 6 Street, 60-781 Poznan, Poland
4
Department of Molecular Biomedicine, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14 Str., 61-704 Poznan, Poland
5
Department of Electroradiology, Poznan University of Medical Sciences, Garbary 15th Street, 61-866 Poznan, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(15), 3651; https://doi.org/10.3390/ijms20153651
Received: 19 June 2019 / Revised: 17 July 2019 / Accepted: 25 July 2019 / Published: 26 July 2019
(This article belongs to the Special Issue MicroRNAs and Stem Cells)
MicroRNAs (miRNA) play an essential role in the regulation of gene expression and influence signaling networks responsible for several cellular processes like differentiation of pluripotent stem cells. Despite several studies on the neurogenesis process, no global analysis of microRNA expression during differentiation of induced pluripotent stem cells (iPSC) to neuronal stem cells (NSC) has been done. Therefore, we compared the profile of microRNA expression in iPSC lines and in NSC lines derived from them, using microarray-based analysis. Two different protocols for NSC formation were used: Direct and two-step via neural rosette formation. We confirmed the new associations of previously described miRNAs in regulation of NSC differentiation from iPSC. We discovered upregulation of miR-10 family, miR-30 family and miR-9 family and downregulation of miR-302 and miR-515 family expression. Moreover, we showed that miR-10 family play a crucial role in the negative regulation of genes expression belonging to signaling pathways involved in neural differentiation: WNT signaling pathway, focal adhesion, and signaling pathways regulating pluripotency of stem cells. View Full-Text
Keywords: human induced pluripotent stem cells; neural differentiation; neural stem cells; microRNA human induced pluripotent stem cells; neural differentiation; neural stem cells; microRNA
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Kulcenty, K.; Wroblewska, J.P.; Rucinski, M.; Kozlowska, E.; Jopek, K.; Suchorska, W.M. MicroRNA Profiling During Neural Differentiation of Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 2019, 20, 3651.

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