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Article

Transcriptome-Based Analysis Reveals a Crucial Role of BxGPCR17454 in Low Temperature Response of Pine Wood Nematode (Bursaphelenchus xylophilus)

by 1, 1, 1, 2,* and 1,*
1
College of Forestry, Northeast Forestry University, Harbin 150040, China
2
College of Management, Harbin University of Commerce, Harbin 150028, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2898; https://doi.org/10.3390/ijms20122898
Received: 9 April 2019 / Revised: 6 June 2019 / Accepted: 11 June 2019 / Published: 14 June 2019
(This article belongs to the Section Biochemistry)
Background: The causal agent of pine wilt disease is the pine wood nematode (PWN) (Bursaphelenchus xylophilus), whose ability to adapt different ecological niches is a crucial determinant of their invasion to colder regions. To discover the molecular mechanism of low temperature response mechanism, we attempted to study the molecular response patterns under low temperature from B. xylophilus with a comprehensive RNA sequencing analysis and validated the differentially expressed genes (DEGs) with quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatic software was utilized to isolate and identify the low-temperature-related BxGPCR genes. Transcript abundance of six low-temperature-related BxGPCR genes and function of one of the BxGPCR genes are studied by qRT-PCR and RNA interference. Results: The results showed that we detected 432 DEGs through RNA sequencing between low-temperature-treated and ambient-temperature-treated groups nematodes. The transcript level of 6 low-temperature-related BxGPCR genes increased at low temperature. And, the survival rates of BxGPCR17454 silenced B. xylophilus revealed a significant decrease at low temperature. Conclusion: in conclusion, this transcriptome-based study revealed a crucial role of BxGPCR17454 in low temperature response process of pine wood nematode. These discoveries would assist the development of management and methods for efficient control of this devastating pine tree pest. View Full-Text
Keywords: Bursaphelenchus xylophilus; pine wilt disease; low temperature; transcriptome; GPCR; RNAi Bursaphelenchus xylophilus; pine wilt disease; low temperature; transcriptome; GPCR; RNAi
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MDPI and ACS Style

Wang, B.; Hao, X.; Xu, J.; Ma, Y.; Ma, L. Transcriptome-Based Analysis Reveals a Crucial Role of BxGPCR17454 in Low Temperature Response of Pine Wood Nematode (Bursaphelenchus xylophilus). Int. J. Mol. Sci. 2019, 20, 2898. https://doi.org/10.3390/ijms20122898

AMA Style

Wang B, Hao X, Xu J, Ma Y, Ma L. Transcriptome-Based Analysis Reveals a Crucial Role of BxGPCR17454 in Low Temperature Response of Pine Wood Nematode (Bursaphelenchus xylophilus). International Journal of Molecular Sciences. 2019; 20(12):2898. https://doi.org/10.3390/ijms20122898

Chicago/Turabian Style

Wang, Bowen, Xin Hao, Jiayao Xu, Yan Ma, and Ling Ma. 2019. "Transcriptome-Based Analysis Reveals a Crucial Role of BxGPCR17454 in Low Temperature Response of Pine Wood Nematode (Bursaphelenchus xylophilus)" International Journal of Molecular Sciences 20, no. 12: 2898. https://doi.org/10.3390/ijms20122898

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