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Transcription Profiles Reveal the Regulatory Synthesis of Phenols during the Development of Lotus Rhizome (Nelumbo nucifera Gaertn)

1
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
2
School Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(11), 2735; https://doi.org/10.3390/ijms20112735
Received: 22 April 2019 / Revised: 23 May 2019 / Accepted: 2 June 2019 / Published: 4 June 2019
(This article belongs to the Section Molecular Plant Sciences)
Lotus (Nelumbo nucifera Gaertn) is a wetland vegetable famous for its nutritional and medicinal value. Phenolic compounds are secondary metabolites that play important roles in the browning of fresh-cut fruits and vegetables, and chemical constituents are extracted from lotus for medicine due to their high antioxidant activity. Studies have explored in depth the changes in phenolic compounds during browning, while little is known about their synthesis during the formation of lotus rhizome. In this study, transcriptomic analyses of six samples were performed during lotus rhizome formation using a high-throughput tag sequencing technique. About 23 million high-quality reads were generated, and 92.14% of the data was mapped to the reference genome. The samples were divided into two stages, and we identified 23,475 genes in total, 689 of which were involved in the biosynthesis of secondary metabolites. A complex genetic crosstalk-regulated network involved in the biosynthesis of phenolic compounds was found during the development of lotus rhizome, and 25 genes in the phenylpropanoid biosynthesis pathway, 18 genes in the pentose phosphate pathway, and 30 genes in the flavonoid biosynthesis pathway were highly expressed. The expression patterns of key enzymes assigned to the synthesis of phenolic compounds were analyzed. Moreover, several differentially expressed genes required for phenolic compound biosynthesis detected by comparative transcriptomic analysis were verified through qRT-PCR. This work lays a foundation for future studies on the molecular mechanisms of phenolic compound biosynthesis during rhizome formation. View Full-Text
Keywords: RNA-seq; lotus rhizome; phenols; qRT-PCR RNA-seq; lotus rhizome; phenols; qRT-PCR
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MDPI and ACS Style

Min, T.; Bao, Y.; Zhou, B.; Yi, Y.; Wang, L.; Hou, W.; Ai, Y.; Wang, H. Transcription Profiles Reveal the Regulatory Synthesis of Phenols during the Development of Lotus Rhizome (Nelumbo nucifera Gaertn). Int. J. Mol. Sci. 2019, 20, 2735. https://doi.org/10.3390/ijms20112735

AMA Style

Min T, Bao Y, Zhou B, Yi Y, Wang L, Hou W, Ai Y, Wang H. Transcription Profiles Reveal the Regulatory Synthesis of Phenols during the Development of Lotus Rhizome (Nelumbo nucifera Gaertn). International Journal of Molecular Sciences. 2019; 20(11):2735. https://doi.org/10.3390/ijms20112735

Chicago/Turabian Style

Min, Ting; Bao, Yinqiu; Zhou, Baixue; Yi, Yang; Wang, Limei; Hou, Wenfu; Ai, Youwei; Wang, Hongxun. 2019. "Transcription Profiles Reveal the Regulatory Synthesis of Phenols during the Development of Lotus Rhizome (Nelumbo nucifera Gaertn)" Int. J. Mol. Sci. 20, no. 11: 2735. https://doi.org/10.3390/ijms20112735

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