Next Article in Journal
Proliferative Pathways of Vascular Smooth Muscle Cells in Response to Intermittent Hypoxia
Next Article in Special Issue
Immortalized Human hTert/KER-CT Keratinocytes a Model System for Research on Desmosomal Adhesion and Pathogenesis of Pemphigus Vulgaris
Previous Article in Journal
Resveratrol Action on Lipid Metabolism in Cancer
Previous Article in Special Issue
Akt Signaling in Macrophage Polarization, Survival, and Atherosclerosis
Article Menu
Issue 11 (June-1) cover image

Export Article

Open AccessArticle

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(11), 2705; https://doi.org/10.3390/ijms20112705
Received: 5 May 2019 / Revised: 26 May 2019 / Accepted: 30 May 2019 / Published: 1 June 2019
(This article belongs to the Collection Feature Papers in Molecular Biology)
  |  
PDF [4296 KB, uploaded 4 June 2019]
  |  

Abstract

Sea urchin (Mesocentrotus nudus) is an economically important mariculture species in China and the gonads are the solely edible parts to human. The molecular mechanisms of gonad development have attracted increasing attention in recent years. Although the nanos2 gene has been identified as a germ cell marker in several invertebrates, little is known about nanos2 in adult sea urchins. Hereinto, we report the characterization of Mnnano2, an M. nudus nanos2 homology gene. Mnnanos2 is a maternal factor and can be detected continuously during embryogenesis and early ontogeny. Real-time quantitative PCR (RT-qPCR) and section in situ hybridization (ISH) analysis revealed a dynamic and sexually dimorphic expression pattern of Mnnano2 in the gonads. Its expression reached the maximal level at Stage 2 along with the gonad development in both ovary and testis. In the ovary, Mnnanos2 is specifically expressed in germ cells. In contrast, Mnnanos2 is expressed in both nutritive phagocytes (NP) cells and male germ cells in testis. Moreover, knocking down of Mnnanos2 by means of RNA interference (RNAi) reduced nanos2 and boule expression but conversely increased the expression of foxl2. Therefore, our data suggest that Mnnanos2 may serve as a female germ cell marker during gametogenesis and provide chances to uncover its function in adult sea urchin. View Full-Text
Keywords: sea urchins; nanos2; germ cell; RNAi sea urchins; nanos2; germ cell; RNAi
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Zhang, J.; Han, X.; Wang, J.; Liu, B.-Z.; Wei, J.-L.; Zhang, W.-J.; Sun, Z.-H.; Chang, Y.-Q. Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus. Int. J. Mol. Sci. 2019, 20, 2705.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top