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Fine Mapping of the Wheat Leaf Rust Resistance Gene Lr42

1
Department of Agronomy, Horticulture & Plant Science, South Dakota State University, Brookings, SD 57006, USA
2
National Engineering Laboratory of Wheat, Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
3
The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
4
Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
5
USDA-ARS, Hard Winter Wheat Genetic Research Unit, Manhattan, KS 66506, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(10), 2445; https://doi.org/10.3390/ijms20102445 (registering DOI)
Received: 22 March 2019 / Revised: 6 May 2019 / Accepted: 14 May 2019 / Published: 17 May 2019
(This article belongs to the Special Issue Molecular Advances in Wheat and Barley)
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Abstract

Leaf rust caused by Puccinia triticina Eriks is one of the most problematic diseases of wheat throughout the world. The gene Lr42 confers effective resistance against leaf rust at both seedling and adult plant stages. Previous studies had reported Lr42 to be both recessive and dominant in hexaploid wheat; however, in diploid Aegilops tauschii (TA2450), we found Lr42 to be dominant by studying segregation in two independent F2 and their F2:3 populations. We further fine-mapped Lr42 in hexaploid wheat using a KS93U50/Morocco F5 recombinant inbred line (RIL) population to a 3.7 cM genetic interval flanked by markers TC387992 and WMC432. The 3.7 cM Lr42 region physically corresponds to a 3.16 Mb genomic region on chromosome 1DS based on the Chinese Spring reference genome (RefSeq v.1.1) and a 3.5 Mb genomic interval on chromosome 1 in the Ae. tauschii reference genome. This region includes nine nucleotide-binding domain leucine-rich repeat (NLR) genes in wheat and seven in Ae. tauschii, respectively, and these are the likely candidates for Lr42. Furthermore, we developed two kompetitive allele-specific polymorphism (KASP) markers (SNP113325 and TC387992) flanking Lr42 to facilitate marker-assisted selection for rust resistance in wheat breeding programs. View Full-Text
Keywords: wheat; Aegilops tauschii; Lr42; disease resistance; molecular mapping; KASP markers; marker-assisted selection wheat; Aegilops tauschii; Lr42; disease resistance; molecular mapping; KASP markers; marker-assisted selection
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Gill, H.S.; Li, C.; Sidhu, J.S.; Liu, W.; Wilson, D.; Bai, G.; Gill, B.S.; Sehgal, S.K. Fine Mapping of the Wheat Leaf Rust Resistance Gene Lr42. Int. J. Mol. Sci. 2019, 20, 2445.

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