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Article

Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus

by
Ning Wang
1,2,†,
Jiafei Zhan
1,†,
Cheng Guo
1,
Chunyan Li
1,
Nengxing Shen
1,
Xiaobin Gu
1,
Yue Xie
1,
Xuerong Peng
3 and
Guangyou Yang
1,*
1
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2
College of Bioengineering, Sichuan University of Science and Engineering, Yibin 644000, China
3
Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(9), 2669; https://doi.org/10.3390/ijms19092669
Submission received: 22 July 2018 / Revised: 22 August 2018 / Accepted: 5 September 2018 / Published: 8 September 2018
(This article belongs to the Section Biochemistry)
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Abstract

Cystic echinococcosis, a parasitic zoonosis that causes significant economic losses and poses a threat to public health, is caused by larvae of the tapeworm Echinococcus granulosus. Infection causes infertile cysts in intermediate hosts that cannot produce protoscoleces (PSCs) or complete the life cycle. Herein, we cloned, expressed, and characterised mitochondrial fission protein 1 (Eg-Fis1) and programmed cell death protein 6 (Eg-PDCD6) from E. granulosus, and explored their functions related to infertile cysts. Eg-Fis1 and Eg-PDCD6 encode putative 157 and 174 residue proteins, respectively, and Western blotting indicated good reactogenicity for both. Eg-Fis1 and Eg-PDCD6 were ubiquitously distributed in all stages of E. granulosus. Furthermore, mRNAs of Eg-Fis1 and Eg-PDCD6 were upregulated following H2O2 treatment which induced apoptosis in PSCs. To investigate the regulation of apoptosis in response to oxidative stress, RNA interference (RNAi) and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assays were performed. The apoptotic rate of the Eg-Fis1 RNAi group was significantly lower than non-interference group, but there was no such difference for Eg-PDCD6. In conclusion, Eg-Fis1 promotes apoptosis induced by oxidative stress, whereas Eg-PDCD6 does not appear to be a key regulator of apoptosis.
Keywords: mitochondrial fission protein 1; programmed cell death protein 6; Echinococcus granulosus; infertile cyst; apoptosis; oxidative stress mitochondrial fission protein 1; programmed cell death protein 6; Echinococcus granulosus; infertile cyst; apoptosis; oxidative stress
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MDPI and ACS Style

Wang, N.; Zhan, J.; Guo, C.; Li, C.; Shen, N.; Gu, X.; Xie, Y.; Peng, X.; Yang, G. Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus. Int. J. Mol. Sci. 2018, 19, 2669. https://doi.org/10.3390/ijms19092669

AMA Style

Wang N, Zhan J, Guo C, Li C, Shen N, Gu X, Xie Y, Peng X, Yang G. Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus. International Journal of Molecular Sciences. 2018; 19(9):2669. https://doi.org/10.3390/ijms19092669

Chicago/Turabian Style

Wang, Ning, Jiafei Zhan, Cheng Guo, Chunyan Li, Nengxing Shen, Xiaobin Gu, Yue Xie, Xuerong Peng, and Guangyou Yang. 2018. "Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus" International Journal of Molecular Sciences 19, no. 9: 2669. https://doi.org/10.3390/ijms19092669

APA Style

Wang, N., Zhan, J., Guo, C., Li, C., Shen, N., Gu, X., Xie, Y., Peng, X., & Yang, G. (2018). Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus. International Journal of Molecular Sciences, 19(9), 2669. https://doi.org/10.3390/ijms19092669

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