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Int. J. Mol. Sci. 2018, 19(9), 2655; https://doi.org/10.3390/ijms19092655

Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions

1
Graduate School of Science and Technology, Niigata University, 2-8050 Ikarashi, Niigata 950-2181, Japan
2
Department of Biochemistry, Niigata University, Niigata 950-218, Japan
3
Instituto de Agrobiotecnología (CSIC, UPNA, Gobierno de Navarra), Mutiloako Etorbidea Zenbaki Gabe, 31192 Mutiloabeti, Nafarroa, Spain
*
Author to whom correspondence should be addressed.
Received: 15 August 2018 / Revised: 30 August 2018 / Accepted: 3 September 2018 / Published: 7 September 2018
(This article belongs to the Special Issue Plant Proteomic Research 2.0)
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Abstract

Rice nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) catalyzes the hydrolytic breakdown of the pyrophosphate and phosphodiester bonds of a number of nucleotides including ADP-glucose and ATP. Under high temperature and elevated CO2 conditions (HT + ECO2), the npp1 knockout rice mutant displayed rapid growth and high starch content phenotypes, indicating that NPP1 exerts a negative effect on starch accumulation and growth. To gain further insight into the mechanisms involved in the NPP1 downregulation induced starch overaccumulation, in this study we conducted photosynthesis, leaf proteomic, and chloroplast phosphoproteomic analyses of wild-type (WT) and npp1 plants cultured under HT + ECO2. Photosynthesis in npp1 leaves was significantly higher than in WT. Additionally, npp1 leaves accumulated higher levels of sucrose than WT. The proteomic analyses revealed upregulation of proteins related to carbohydrate metabolism and the protein synthesis system in npp1 plants. Further, our data indicate the induction of 14-3-3 proteins in npp1 plants. Our finding demonstrates a higher level of protein phosphorylation in npp1 chloroplasts, which may play an important role in carbohydrate accumulation. Together, these results offer novel targets and provide additional insights into carbohydrate metabolism regulation under ambient and adverse conditions. View Full-Text
Keywords: chloroplast; elevated CO2; heat stress; nucleotide pyrophosphatase/phosphodiesterase; (phospho)-proteomics; photosynthesis; protein phosphorylation; 14-3-3 proteins; Oryza sativa L.; starch; sucrose chloroplast; elevated CO2; heat stress; nucleotide pyrophosphatase/phosphodiesterase; (phospho)-proteomics; photosynthesis; protein phosphorylation; 14-3-3 proteins; Oryza sativa L.; starch; sucrose
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Inomata, T.; Baslam, M.; Masui, T.; Koshu, T.; Takamatsu, T.; Kaneko, K.; Pozueta-Romero, J.; Mitsui, T. Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions. Int. J. Mol. Sci. 2018, 19, 2655.

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