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Int. J. Mol. Sci. 2018, 19(9), 2590; https://doi.org/10.3390/ijms19092590

Recombinant Escherichia coli BL21-pET28a-egfp Cultivated with Nanomaterials in a Modified Microchannel for Biofilm Formation

1
School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China
2
Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China
*
Author to whom correspondence should be addressed.
Received: 8 August 2018 / Revised: 26 August 2018 / Accepted: 27 August 2018 / Published: 31 August 2018
(This article belongs to the Special Issue Immobilization of Microorganisms and Enzymes)
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Abstract

The application of whole cells as catalytic biofilms in microchannels has attracted increasing scientific interest. However, the excessive biomass formation and structure of biofilms in a reactor limits their use. A microchannel reactor with surface modification was used to colonize recombinant Escherichia coil BL21-pET28a-egfp rapidly and accelerated growth of biofilms in the microchannel. The segmented flow system of ‘air/culture medium containing nanomaterials’ was firstly used to modulate the biofilms formation of recombinant E. coil; the inhibitory effects of nanomaterials on biofilm formation were investigated. The results indicated that the segmental flow mode has a significant impact on the structure and development of biofilms. Using the channels modified by silane reagent, the culture time of biofilms (30 h) was reduced by 6 h compared to unmodified channels. With the addition of graphene sheets (10 mg/L) in Luria-Bertani (LB) medium, the graphene sheets possessed a minimum inhibition rate of 3.23% against recombinant E. coil. The biofilms cultivated by the LB medium with added graphene sheets were stably formed in 20 h; the formation time was 33.33% shorter than that by LB medium without graphene. The developed method provides an efficient and simple approach for rapid preparation of catalytic biofilms in microchannel reactors. View Full-Text
Keywords: biofilm; microreactor; surface modification; nanomaterials; recombinant Escherichia coil biofilm; microreactor; surface modification; nanomaterials; recombinant Escherichia coil
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhu, C.-T.; Mei, Y.-Y.; Zhu, L.-L.; Xu, Y.; Sheng, S.; Wang, J. Recombinant Escherichia coli BL21-pET28a-egfp Cultivated with Nanomaterials in a Modified Microchannel for Biofilm Formation. Int. J. Mol. Sci. 2018, 19, 2590.

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