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Article

Identification of an Essential Cytoplasmic Region of Interleukin-7 Receptor α Subunit in B-Cell Development

1
Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA
2
Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan
3
Toho University Graduate School of Medicine, Tokyo 143-8540, Japan
4
Department of Urology, Toho University Omori Medical Center, Tokyo 143-8541, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Current address: Department of Microbiology, Faculty of Medicine, Yamanashi University, Chuo 409-3898, Japan.
Int. J. Mol. Sci. 2018, 19(9), 2522; https://doi.org/10.3390/ijms19092522
Received: 29 June 2018 / Revised: 13 August 2018 / Accepted: 20 August 2018 / Published: 25 August 2018
Interleukin-7 (IL-7) is essential for lymphocyte development. To identify the functional subdomains in the cytoplasmic tail of the IL-7 receptor (IL-7R) α chain, here, we constructed a series of IL-7Rα deletion mutants. We found that IL-7Rα-deficient hematopoietic progenitor cells (HPCs) gave rise to B cells both in vitro and in vivo when a wild-type (WT) IL-7Rα chain was introduced; however, no B cells were observed under the same conditions from IL-7Rα-deficient HPCs with introduction of the exogenous IL-7Rα subunit, which lacked the amino acid region at positions 414–441 (d414–441 mutant). Signal transducer and activator of transcription 5 (STAT5) was phosphorylated in cells with the d414–441 mutant, similar to that in WT cells, in response to IL-7 stimulation. In contrast, more truncated STAT5 (tSTAT5) was generated in cells with the d414–441 mutant than in WT cells. Additionally, the introduction of exogenous tSTAT5 blocked B lymphopoiesis but not myeloid cell development from WT HPCs in vivo. These results suggested that amino acids 414–441 in the IL-7Rα chain formed a critical subdomain necessary for the supportive roles of IL-7 in B-cell development. View Full-Text
Keywords: interleukin-7; interleukin-7 receptor; B-cell development; signal transducer and activator of transcription 5 interleukin-7; interleukin-7 receptor; B-cell development; signal transducer and activator of transcription 5
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MDPI and ACS Style

Kasai, H.; Kuwabara, T.; Matsui, Y.; Nakajima, K.; Kondo, M. Identification of an Essential Cytoplasmic Region of Interleukin-7 Receptor α Subunit in B-Cell Development. Int. J. Mol. Sci. 2018, 19, 2522. https://doi.org/10.3390/ijms19092522

AMA Style

Kasai H, Kuwabara T, Matsui Y, Nakajima K, Kondo M. Identification of an Essential Cytoplasmic Region of Interleukin-7 Receptor α Subunit in B-Cell Development. International Journal of Molecular Sciences. 2018; 19(9):2522. https://doi.org/10.3390/ijms19092522

Chicago/Turabian Style

Kasai, Hirotake, Taku Kuwabara, Yukihide Matsui, Koichi Nakajima, and Motonari Kondo. 2018. "Identification of an Essential Cytoplasmic Region of Interleukin-7 Receptor α Subunit in B-Cell Development" International Journal of Molecular Sciences 19, no. 9: 2522. https://doi.org/10.3390/ijms19092522

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