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Int. J. Mol. Sci. 2018, 19(8), 2444; https://doi.org/10.3390/ijms19082444

Comparative Proteomic Analysis of Two Ralstonia solanacearum Isolates Differing in Aggressiveness

1
Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
2
College of Horticulture, South China Agricultural University, Guangzhou 510642, China
3
Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
4
Department of Plant Science, Weizmann Institute of Science, Rehovot 76100, Israel
These authors have contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 23 July 2018 / Revised: 14 August 2018 / Accepted: 17 August 2018 / Published: 18 August 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Ralstonia solanacearum is a soil-borne, plant xylem-infecting pathogen that causes the devastating bacterial wilt (BW) disease in a number of plant species. In the present study, two R. solanacearum strains with different degrees of aggressiveness―namely RsH (pathogenic to Hawaii 7996, a tomato cultivar resistant against most strains) and RsM (non-pathogenic to Hawaii 7996) were identified. Phylogenetic analysis revealed that both RsM and RsH belonged to phylotype I. To further elucidate the underlying mechanism of the different pathotypes between the two strains, we performed a comparative proteomics study on RsM and RsH in rich and minimal media to identify the change in the level of protein abundance. In total, 24 differential proteins were identified, with four clusters in terms of protein abundance. Further bioinformatics exploration allowed us to classify these proteins into five functional groups. Notably, the pathogenesis of RsM and RsH was particularly characterized by a pronounced difference in the abundance of virulence- and metabolism-related proteins, such as UDP-N-acetylglucosamine 2-epimerase (epsC) and isocitrate lyase (ICL), which were more abundant in the high pathogenicity strain RsH. Thus, we propose that the differences in pathogenicity between RsM and RsH can possibly be partially explained by differences in extracellular polysaccharide (EPS) and glyoxylate metabolism-related proteins. View Full-Text
Keywords: Ralstonia solanacearum; UDP-N-acetylglucosamine 2-epimerase (epsC); isocitrate lyase (ICL); pathogenesis; proteome Ralstonia solanacearum; UDP-N-acetylglucosamine 2-epimerase (epsC); isocitrate lyase (ICL); pathogenesis; proteome
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Wang, G.; Kong, J.; Cui, D.; Zhao, H.; Zhao, P.; Feng, S.; Zhao, Y.; Wang, W. Comparative Proteomic Analysis of Two Ralstonia solanacearum Isolates Differing in Aggressiveness. Int. J. Mol. Sci. 2018, 19, 2444.

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