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Open AccessArticle

Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation

1
Laboratory of Animal Reproduction, Department of Animal Science, Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan
2
NODAI Genome Research Center, Tokyo University of Agriculture, Setagaya, Tokyo 154-0017, Japan
3
Department of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Tochigi 329-0498, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(8), 2322; https://doi.org/10.3390/ijms19082322
Received: 16 July 2018 / Revised: 3 August 2018 / Accepted: 6 August 2018 / Published: 8 August 2018
(This article belongs to the Special Issue Reproductive Immunology: Cellular and Molecular Biology)
Background: Three-dimensional (3D) culture changes cell characteristics and function, suggesting that 3D culture provides a more physiologically relevant environment for cells compared with 2D culture. We investigated the differences in cell functions depending on the culture model in human trophoblast cells (Sw.71). Methods: Sw.71 cells were incubated in 2D monolayers or simple 3D spheroids. After incubation, cells were corrected to assess RNA-seq transcriptome or protein expression, and culture medium were corrected to detect cytokines. To clarify the role of actin cytoskeleton, spheroid Sw.71 cells were treated mycalolide B (inhibitor of actin polymerization) in a 3D culture. Results: RNA-seq transcriptome analysis, results revealed that 3D-cultured cells had a different transcriptional profile compared with 2D-cultured cells, especially regarding inflammation-related molecules. Although interleukin-6 (IL-6) mRNA level was higher in 3D-culured cells, its secretion levels were higher in 2D-cultured cells. In addition, the levels of mRNA and protein expression of regnase-1, regulatory RNase of inflammatory cytokine, significantly increased in 3D culture, suggesting post-translational modification of IL-6 mRNA via regnase-1. Treatment with mycalolide B reduced cell-to-cell contact to build 3D formation and increased expression of actin cytoskeleton, resulting in increased IL-6 secretin. Conclusion: Cell dimensionality plays an essential role in governing the spatiotemporal cellular outcomes, including inflammatory cytokine production and its negative regulation associated with regnase-1. View Full-Text
Keywords: inflammation; interleukin-6; regnase-1; trophoblast cells inflammation; interleukin-6; regnase-1; trophoblast cells
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MDPI and ACS Style

Seno, K.; Munakata, Y.; Sano, M.; Kawahara-Miki, R.; Takahashi, H.; Ohkuchi, A.; Iwata, H.; Kuwayama, T.; Shirasuna, K. Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation. Int. J. Mol. Sci. 2018, 19, 2322. https://doi.org/10.3390/ijms19082322

AMA Style

Seno K, Munakata Y, Sano M, Kawahara-Miki R, Takahashi H, Ohkuchi A, Iwata H, Kuwayama T, Shirasuna K. Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation. International Journal of Molecular Sciences. 2018; 19(8):2322. https://doi.org/10.3390/ijms19082322

Chicago/Turabian Style

Seno, Kotomi; Munakata, Yasuhisa; Sano, Michiya; Kawahara-Miki, Ryouka; Takahashi, Hironori; Ohkuchi, Akihide; Iwata, Hisataka; Kuwayama, Takehito; Shirasuna, Koumei. 2018. "Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation" Int. J. Mol. Sci. 19, no. 8: 2322. https://doi.org/10.3390/ijms19082322

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